Custom Genome-Wide and Focused shRNA Libraries
Technology
Functional Data & Presentations
- HT RNAi screen data
- Ariadne/Cellecta flyer (PDF, 0.4MB)
- Presentation (PPT, 2.5MB)
- Molecular Medicine TRI Conference 2010 Poster (PDF, 1.4MB)
Related Products & Services
At the heart of Cellecta's technological repertoire is the pooled lentiviral shRNA library. Using our innovative method which allows us to quickly construct a library, we are capable of generating libraries of any size on a custom basis. In 1-2 months, depending on the complexity of the library you desire, we provide you with the pooled, lentiviral packaged shRNA library, the list of shRNA sequences, and as much technical support you need to successfully plan and execute a functional screen. For ordering information, go to our Custom shRNA Library Order page.
The advantages of using Cellecta's screening method over other methods include:
- Low-cost technology for construction of focused or genome-wide pooled shRNA lentiviral libraries.
- Second generation functionally validated shRNA libraries based on proprietary ultra-high throughput validation technology.
- Cellecta's highly optimized shRNA structure increases target knockdown efficiency and reduces percentage of false negative hits.
- HT sequencing based on bar-coded, pooled lentiviral shRNA libraries improves positive identification of shRNAs which modulate phenotypic responses.
- Cellecta libraries can also be designed for compatibility with custom arrays (Agilent Technologies).
- Tet-regulated shRNA expression enhances efficiency of shRNA enrichment.
- Use of Lentiviral vectors for efficient delivery of high complexity, pooled shRNA libraries into cells.
In the pooled format strategy, a library encoding a pooled set of packaged viral shRNA constructs is introduced into a population of identical cells under conditions where each transduced cell will express only a single gene-specific siRNA. Cells exhibiting the desired phenotypic changes are isolated and the shRNA constructs, presumably inducing the phenotypes, are recovered by PCR and identified by sequence analysis or microarray hybridization using bar-coding strategy, or designing siRNAs complementary to existing commercial microarrays.
Fig. 1. Construction, screening, and data analysis of Cellecta's shRNA libraries.
An example of a typical experiment and resulting functional data is available in our High Throughput RNAi Screen data section. View our Frequently Asked Questions (FAQs) page for detailed information on our pooled lentiviral shRNA libraries.
