Drug Target Discovery and Gene Function Analysis with RNAi Library Screening
Using pooled lentiviral-based shRNA libraries, Cellecta has developed an effective, scalable, high-throughput platform for broad range screening and identification of gene function. RNAi genetic screening makes use of discrete stable suppression of specific genes on a large-scale, allowing for loss-of-function screens in mammalian cell systems. RNAi genetic screens provide an unbiased approach to identify genes that act in specific cellular pathways.
RNAi Screening Services
We will perform a pooled lentiviral-based shRNA screening and deliver the end product whether it is a custom pooled shRNA library, treated cells, sub‑libraries, individual constructs, raw data, and/or analysis. Full-scale screening order typically involves the following steps:
- Custom design and construction of pooled shRNA library
- Functional testing of cells for specific phenotype or cellular response
- Library transduction and functional testing of cells
- Preparation and sequencing of samples from various assay points
- Data assembly and analysis
The turnaround time and pricing for screening varies, depending on the specifics (e.g., time points, growth rate of cells, size of library, etc.). However, a full HT RNAi screening typically takes 4-5 months and costs $70,000 to $90,000 for each cell line screened with a 27K shRNA library. If you are interested in screening, please contact us and we can discuss scenarios and provide details regarding various options.
Contact us at sales@cellecta.com for a custom quote. For answers to common questions about our pooled shRNA libraries, visit our Frequently Asked Questions (FAQs) page.
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