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Human Genome-Wide shRNA Library

Cellecta offers a Pooled Lentiviral Human Genome-Wide shRNA library targeting nearly all protein-encoding genes. This shRNA library is the most current pooled lentiviral shRNA library available on the market. It covers all genes in the human genome, and each gene is targeted by 8 hairpins. We have incorporated clonal barcodes to enable you to track growth, differentiation, or migration of specific cells containing a specific shRNA throughout your experiment.

For Genome-Wide Coverage in Human Cells

  • 3 Modules, each covering approximately 6,500 genes
  • Each gene is targeted by 8 hairpins for a total of approximately 55,000 hairpins per module
  • Modules are made with non-overlapping barcodes so that they can be combined to form a complete genome-wide shRNA library
  • Targets all (19,276) protein-encoding genes

Intelligent Barcode Design, Quality Data

  • 22-nt barcodes facilitate Next-Gen Sequencing data analysis and identification of functional shRNAs
  • Compatible with Illumina HiSeq, GAIIx, and NextSeq NGS systems
  • Barcodes are identified and converted to lists of genes/shRNA with enumerated barcode data
  • Amplification of shRNA hairpins is not required

Library Modules for Intelligent Loss-of-Function Screening

  • hGW Module 1 targets signal transduction genes
    • Members of major signal transduction pathways
    • Top-ranking genes from the Cancer Genome Atlas
    • Approved drug targets
    • Many disease-associated genes
  • hGW Module 2 targets disease-associated genes and known drug targets
  • hGW Module 3 targets cell surface markers, extracellular matrix genes, and DNA binding genes

Available Formats

NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email orders@cellecta.com using your institutional email account, to ask for a quote with the discounted pricing.

Plasmid Library

Quantity provided in each library: 200 ug plasmid for individual modules, and 600 ug for 3 modules pooled

Catalog#NameCategoryPriceBuy
HGW-M1-P2hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (plasmid)$7,500.00
HGW-M2-P2hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (plasmid)$7,500.00
HGW-M3-P2hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (plasmid)$7,500.00
HGW-M123-P2hGW 55K Modules 1, 2, & 3 (Human Genome-wide shRNA Library, 3 Modules) (plasmid)$19,000.00
HGW-P2hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (plasmid)$9,000.00

Packaged Viral Library

Quantity provided in each library: 2 x 10^8 TU or 1 x 10^9 TU packaged

Catalog#NameCategoryPriceBuy
HGW-M1-V9hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)$18,000.00
HGW-M1-V8hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)$8,500.00
HGW-M2-V9hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)$18,000.00
HGW-M2-V8hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)$8,500.00
HGW-M3-V9hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (virus)$18,000.00
HGW-M3-V8hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (virus)$8,500.00
HGW-V9hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (virus)$35,000.00
HGW-V8hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (virus)$12,000.00

We can also make custom shRNA libraries according to your specifications. Please email sales@cellecta.com for details.

 

User Manual

  • RNAi Pooled Lentiviral shRNA Libraries (Online Manual or PDF)
  • NOTE: This User Manual replaces all previous shRNA library manuals.

Gene Lists

Vector Information

shRNA Library VectorNGS CassetteSequence (.txt | .gbk | .dna*)Map
pRSI16cb-U6-sh-13kCB18-HTS6cb-UbiC-TagRFP-2A-PuroHTS6cbFASTA | GenBank | SnapGeneMap

Product Certificates

Lot NumberCatalog NumberDescriptionQuantityProduct Certificate
18011146HGW-M2-V8hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)2 x 108 TUPDF
18010454HGW-V8hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (virus)2 x 108 TUPDF
17071103HGW-M1-V8hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)2 x 108 TUPDF
17030103HGW-M3-V8hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (virus)2 x 108 TUPDF
16102095HGW-P2hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (plasmid)600 µgPDF
15051321HGW-M1-V8hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)2 x 108 TUPDF
14110601HGW-M2-V8hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)2 x 108 TUPDF
14103001HGW-M2-V8hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)2 x 108 TUPDF
14101601HGW-M1-V8hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)2 x 108 TUPDF
14060501HGW-M2-V8hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)2 x 108 TUPDF
14021309HGW-V9hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (virus)1 x 109 TUPDF
13101104HGW-M123-P2hGW 55K Modules 1, 2, & 3 (Human Genome-wide shRNA Library, 3 Modules) (plasmid)200 µg eachPDF
13101103HGW-M123-P2hGW 55K Modules 1, 2, & 3 (Human Genome-wide shRNA Library, 3 Modules) (plasmid)200 µg eachPDF
13101103HGW-M2-P2hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (plasmid)200 µgPDF
13101102HGW-M3-V8hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (virus)2 x 108 TUPDF
13101009HGW-M3-V8hGW 55K Module 3 (Human Genome-wide shRNA Library, Module 3) (virus)2 x 108 TUPDF
13101008HGW-M2-V9hGW 55K Module 2 (Human Genome-wide shRNA Library, Module 2) (virus)1 x 109 TUPDF
13020404HGW-V9hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (virus)1 x 109 TUPDF
13020403HGW-M1-V8hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (virus)2 x 108 TUPDF
12121403HGW-P2hGW 165K Pool (Complete Human Genome-wide shRNA Library, Modules 1,2,3 Pooled) (plasmid)600 µgPDF
12121402HGW-M123-P2hGW 55K Modules 1, 2, & 3 (Human Genome-wide shRNA Library, 3 Modules) (plasmid)200 µg eachPDF
12121402HGW-M1-P2hGW 55K Module 1 (Human Genome-wide shRNA Library, Module 1) (plasmid)200 µgPDF

Safety Data Sheet (SDS)

* Download the SnapGene® Viewer to open .dna format files.

Next-Gen Sequencing and Analysis

Cellecta libraries are provided with a complete protocol and all sequencing information to enable researchers to perform high throughput genetics screens and analysis. However, we also do provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells for each time point or treatment condition (one sample), and we extract DNA, amplify, sequence, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies barcodes
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerate shRNA counts from raw sequencing data
  • Additional bioinformatics and pathway analysis available

Please see the Next-Gen Sequencing and Analysis web page for additional information and how to order.

Catalog#NameCategoryPriceBuy
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix$340.00
LNGS-102NGS Prep Kit for shRNA Libraries in pRSI16cb/17cb (HGW) (or pRSI16/17)$875.00
LNGS-102-SPSupplementary Primer Set for LNGS-102 (12 Additional Index Primers)$475.00
LFVC1LentiFuge™ Viral Concentration Reagent$325.00
LTDR1LentiTrans™ Transduction Reagent (Polybrene for Transduction)$175.00
LTSET-GLentiPrep™ Lentiviral Reagent Set with GFP Control for Packaging and Transduction$750.00
LTSET-RLentiPrep™ Lentiviral Reagent Set with RFP Control for Packaging and Transduction$750.00