The CloneTracker™ 50M Lentiviral Barcode Library (formerly known as the CellTracker™ 50M Lentiviral Barcode Library and 13K × 13K Barcode Library) enables the tracking of individual clones derived from a population of cells. The pooled library contains more than 50 million lentiviral constructs with different barcode sequences. When a cell population is transduced with this barcode library, the barcodes integrate into the genomic DNA of the cells. The result is a select starter founder population where almost every cell has a different DNA-sequenceable barcode integrated into its DNA.
Since the barcode is stably integrated into the genome, it is passed on to any cell progeny when genomic DNA is replicated. This feature allows researchers to track cell proliferation over the course of an experiment. For instance, it is possible to:
- Track the fate of cell clones over a course of drug treatment.
- Study the effect of differentiation on hematopoietic cells.
- Assess the effect of treatment on implanted tumor cell populations.
The CloneTracker 50M Barcode Library is constructed in a Cellecta third-generation lentiviral vector that expresses both TagRFP (Evrogen) and a Puromycin resistance gene under a human Ubiquitin C promoter. The specially-designed, optimized barcodes facilitate Next-Generation Sequencing (NGS) data analysis and barcode identification. Using an Illumina NextSeq or HiSeq NGS instrument, barcode sequences are identified and converted to lists with enumerated data.
The protocols included provide guidelines for packaging the CloneTracker Barcode Library into VSV-G pseudotyped lentiviral particles, transduction of target cells, and the preparation of barcodes derived from transduced cells for NGS, enumeration, and analysis.
References and Product Citations for all Cellecta products can be found on the Cellecta website:
Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.
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