HT RNAi Genetic Screen Technology
The basis of Cellecta's HT RNAi genetic screening technique is the stable suppression of specific genes on a large-scale, allowing for loss-of-function screens in mammalian cell systems. High-throughput genetic screens (HTGS) can be utilized to investigate any aspect of biology that can be recapitulated in a cell culture model. As opposed to expressing and assaying the functional effects of an individual siRNA molecule, development of complex shRNA libraries allows for simultaneous screening of thousands of different shRNA molecules on a target population. In general, forward genetic screens represent an unbiased approach to identify genes that act in specific cellular pathways. The target is genetically inhibited, which is analogous to the way in which a drug blocks its target.
HT RNAi genetic screens have been proven to be an extremely potent and versatile tool to explore the molecular basis of cancer development and progression, and to discover genes essential for viability in cancer cells that can be used as targets for anticancer drug development.
Fig. 1. Flowchart of Cellecta's HT RNAi Screen services, including construction of pooled bar-coded shRNA libraries, functional screen and phenotypic selection, and bioinformatic data and pathway analysis.
For detailed information about our functional screening service, please see the High-Throughput RNAi Functional Screening section under Services.
View our Frequently Asked Questions (FAQs) page for answers to common questions about our pooled lentiviral shRNA libraries. For relevant references and literature, visit the Articles page under Resources.
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