Pooled Lentiviral shRNA Libraries for Identification of Drug Targets
Cellecta has the expertise and capability to generate high quality pooled libraries consisting of virtually any defined sequences up to 200 bases in length. This capability combined with our proprietary vectors and lentiviral expertise provides Cellecta with the ideal platform to create excellent quality, highly representative Pooled Lentiviral shRNA Libraries.
Pooled Lentiviral shRNA libraries of up to 55,000 discrete sequences can be provided in a variety of backbones with various selectable markers, and both constitutive and inducible promoters. Library construction (design, oligo synthesis, cloning, and quality control) takes approximately 3 months.
Features of shRNA Libraries
- Optimized shRNAs combining validated shRNA sequences and a predictive algorithm that produces more than 65% of shRNAs that generate greater than 70% knockdown
18-nt bar code compatible with the Illumina HT Sequencing platform to facilitate positive identification of shRNAs- HT sequencing QC that ensures presence and distribution of shRNAs for representation screening
- Lentiviral vectors for efficient delivery of high complexity, pooled shRNA libraries into a wide range of cell types
Typical library construction involves three services:
- oligo/library insert design,
- library insert synthesis, and
- library construction and sequencing for quality control
| Order # | Lentiviral shRNA Library | # of Target mRNAs |
|---|---|---|
| CPLVSHL-1K4 | 1.5K shRNA Library (min. order of 4) | 50 – 250 |
| CPLVSHL-6 | 6.5K shRNA Library | 100 – 1,000 |
| CPLVSHL-13 | 13K shRNA Library | 1,000 – 2,000 |
| CPLVSHL-27 | 27K shRNA Library | 2,500 – 5,000 |
| CPLVSHL-55 | 55K shRNA Library | 5,000 – 10,000 |
After construction, libraries can be provided directly as pooled DNA (>200 μg) or packaged and ready-for-use. Enough packaged library is provided for more than 20 screens at 200X representation and enough library DNA is provided to generate virus for over 100 screens.
Pre-Designed shRNA Libraries
Cellecta has defined libraries targeting specific populations of genes. These previously designed and optimized sets of shRNA are available as 27K Libraries of Pre‑Designed shRNAs. The pre-designed shRNA is simply synthesized and cloned into the lentiviral library vector of your choice. This option provides a proven library at a cost that is more economical than a full-scale custom service since there is no design step.
| Pre-Designed 27K shRNA Library |
|---|
| 4,000 Disease-Associated Genes |
| 5,000 Signaling Pathway Genes |
| 5,000 Cancer-Specific Genes |
| 5,000 Drug-Associated Genes |
Note: Mouse and Rat versions of each pre-designed library set are also available.
DECIPHER™ Pooled Lentiviral shRNA Libraries
As part of the open-source DECIPHER Project, Cellecta is making a series of pooled lentiviral shRNA libraries publicly available at no charge to academic researchers. The DECIPHER Project funded by NIH grants and in collaboration with the Roswell Park Cancer Institute aims to create a standardized platform to generate, analyze, and compare comprehensive shRNA knockdown screens from various studies. Plasmid versions of the DECIPHER libraries are available under an MTA that ensures data generated using these libraries is made publicly available. For more information on these libraries, visit the DECIPHER Project website at www.decipherproject.net.
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