Cellecta - HT RNAi Genetic Screens

HT RNAi Genetic Screens

Cellecta offers a comprehensive high throughput RNAi genetic screening service using our pre-designed validated or custom non-validated pooled lentiviral bar-coded shRNA libraries. We will perform as much of the screen as you require and deliver the end product whether it be a custom pooled shRNA library, treated cells, sub-libraries, individual constructs, raw data, and/or full bioinformatic and pathway analysis. Below are the most popular service options. We can perform any or all of the experimental steps for you. For an overview of the services we provide, visit the HT RNAi screen technology page. View the TGF-β and Prostate Cancer Viability screen experimental data pages for examples of HT RNAi screens.

Option A. Client performs the screen.
Cellecta...

Consults with Client, under Confidentiality Agreement, to determine the best plan of action.
Designs and constructs one or more Custom Pooled Lentiviral shRNA Libraries targeting client's list(s) of genes or a pre-defined list of genes from our Pre-Designed shRNA Libraries. (2-3 months)
Provides assistance in designing the optimal HT RNAi screening protocol (if necessary).
(Optional) Provides a clonal reporter cell line appropriate for Client's RNAi screen. (3-4 months, done in parallel with shRNA library construction)
(Optional) Performs data analysis. Client sends infected/treated cell samples, and we purify RNA/DNA, amplify shRNA-specific bar-codes, perform HT sequencing and statistical analysis, and send the proprietary data back to Client*. (1-2 months)

Option B. Cellecta performs the screen.
Cellecta...

Consults with Client, under Confidentiality Agreement, to determine the best plan of action.
Designs and constructs one or more Custom Pooled Lentiviral shRNA Libraries targeting client's list(s) of genes or a pre-defined list of genes from our Pre-Designed shRNA Libraries. (2-3 months)
(Optional) Develops a clonal reporter cell line appropriate for Client's RNAi screen. (3-4 months, done in parallel with shRNA library construction)
Transduces Client's target cell line with pooled shRNA library(ies) and performs functional testing of cells with a specific phenotype or cellular response. (1-2 months)
Performs data analysis. We purify RNA/DNA from infected/treated cell samples, amplify shRNA-specific bar-codes, perform HT sequencing and statistical analysis, and send the proprietary data back to Client*. (1-2 months)

* Cellecta does not own any rights to data—all data and IP are assigned to Client.

For Option A, the time to completion can vary from 2 to 4 months (steps 1-4). An additional 1 to 2 months is required for subsequent data analysis (step 5). The development of a clonal reporter cell line requires approximately 3-4 months and can be generated in parallel with pooled shRNA library construction. Complete sample preparation, HT sequencing, and statistical analysis of data can be performed, and with a minimum of 7 samples is required (two sets of triplicates and a control recommended).

Option B, the complete HT RNAi screening service, may take from 4 to 8 months (depending on options) and is priced depending on the complexity of the RNAi screen. The per-cell-line price will decrease as our level of involvement decreases and the requested number of screens increases.

We also offer additional, downstream services:

Pathway Analysis, by overlapping your data set with the pathways affected by each of the identified shRNA clones
Digital Gene Expression Profiling Service, starting from the purified RNA samples
Confirmation of RNAi screening studies, by performing a second round of RNAi screening with a sub-library generated from the results of the first screen
Generation of individual shRNA constructs, for validation studies

Contact us at sales@cellecta.com for a custom quote. For answers to common questions about our pooled shRNA libraries, visit our Frequently Asked Questions (FAQs) page. For relevant references and literature, visit the Articles page under Resources.