Using Next-Generation Sequencing (NGS) Barcoding Technologies for Targeted Gene Expression Profiling and Single-Cell Genetic Analysis
Pooled lentiviral libraries of CRISPR sgRNA to mediate genome-wide gene knockout have become an invaluable tool for uncovering the functional genetic drivers required for a biological response. Another type of pooled lentiviral library designed with unique DNA sequence tags has been used to label large populations of cells with unique cell-specific barcodes which allows monitoring changes in sub-populations of cells with distinct phenotypes over time. Further, these barcode lentiviral libraries can be designed so that the unique barcode sequence is detectable in next-generation sequencing (NGS) RNA expression profiling assays (e.g., RNA-seq). As a result, investigators can analyze and link distinct molecular changes to sub-populations of cells with distinct gene expression profiles and phenotypes, such as drug resistance. The additional step of incorporating these cell barcodes together with genetic effectors, such as CRISPR sgRNA libraries, make it possible to tease out gene expression changes that result from specific genetic disruptions, and link these to the development of specific phenotypes. Cellecta will discuss work we are doing to develop this integrated platform that combines CRISPR sgRNA screens with single-cell genetic analysis.
Thank you for your interest in our Lunch-and-Learn event at ASHG.
Where: Room 28E, Upper Level, San Diego Convention Center
When: Thursday, October 18, 2018 –12:30 to 1:45 pm
12:30 pm Registration and Lunch
12:40 pm Introduction – Paul Diehl, Ph.D., COO, Cellecta
12:45 – 1:10 pm NGS Barcoding Technologies and Genome-Wide Targeted Expression Profiling for Single-Cell Genetic Analysis – Alex Chenchik, Ph.D., President & CSO, Cellecta
1:10 – 1:20 pm Quality RNAs used for CRISPR screening, Ben Borgo, Ph.D, Global Manager, Strategic Market Development, DGG, Agilent Technologies
1:20 – 1:45 pm Update: DriverMap Transcriptome Profiling in Drug Screening Applications – Lester Kobzik, M.D., Harvard School of Public Health, Harvard Medical School, Brigham & Womens Hospital
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