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Home > Resources > Articles

Articles

Cellecta's First-Generation Pooled Lentiviral shRNA Libraries

• Tsujii H, Eguchi Y, Chenchik A, Mizutani T, Yamada K, Tsujimoto Y (2010). "Screening of Cell Death Genes With a Mammalian Genome-wide RNAi Library." J Biochem. Apr 26. [Epub ahead of print] PubMed PMID: 20421362.
• Ossovskaya, V. S., G. Dolganov, et al. (2009). "Loss of function genetic screens reveal MTGR1 as an intracellular repressor of beta1 integrin-dependent neurite outgrowth." J Neurosci Methods 177(2): 322-33.
• Gumireddy, K., A. Li, et al. (2009). "KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer." Nat Cell Biol 11(11): 1297-304.
• Chen, Y., R. Cairns, et al. (2009). "Oxygen consumption can regulate the growth of tumors, a new perspective on the warburg effect." PLoS One 4(9): e7033.
• Xu, M., M. Takanashi, et al. (2009). "USP15 plays an essential role for caspase-3 activation during Paclitaxel-induced apoptosis." Biochem Biophys Res Commun 388(2): 366-71.
• Yeung, M. L., L. Houzet, et al. (2009). "A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication." J Biol Chem 284(29): 19463-73.
• Huang, X., J. Y. Wang, et al. (2008). "Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion." BMC Syst Biol 2: 49.
• Hattori, H., X. Zhang, et al. (2007). "RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells." Blood 110(2): 640-50.
• Hwang, G. W., T. Hayashi, et al. (2007). "siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells." J Toxicol Sci 32(5): 581-3.

Use of Pooled shRNA Libraries

• Schlabach, M. R., J. Luo, et al. (2008). "Cancer proliferation gene discovery through functional genomics." Science 319(5863): 620-4.
• Silva, J. M., K. Marran, et al. (2008). "Profiling essential genes in human mammary cells by multiplex RNAi screening." Science 319(5863): 617-20.
• Ngo, V. N., R. E. Davis, et al. (2006). "A loss-of-function RNA interference screen for molecular targets in cancer." Nature 441(7089): 106-10.
• Guryanova, O. A., M. Makhanov, et al. (2006). "Optimization of a Genome-Wide Disordered Lentivector-Based Short Hairpin RNA Library." Mol Biol 40(3): 396-405.
• Devi, G. R. (2006). "siRNA-based approaches in cancer therapy." Cancer Gene Ther 13(9): 819-29.
• Berns, K., E. M. Hijmans, et al. (2004). "A large-scale RNAi screen in human cells identifies new components of the p53 pathway." Nature 428(6981): 431-7.
• Paddison, P. J., J. M. Silva, et al. (2004). "A resource for large-scale RNA-interference-based screens in mammals." Nature 428(6981): 427-31.

shRNA Library Reviews

• Kassner, P. D. (2008). "Discovery of novel targets with high throughput RNA interference screening." Comb Chem High Throughput Screen 11(3): 175-84.
• Martin, S. E. and N. J. Caplen (2007). "Applications of RNA interference in mammalian systems." Annu Rev Genomics Hum Genet 8: 81-108.
• Echeverri, C. J. and N. Perrimon (2006). "High-throughput RNAi screening in cultured cells: a user's guide." Nat Rev Genet 7(5): 373-84.
• Moffat, J. and D. M. Sabatini (2006). "Building mammalian signalling pathways with RNAi screens." Nat Rev Mol Cell Biol 7(3): 177-87.
• Voorhoeve, P. M. and R. Agami (2003). "Knockdown stands up." Trends Biotechnol 21(1): 2-4.

