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Combining Next-Generation Sequencing (NGS) Barcoding Technologies and CRISPR sgRNA Libraries with Targeted Gene Expression Profiling for Single-Cell Genetic Analysis

Pooled lentiviral libraries of CRISPR sgRNA to mediate genome-wide gene knockout have become an invaluable tool for uncovering the functional genetic drivers required for a biological response. Another type of pooled lentiviral library designed with unique DNA sequence tags has been used to label large populations of cells with unique cell-specific barcodes which allows monitoring changes in sub-populations of cells with distinct phenotypes over time. Further, these barcode lentiviral libraries can be designed so that the unique barcode sequence is detectable in next-generation sequencing (NGS) RNA expression profiling assays (e.g., RNA-seq). As a result, investigators can analyze and link distinct molecular changes to sub-populations of cells with distinct gene expression profiles and phenotypes, such as drug resistance. The additional step of incorporating these cell barcodes together with genetic effectors, such as CRISPR sgRNA libraries, make it possible to tease out gene expression changes that result from specific genetic disruptions, and link these to the development of specific phenotypes. Cellecta will discuss work we are doing to develop this integrated platform that combines CRISPR sgRNA screens with single-cell genetic analysis.

Join us for our Tech Talk at the 2018 ASCB/EMBO Annual Meeting

Where: Theater 1, Learning Center, San Diego Convention Center

When: Monday, December 10, 2018 — 9:30 to 10:30 am

9:20 am   Registration and Refreshments

9:30 – 9:50 am  Introduction – Cellecta Technologies: Your Bridge to Discovery – Paul Diehl, Ph.D., COO, Cellecta

9:50 – 10:20 am  NGS Barcoding Technologies and Genome-Wide Targeted Expression Profiling for Single-Cell Genetic Analysis – Alex Chenchik, Ph.D., President & CSO, Cellecta

10:20 – 10:30  Questions & Answers

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Thank you for your interest in Cellecta's educational event at 2018 ASCB/EMBO. Registration is closed.