Cellecta - Custom RNAi Services: shRNA Libraries, Functional Screening

Products and Services

PRODUCTS Pre-made shRNA Libraries

SERVICES shRNA Libraries Validated shRNA Libraries HT RNAi Screen Stable Cell Lines Pathway Analysis

Custom Stable Cell Lines

Using our lentiviral technology, we can generate several types of stable cell lines using GFP, Luciferase, or other markers, and optionally use tet-inducible expression. The service covers some "exotic" cell lines as well as the more common cell lines. Cells that are difficult to grow, such as primary cells, will require additional cost and time. We typically deliver uncloned populations of cells in 1.5-2 months at prices ranging from $10,000 to $20,000, and clonal cell lines in 3-4 months at prices ranging from $20,000 to $30,000. In comparison, other lentiviral stable cell line service providers charge up to $80,000 per cell line.

The benefits of using lentiviral technology over traditional methods for stable cell line generation are listed below.

Any cell type can be used, including cells that are difficult to transfect
An unlimited number of stable clones can be generated, so you have the freedom to choose the best producers or reporter cell lines by FACS
It is the only way to generate overproducer cell lines (for recombinant proteins) with multiple integrated expression constructs of transgene

Inducible Pluripotent Stem (iPS) cell lines NEW

Cellecta now offers a custom iPS cell line generation service to provide researchers with a novel alternative to embryonic stem cells. By introducing combinations of 4 transcriptional factors to the cells of your choice via lentiviral vectors, we create a clonal population of iPS cells. These cells function as closely as currently possible to ES cells.^1,2

Transcriptional factor-dependent Reporter cell lines

Used for your high-throughput functional RNAi and phage display screens, and HTS of chemical libraries. The main benefit of using lentiviral technology in generating reporter cell lines is that expression of the reporter gene indicates activation of a given transcriptional response element (TRE) by the cognate transcriptional factor in the natural chromosomal environment rather than in the episomal state in the nucleoplasm as is the case for conventional plasmid-based TR vectors. Tandem copies of integration can be avoided, thus allowing for faithful promoter regulation. To order, you choose the cell line, the transcriptional factor you wish to study, and the reporter gene.

Knockdown (shRNA) cell lines

Just choose the gene you want to suppress, your cell line, and a reporter gene, and we can produce a stable knockdown cell line. We provide the shRNA design, cloning into the lentiviral vector of your choice, and verification of knockdown.

Overexpression (cDNA) cell lines

We clone a gene of your choice (up to a 3kb ORF) into a lentiviral vector, and transduce the cell line of your choice to create a stable cell line which overexpresses the gene. The service includes sequence confirmation and Western blot analysis (or biological activity).

References

Yamanaka S, et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. doi:10.1016/j.cell.2007.11.019 | PMID 18035408
Thomson JA, Yu J, et al. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science DOI: 10.1126/science.1151526 | PMID 18033853

Current Events

View our company brochure for a comprehensive overview of Cellecta's RNAi Screening and related services.