Transcriptional Reporter Cell Lines
Using lentiviral technology, we can generate stable transcriptional reporter cell lines using GFP, RFP, Puro, Bleo, or other readout markers. The service covers some "exotic" cell lines as well as the more common cell lines. Cells that are difficult to grow, such as primary cells, will require additional cost and time. We typically deliver uncloned populations of cells in 1.5-2 months and clonal cell lines in 3-4 months.
The benefits of using lentiviral technology over traditional methods for stable cell line generation are listed below.
- Expression of the reporter gene indicates transcriptional factor activation in the natural chromosomal environment rather than episomally in the nucleoplasm as is the case for plasmid-based cell lines
- Almost any cell type can be used, including cells that are difficult to transfect
- An unlimited number of stable clones can be generated, so you have the freedom to choose the best reporter cell lines by FACS
Transcriptional factor-dependent reporter cell lines can be ideal tools for high-throughput RNAi and peptide library screens, and HTS of chemical libraries. The main benefit of using lentiviral technology in generating reporter cell lines is that expression of the reporter gene indicates activation of a given transcriptional response element (TRE) by the cognate transcriptional factor in the natural chromosomal environment rather than in the episomal state in the nucleoplasm as is the case for conventional plasmid-based TR vectors. Tandem copies of integration can be avoided, thus allowing for faithful promoter regulation. To order, you choose the cell line, the transcriptional factor you wish to study, and the promoter/reporter gene combination.
Fig. 1. Reporter vector design and reporter activation data for various cellular responses in several cell lines.
To obtain a quote for a custom reporter cell line, contact us by email at sales@cellecta.com or by phone, toll-free at 877-938-3910, to let us know your requirements. Please refer to the list below for the choices available. You can discuss by phone or in person the details of the project, and we will prepare for you a customized reporter cell line agreement.
- Transcription factor to monitor
- Cloning vector from Cellecta, or your own
- Single or dual reporters: choose from GFP, RFP, PuroR, BleoR, or other
- Reporter promoter: CMV, EF1, PGK, UbiC or other
Quoted price will depend on the options chosen and complexity of the project. At least 3 x 10^6 cells will be delivered. For more detailed information, please contact us at info@cellecta.com
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