Use of the PromoterTest™ Assay Kit (cat.# PROMTEST) helps ensure the selection of a promoter suited to express a cDNA of interest in any mammalian cell system. RNA polymerase II promoters show a wide range of variation across different cells and different culture conditions. For experiments where strong expression of a gene is necessary, the PromoterTest Assay allows you to easily identify which of eight different RNA polymerase II promoter configurations in the Kit works most effectively in your cell line.
The PromoterTest Kit includes eight lentivectors prepackaged as ready-to-transduce lentiviral particles. Each lentivector carries the same green fluorescent protein (GFP) under the control of a different promoter. To use the kit, you simply need to transduce the lentivectors into your cell line, and compare the GFP intensities from each different construct.
The Kit provides enough reagents to test ~10 cell lines.
References and Product Citations for all Cellecta products can be found on the Cellecta website: https://www.cellecta.com/resources/publications/.
Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.
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