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Transcriptional Reporter Cell Lines

Using our lentiviral technology, we can generate transcriptional reporter cell lines using GFP, RFP, Puro, Bleo, or other readout markers. The service covers some "exotic" cell lines as well as the more common cell lines. Cells that are difficult to grow, such as primary cells, will require additional cost and time. We typically deliver uncloned populations of cells in 1.5-2 months at prices ranging from $15,000 to $25,000, and clonal cell lines in 3-4 months at prices ranging from $25,000 to $35,000. In comparison, some other lentiviral stable cell line service providers charge up to $80,000 per cell line.

The benefits of using lentiviral technology over traditional methods for stable cell line generation are listed below.

  • Any cell type can be used, including cells that are difficult to transfect
  • An unlimited number of stable clones can be generated, so you have the freedom to choose the best reporter cell lines by FACS

Transcriptional factor-dependent reporter cell lines can be used for your high-throughput functional RNAi and peptide library screens, and HTS of chemical libraries. The main benefit of using lentiviral technology in generating reporter cell lines is that expression of the reporter gene indicates activation of a given transcriptional response element (TRE) by the cognate transcriptional factor in the natural chromosomal environment rather than in the episomal state in the nucleoplasm as is the case for conventional plasmid-based TR vectors. Tandem copies of integration can be avoided, thus allowing for faithful promoter regulation. To order, you choose the cell line, the transcriptional factor you wish to study, and the reporter gene.

Reporter Cell Line activation data

Reporter vector design and reporter activation data for various cellular responses in several cell lines.