Viability Screens & Synthetic Lethality

• Reinhardt, H. C., H. Jiang, et al. (2009). "Exploiting synthetic lethal interactions for targeted cancer therapy." Cell Cycle 8(19): 3112-9.
• McLaughlin-Drubin, M. E. and K. Munger (2009). "Oncogenic activities of human papillomaviruses." Virus Res 143(2): 195-208.
• Luo, J., M. J. Emanuele, et al. (2009). "A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene." Cell 137(5): 835-48.
• Scholl, C., S. Frohling, et al. (2009). "Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells." Cell 137(5): 821-34.
• Jiang, H., H. C. Reinhardt, et al. (2009). "The combined status of ATM and p53 link tumor development with therapeutic response." Genes Dev 23(16): 1895-909.
• Boudreau, R. L., I. Martins, et al. (2009). "Artificial microRNAs as siRNA shuttles: improved safety as compared to shRNAs in vitro and in vivo." Mol Ther 17(1): 169-75.
• Luo, B., H. W. Cheung, et al. (2008). "Highly parallel identification of essential genes in cancer cells." Proc Natl Acad Sci U S A 105(51): 20380-5.
• Kassner, P. D. (2008). "Discovery of novel targets with high throughput RNA interference screening." Comb Chem High Throughput Screen 11(3): 175-84.
• Schwartz, G. F., K. S. Hughes, et al. (2008). "Proceedings of the international consensus conference on breast cancer risk, genetics, & risk management, April, 2007." Cancer 113(10): 2627-37.
• Elliott, D. D., S. I. Sherman, et al. (2008). "Growth factor receptors expression in anaplastic thyroid carcinoma: potential markers for therapeutic stratification." Hum Pathol 39(1): 15-20.
• Fakhoury, J., G. A. Nimmo, et al. (2007). "Harnessing telomerase in cancer therapeutics." Anticancer Agents Med Chem 7(4): 475-83.
• Martin, S. E. and N. J. Caplen (2007). "Applications of RNA interference in mammalian systems." Annu Rev Genomics Hum Genet 8: 81-108.
• Carpten, J. D., A. L. Faber, et al. (2007). "A transforming mutation in the pleckstrin homology domain of AKT1 in cancer." Nature 448(7152): 439-44.
• Echeverri, C. J. and N. Perrimon (2006). "High-throughput RNAi screening in cultured cells: a user's guide." Nat Rev Genet 7(5): 373-84.
• Moffat, J. and D. M. Sabatini (2006). "Building mammalian signalling pathways with RNAi screens." Nat Rev Mol Cell Biol 7(3): 177-87.
• Moffat, J., D. A. Grueneberg, et al. (2006). "A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen." Cell 124(6): 1283-98.
• Brummelkamp, T. R., A. W. Fabius, et al. (2006). "An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors." Nat Chem Biol 2(4): 202-6.
• Shabalina, S. A., A. N. Spiridonov, et al. (2006). "Computational models with thermodynamic and composition features improve siRNA design." BMC Bioinformatics 7: 65.
• Taxman, D. J., L. R. Livingstone, et al. (2006). "Criteria for effective design, construction, and gene knockdown by shRNA vectors." BMC Biotechnol 6: 7.
• Schilling, S. H., M. B. Datto, et al. (2006). "A phosphatase controls the fate of receptor-regulated Smads." Cell 125(5): 838-40.
• Massague, J. and R. R. Gomis (2006). "The logic of TGFbeta signaling." FEBS Lett 580(12): 2811-20.
• An, D. S., F. X. Qin, et al. (2006). "Optimization and functional effects of stable short hairpin RNA expression in primary human lymphocytes via lentiviral vectors." Mol Ther 14(4): 494-504.
• Birmingham, A., E. M. Anderson, et al. (2006). "3' UTR seed matches, but not overall identity, are associated with RNAi off-targets." Nat Methods 3(3): 199-204.
• Cullen, B. R. (2006). "Induction of stable RNA interference in mammalian cells." Gene Ther 13(6): 503-8.
• Olson, A., N. Sheth, et al. (2006). "RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs." Nucleic Acids Res 34(Database issue): D153-7.
• Farmer, H., N. McCabe, et al. (2005). "Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy." Nature 434(7035): 917-21.
• MacKeigan, J. P., L. O. Murphy, et al. (2005). "Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance." Nat Cell Biol 7(6): 591-600.
• Silva, J. M., M. Z. Li, et al. (2005). "Second-generation shRNA libraries covering the mouse and human genomes." Nat Genet 37(11): 1281-8.
• Huesken, D., J. Lange, et al. (2005). "Design of a genome-wide siRNA library using an artificial neural network." Nat Biotechnol 23(8): 995-1001.
• Boese, Q., D. Leake, et al. (2005). "Mechanistic insights aid computational short interfering RNA design." Methods Enzymol 392: 73-96.
• Jagla, B., N. Aulner, et al. (2005). "Sequence characteristics of functional siRNAs." RNA 11(6): 864-72.
