LOCUS Exported 11553 bp ds-DNA circular SYN 17-MAR-2016 DEFINITION . ACCESSION . VERSION . KEYWORDS Cellecta-pRSGCUP-U6-sg-HTS6C-UbiC-Cas9-2A-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11553) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 17, 2016 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11553 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 6..232 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" /note="color: #ffffff; direction: RIGHT" LTR 233..413 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 460..585 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1078..1311 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" promoter 1819..2059 /note="U6 promoter" /note="RNA polymerase III promoter for human U6 snRNA" /note="color: #ffffff; direction: RIGHT" misc_feature 2065..2094 /note="sgRNA cloning site" /note="color: #ffff00" misc_feature 2122..2237 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /note="color: #ffe4c4" promoter 2303..2702 /note="UbiC promoter" /note="human ubiquitin C promoter" /note="color: #ffffff; direction: RIGHT" CDS 2721..2741 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2745..2765 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2769..2789 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2796..6896 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Cas9" /note="generates RNA-guided double strand breaks in DNA" /note="color: #993366" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6897..6944 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /note="nucleoplasmin NLS" /note="color: #993366" /translation="KRPAATKKAGQAKKKK" CDS 6960..7013 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 7010" /translation="EGRGSLLTCGDVEENPGP" CDS 7020..7619 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 7632..8220 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(8103..8114) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 8102" /translation="IEGR" LTR 8294..8527 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 8599..8720 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin 8760..8895 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" primer_bind complement(8928..8944) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: LEFT" protein_bind 8952..8968 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" promoter complement(8976..9006) /note="lac promoter" /note="promoter for the E. coli lac operon" /note="This reverse directional feature has 3 segments: 1: 8976 .. 8982 / #ffffff / -10 2: 8983 .. 9000 / #ffffff 3: 9001 .. 9006 / #ffffff / -35" protein_bind 9021..9042 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." /note="color: #31849b" rep_origin complement(9330..9918) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(10089..10949) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 10089 .. 10880 / #ccffcc 2: 10881 .. 10949 / #ccffcc / signal sequence Cleavage site after base 10880" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10950..11054) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" primer_bind 11528..11544 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga 1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta 1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt 2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg 2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg 2281 aattcatcac cggttcttcg aaggcctccg cgccgggttt tggcgcctcc cgcgggcgcc 2341 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct 2401 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag 2461 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt 2521 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct 2581 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt 2641 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg 2701 gtctagccgc caccatggcc ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa 2761 aggtagatcc aaaaaagaag agaaaggtag gtggagacaa gaagtacagc atcggcctgg 2821 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca 2881 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag 2941 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa 3001 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga 3061 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg 3121 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg 3181 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg 3241 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga 3301 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc 3361 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg 3421 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc 3481 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc 3541 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca 3601 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt 3661 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca 3721 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc 3781 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga 3841 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg 3901 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc 3961 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca 4021 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt 4081 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct 4141 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg 4201 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga 4261 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca 4321 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg 4381 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg 4441 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca 4501 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct 4561 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg 4621 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag 4681 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc 4741 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca 4801 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca 4861 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga 4921 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca 4981 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag 5041 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca 5101 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag 5161 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga 5221 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca 5281 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg 5341 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg 5401 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca 5461 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg 5521 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc 5581 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga 5641 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt 5701 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga 5761 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt 5821 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg 5881 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga 5941 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg 6001 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc 6061 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta 6121 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga 6181 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg 6241 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg 6301 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag 6361 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc 6421 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct 6481 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg 6541 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca 6601 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag 6661 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca 6721 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca 6781 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc 6841 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa 6901 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggcggcc gcaggatccg 6961 agggcagagg aagccttcta acatgcggtg acgtggagga gaatcccggc ccttccggga 7021 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 7081 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 7141 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 7201 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 7261 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 7321 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 7381 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 7441 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 7501 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 7561 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 7621 tctaggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 7681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 7741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 7801 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 7861 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 7921 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7981 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 8041 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 8101 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 8161 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc tctttgggcc gcctccccgc 8221 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 8281 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct 8341 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 8401 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 8461 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 8521 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 8581 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 8641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 8701 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 8761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 8821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 8881 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt 8941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 9721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 9781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 9841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 9901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 9961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 10741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 10801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 10861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 10921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 10981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 11101 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 11161 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 11221 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 11281 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 11341 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc 11401 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 11461 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 11521 cgacgttgta aaacgacggc cagtgccaag ctg //