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The CRISPR/Cas9 system can be used to knock out gene expression in vivo or in vitro by using a combination of an sgRNA (single guide RNA, or gRNA) along with a Cas9 nuclease. With clonal selection you can obtain permanent 100% knockout in your cells.
Achieve complete knockout of your gene with our reliable and precise CRISPR lentiviral constructs, available as plasmids or packaged, transduction-ready, lentiviral particles. Pick your gene of interest, choose your options, and we do the rest.
As an alternative, choose your gene of interest and cell background, and Cellecta will build a custom knockout, knock-in, or other engineered cell line for you.
Cellecta can provide either constitutive or tet-inducible sgRNA constructs targeting your gene of interest with a range of selection markers. Just let us know your target and we can design and clone sgRNA for CRISPR knockout (KO), CRISPRa, or CRISPRi. Vector options are available for both all-in-one constructs containing the Cas9 gene (single-vector) or sgRNA-only formats. Get the CRISPR constructs you want, simply and easily.
We can also make and provide you with a verified knockout or knock-in cell line. Engineered cell lines are validated by genomic sequence verification, and we offer a "Zero Footprint" procedure using transient Cas9 and sgRNA to make the knockout line.
Three tet-inducible sgRNA constructs targeting GFP were transduced in cells stably expressing both GFP and Cas9. After addition of doxycycline, over 80% of the GFP is knocked out.
The CRISPR/Cas9 system can be used for gene knockout (KO), knockdown, activation or to initiate knock-ins in vivo or in vitro by using a combination of an sgRNA (single-guide RNA) together with a Cas9 nuclease.
Developments of the CRISPR/Cas9 system have engineered the Cas9 protein to remove the nuclease and substitute other functions such as transcription repressor proteins (e.g., KRAB) or activator complexes (e.g., p53) to make a system that modulates gene activity.
Our custom sgRNA construction and CRISPR cell line services can be tailored to meet your experimental requirements. Just contact us and let us know what type of constructs or cells you need.
