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HT RNAi Genetic Screens

Cellecta offers a comprehensive high throughput functional screening service using focused, genome-wide, or custom non-validated or functionally-validated pooled lentiviral shRNA libraries. Below are the options to choose from. We can perform any or all of the experimental steps for you.

Option 1. Customer performs the screen.

  • Cellecta...
  • Provides technical support to help design screening protocol with pooled shRNA library
  • Provides a clonal reporter cell line (if necessary), which can be purchased from Cellecta, compatible with the RNAi screen with pooled shRNA library
  • Performs data analysis: customer sends send us infected/treated cell samples, and we purify RNA/DNA, amplify shRNA inserts, perform HT sequencing and statistical analysis, and then send the proprietary data back to customer. (Cellecta does not own any rights to data—we perform services under a confidentiality agreement.)

Option 2. Cellecta performs the screen.

  • Cellecta...
  • Provides a custom reporter cell line (if necessary)
  • Transduces cells with the pooled shRNA library
  • Performs functional testing of cells with a specific phenotype or cellular response
  • Purifies RNA/DNA from control and selected samples
  • Amplifies shRNA inserts and performs hybridization and/or HT sequencing
  • Performs statistical analysis of the digital RNA screening data set

For Option 1, the typical time to completion is 2 months. Prices start at $1,500 - $2,500 per HT sequencing sample, and a minimum of 3 samples is required. The development of a clonal reporter cell line requires approximately 2-4 months and is priced between $30,000 and 50,000 depending on several factors, such as the vector, selection marker, and whether it is a clonal or non-clonal cell line.

Option 2, the complete functional screening service, may take up to 6 months and is priced at $50,000 and up depending on the complexity of the RNAi screen. The price will decrease as our level of involvement decreases.

We also offer additional, downstream services:

  • Pathway Analysis: We can overlap your data set with the pathways affected by each of the identified shRNA clones
  • Digital Gene Expression Profiling Service, from the purified RNA samples
  • Confirmation of RNAi screening studies, by performing a second round of RNAi screening with a sub-library generated from the results of the first screen
  • Generation of individual shRNA constructs, for validation studies

Feel free to contact us at orders@cellecta.com for a custom quote. We are now accepting collaborations to further your research by applying for joint NIH grants. For more information, please email us at collaborate@cellecta.com.

View our Frequently Asked Questions (FAQs) page for detailed information on our pooled lentiviral shRNA libraries.