LOCUS       pRSI9-U6   7500 bp    DNA   circular            29-OCT-2004
SOURCE      
  ORGANISM  
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|pRSI9-U6|
COMMENT     VNTAUTHORNAME|Cellecta|
COMMENT     VNTOAUTHORNAME|Tel: (650) 938-3910|
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FEATURES             Location/Qualifiers
     promoter        7..233
                     /vntifkey="30"
                     /label=RSV\promoter
     misc_feature    4546..4677
                     /vntifkey="21"
                     /label=SV40\EEL\poly(A)s
     misc_feature    2194..2311
                     /vntifkey="21"
                     /label=cPPT
     misc_feature    4707..4853
                     /vntifkey="21"
                     /label=SV40\Ori
     CDS             complement(6036..6896)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    5277..5277
                     /vntifkey="21"
                     /label=ORI
     misc_feature    569..922
                     /vntifkey="21"
                     /label=Gag
     LTR             233..330
                     /vntifkey="19"
                     /label=5'R
     LTR             331..413
                     /vntifkey="19"
                     /label=5'U5
     misc_feature    1072..1370
                     /vntifkey="21"
                     /label=RRE
     misc_feature    1371..1787
                     /vntifkey="21"
                     /label=ENV
     promoter        1803..2064
                     /vntifkey="30"
                     /label=U6\promoter
     promoter        2360..2758
                     /vntifkey="29"
                     /label=Ubi\Promoter
     misc_feature    3489..3542
                     /vntifkey="21"
                     /label=T2A
     misc_feature    3549..4145
                     /vntifkey="21"
                     /label=puroR
     LTR             4176..4489
                     /vntifkey="19"
                     /label=3dLTR
     misc_feature    2772..3482
                     /vntifkey="21"
                     /label=TagRFP
     misc_feature    2099..2106
                     /vntifkey="21"
                     /label=IND
     primer          2130..2151
                     /vntifkey="27"
                     /label=Gex2M
     primer          1917..1939
                     /vntifkey="27"
                     /label=Fwd-U6-2
     primer          1863..1888
                     /vntifkey="27"
                     /label=Fwd-U6-1
     primer          complement(2091..2116)
                     /vntifkey="27"
                     /label=GexSeqN
     primer          complement(2107..2132)
                     /vntifkey="27"
                     /label=GexSeqM
     primer          2156..2179
                     /vntifkey="27"
                     /label=Rev-CPPT-5
BASE COUNT     1964 a      1846 c      1985 g      1705 t 
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga 
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta 
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcgtgcgtt 
     2101 tggggtttcg gactgtagaa ctctgaacct ctcggtggtc gccgtatcat tagaattctc 
     2161 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg 
     2221 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 
     2281 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 
     2341 actttggccg ccgttcgaag gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc 
     2401 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc 
     2461 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat 
     2521 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca 
     2581 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg 
     2641 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg 
     2701 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtc 
     2761 tagacgccac catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc 
     2821 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca 
     2881 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct 
     2941 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca 
     3001 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag 
     3061 tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg 
     3121 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga 
     3181 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg 
     3241 gcctggaagg cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca 
     3301 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct 
     3361 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc 
     3421 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta 
     3481 atggatccga gggcagagga agccttctaa catgcggtga cgtggaggag aatcccggcc 
     3541 cttccgggat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 
     3601 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 
     3661 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 
     3721 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 
     3781 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 
     3841 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 
     3901 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 
     3961 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 
     4021 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 
     4081 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 
     4141 gtgcctgaat ctaggtcgac attctacttg gtacctttaa gaccaatgac ttacaaggca 
     4201 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     4261 caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     4321 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     4381 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     4441 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     4501 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     4561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     4621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     4681 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     4741 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     4801 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacc 
     4861 aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     4921 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     4981 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     5041 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     5101 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     5161 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     5221 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     5281 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     5341 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5401 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     5461 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     5521 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     5581 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     5641 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     5701 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     5761 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     5821 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     5881 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     5941 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     6001 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     6061 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     6121 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     6181 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     6241 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     6301 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     6361 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     6421 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     6481 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     6541 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     6601 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     6661 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     6721 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     6781 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     6841 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     6901 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     6961 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     7021 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
     7081 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
     7141 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
     7201 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 
     7261 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 
     7321 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 
     7381 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 
     7441 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg 
//