• Wu, G., M. Xing, et al. (2005). "Somatic mutation and gain of copy number of PIK3CA in human breast cancer." Breast Cancer Res 7(5): R609-16.
• Kaelin, W. G., Jr. (2005). "The concept of synthetic lethality in the context of anticancer therapy." Nat Rev Cancer 5(9): 689-98.
• Sachse, C., E. Krausz, et al. (2005). "High-throughput RNA interference strategies for target discovery and validation by using synthetic short interfering RNAs: functional genomics investigations of biological pathways." Methods Enzymol 392: 242-77.
• Silva, J., K. Chang, et al. (2004). "RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age." Oncogene 23(51): 8401-9.
• Zheng, L., J. Liu, et al. (2004). "An approach to genomewide screens of expressed small interfering RNAs in mammalian cells." Proc Natl Acad Sci U S A 101(1): 135-40.
• Mangeot, P. E., F. L. Cosset, et al. (2004). "A universal transgene silencing method based on RNA interference." Nucleic Acids Res 32(12): e102.
• Du, Q., H. Thonberg, et al. (2004). "Validating siRNA using a reporter made from synthetic DNA oligonucleotides." Biochem Biophys Res Commun 325(1): 243-9.
• Willingham, A. T., Q. L. Deveraux, et al. (2004). "RNAi and HTS: exploring cancer by systematic loss-of-function." Oncogene 23(51): 8392-400.
• Downward, J. (2004). "Use of RNA interference libraries to investigate oncogenic signalling in mammalian cells." Oncogene 23(51): 8376-83.
• Yuan, B., R. Latek, et al. (2004). "siRNA Selection Server: an automated siRNA oligonucleotide prediction server." Nucleic Acids Res 32(Web Server issue): W130-4.
• Zhao, J. J., O. V. Gjoerup, et al. (2003). "Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase." Cancer Cell 3(5): 483-95.
• Martin, A. C. and D. G. Drubin (2003). "Impact of genome-wide functional analyses on cell biology research." Curr Opin Cell Biol 15(1): 6-13.
• Voorhoeve, P. M. and R. Agami (2003). "Knockdown stands up." Trends Biotechnol 21(1): 2-4.
• Aza-Blanc, P., C. L. Cooper, et al. (2003). "Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening." Mol Cell 12(3): 627-37.
• Brummelkamp, T. R., S. M. Nijman, et al. (2003). "Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-kappaB." Nature 424(6950): 797-801.
• Kumar, R., D. S. Conklin, et al. (2003). "High-throughput selection of effective RNAi probes for gene silencing." Genome Res 13(10): 2333-40.
• Hahn, W. C., S. K. Dessain, et al. (2002). "Enumeration of the simian virus 40 early region elements necessary for human cell transformation." Mol Cell Biol 22(7): 2111-23.
• Tuschl, T. (2002). "Expanding small RNA interference." Nat Biotechnol 20(5): 446-8.
• Hannon, G. J. (2002). "RNA interference." Nature 418(6894): 244-51.
• Vogel, C. L., M. A. Cobleigh, et al. (2002). "Efficacy and safety of trastuzumab as a single agent in first-line treatment of HER2-overexpressing metastatic breast cancer." J Clin Oncol 20(3): 719-26.
• Naidu, R., N. A. Wahab, et al. (2002). "Expression and amplification of cyclin D1 in primary breast carcinomas: relationship with histopathological types and clinico-pathological parameters." Oncol Rep 9(2): 409-16.
• Elenbaas, B., L. Spirio, et al. (2001). "Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells." Genes Dev 15(1): 50-65.
• Ross, J. S. and J. A. Fletcher (1998). "The HER-2/neu oncogene in breast cancer: prognostic factor, predictive factor, and target for therapy." Stem Cells 16(6): 413-28.
• Steck, P. A., M. A. Pershouse, et al. (1997). "Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers." Nat Genet 15(4): 356-62.
• Seshadri, R., C. S. Lee, et al. (1996). "Cyclin DI amplification is not associated with reduced overall survival in primary breast cancer but may predict early relapse in patients with features of good prognosis." Clin Cancer Res 2(7): 1177-84.
• McCann, A. H., A. Kirley, et al. (1995). "Amplification of the MDM2 gene in human breast cancer and its association with MDM2 and p53 protein status." Br J Cancer 71(5): 981-5.
• Marchetti, A., F. Buttitta, et al. (1995). "mdm2 gene alterations and mdm2 protein expression in breast carcinomas." J Pathol 175(1): 31-8.
• Champeme, M. H., I. Bieche, et al. (1994). "Int-2/FGF3 amplification is a better independent predictor of relapse than c-myc and c-erbB-2/neu amplifications in primary human breast cancer." Mod Pathol 7(9): 900-5.
• Borg, A., B. Baldetorp, et al. (1992). "c-myc amplification is an independent prognostic factor in postmenopausal breast cancer." Int J Cancer 51(5): 687-91.