Custom Lentiviral Cloning

Cellecta provides complete lentiviral vector cloning services for construction of shRNA controls, reporters, and expression plasmids. As a result of our lentiviral experience, we can offer suggestions and recommendations to assist in designing the most effective constructs for a variety of applications.

Features of Custom Lentiviral Constructs

Select GFP, RFP, PuroR, BleoR, NeoR, or Hygro-HK markers
Incorporate UbiC, EF1, PGK, or CMV promoters
Choose constitutive shRNA promoters H1 or U6, or Tet-regulated H1
Constructs provided in arrayed or individual tube format
Obtain constructs as plasmid or packaged lentiviral particles

Custom shRNA Construction

For shRNA constructs, we only need the RefSeq sequence and we can design any number of shRNA constructs that target the transcript. Our proprietary shRNA design delivers approximately 55-70% functional shRNA (>70% knockdown) depending on the cell type.

Validated Knockdown Constructs

Custom shRNA constructs can also be functionally validated by qPCR in the cell line of your choice and under the conditions of your choice.

Order Number	Description	Amount
CVSHC-PS	Lentiviral shRNA Knockdown Construct	> 25 μg
CVPRC-PS	Lentiviral Expression Construct	> 25 μg
CVRPC-PS	Lentiviral Reporter Construct	> 25 μg
CVSHC-KO	shRNA Construct testing/Validation	1 x 10⁷ TU