Customized Functionally-Validated shRNA Libraries
Technology
Functional Data & Presentations
- HT RNAi screen data
- Presentation (PPT, 2.5MB)
- Molecular Medicine TRI Conference 2010 Poster (PDF, 1.4MB)
Related Products & Services
At the heart of Cellecta's technological repertoire is the pooled lentiviral shRNA library. Using our innovative method which allows us to quickly construct a library, we are capable of generating libraries of any size. Combined with our proprietary vector and shRNA designs, our functionally validated (FV) pooled shRNA libraries are not only cost-effective, but they are also the most powerful and robust HT RNAi screening tools available on the market.
A result of over 5 years of research, our "pre-designed" pools of shRNAs are ready-for-ligation to any of our vectors to produce customized shRNA libraries. The table below provides a summary of Cellecta's FV pre-designed pooled shRNA libraries.
| Pre-Designed shRNA Libraries (Human only; Mouse available May 2010) |
# of target mRNAs | shRNA complexity |
|---|---|---|
| Druggable Genome | 8,000 | 25,000 |
| Signaling Pathways | 5,000 | 27,000 |
| Cancer-Specific | 5,000 | 27,000 |
| Disease-Specific | 5,000 | 27,000 |
If you would like us to inform you by email when the mouse libraries become available, please register.
The advantages of using Cellecta's screening method over other methods include:
- Proprietary ultra-high throughput oligonucleotide synthesis (chip-based) for construction of genome-wide shRNA libraries.
- Second generation functionally validated shRNA libraries based on a proprietary algorithm, database of validated shRNA, and vector and shRNA design.
- Use of lentiviral vectors for efficient delivery of high complexity, pooled shRNA libraries into cells.
- Cellecta libraries are bar-coded and can be used with HT sequencing on the Illumina platform (Cellecta can provide this service) or with custom arrays (Agilent Technologies) to identify shRNA which modulate phenotypic responses.
In the pooled format strategy, a library encoding a pooled set of packaged viral shRNA constructs is introduced into a population of identical cells under conditions where each transduced cell will express only a single gene-specific siRNA. Cells exhibiting the desired phenotypic changes are isolated and the shRNA constructs, presumably inducing the phenotypes, are recovered by PCR and identified by sequence analysis or microarray hybridization using bar-coding strategy, or designing siRNAs complementary to existing commercial microarrays.
An example of a typical experiment and resulting functional data is available in our High Throughput RNAi Screen data section. View our Frequently Asked Questions (FAQs) page for detailed information on our pooled lentiviral shRNA libraries.
