Custom Knockdown, Reporter, or Expression Cell Lines
Lentiviral technology enables Cellecta to efficiently generate stable expression and reporter cell lines in virtually any type of cell, including cells that are difficult to grow and primary cells. An almost unlimited number of stable clones can be produced which facilitates selection of very high expressers and the most robust reporters.
Features of Lentiviral-Generated Cell Lines
- Cell lines can be made from nearly any cell type, including cells that are difficult to transfect
Expression or reporter construct is integrated in the natural chromosomal
environment rather than episomally in the nucleoplasm- It is the only way to generate overproducer cell lines (for recombinant proteins) with multiple integrated expression constructs of transgene
Custom Reporter Line
Transcriptional factor-dependent reporter cell lines can be ideal tools for high-throughput RNAi, peptide, and chemical library screens. Effective and specific stable transcriptional reporter cell lines can be generated with GFP, RFP, PuroR, BleoR, or other readout markers.
Custom Protein Expression Line
For expression constructs, an ORF of up to 3kb can be cloned into any of our cDNA expression vectors with the marker of your choice. After transduction and selection, we identify a stable cell line overexpressing the gene of interest. The service includes sequence confirmation and Western blot analysis or assay for biological activity.
Custom shRNA Knockdown Line
Cells expressing shRNA to knock out virtually any genes can be provided by simply choosing the target gene, cell line, markers, promoters (tet-regulatable or constitutive shRNA expression). We will create and validate a stable knockdown cell line. We design multiple shRNA per gene, generate individual cell lines, measure knockdown activity by qRT-PCR, and expand the most promising candidates. Optionally, we can provide a monoclonal isolate.
| Order Number | Description |
|---|---|
| CSCL-TR-P | Stable Transcriptional Reporter Cell Line Construction |
| CSCL-CD-P | Stable Recombinant Protein Expression Cell Line Construction |
| CSCL-SH-P | Stable shRNA Knockdown Cell Line Construction |
Quoted price will depend on the options chosen and complexity of the project. At least 3 x 106 cells will be delivered. For more detailed information, please contact us at info@cellecta.com.
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