CellTracker™ Barcode Products
- Incorporate stable and heritable, sequenceable barcodes to label individual cells or cell populations
- Get individual barcode constructs and barcode libraries in selectable lentiviral constructs
- Use CRISPR-barcode library for Perturb-Seq/CRISPR-Seq screening
- Get uniquely identifiable barcode sequences even with several misreads
For researchers investigating tumor progression, drug resistance, differentiation, development, hematopoiesis, and other related areas, there is considerable interest in understanding the heterogeneity of cell populations in both cultured and in vivo settings. Studies facilitated by labeling cells with a stable heritable marker such as a CellTracker™ barcode include:
- how cell heterogeneity changes in response to drugs or other selections
- which traits allow cells to survive under different conditions
- how cell population diversity might change with differentiation or disease progression
Barcoded libraries, combined with next-generation sequencing (NGS), enable quantitative tracking of cell populations and clonal cell progeny. With these barcodes , cells can be permanently labeled and progeny tracked. Cell barcoding may be used to quantitatively assess representation of cells derived from a specifically barcode-tagged clone in a descendant population. For example, Cellecta and Amgen collaborated to use this sort of approach to assess the heterogeneity of tumors resulting from xenograft implantation. Based on this work, Cellecta developed its original CellTracker Lentiviral barcode library to introduce cell-specific unique barcodes into virtually any mammalian cell population.
Cellecta CellTracker™ Products:
Label millions of individual cells with unique sequenceable barcodes to track their proliferation and assess the diversity of their descendant populations in culture or in vivo.
More recently, groups have used libraries that express unique barcode sequences on mRNA in conjunction with single-cell RNA sequencing to identify cell sub-populations expressing distinct sets of genes containing differentiating cellular markers. For these single-cell expression analysis studies, the barcodes are positioned just before the poly-A tail of a marker or selection gene in the lentiviral library. When the library constructs integrate into the genomic DNA after transduction, the modified gene transcript is expressed with the barcode so the barcode can be detected in the cDNA generated using RNA-sequencing approaches. For this application, Cellecta has developed its CellTracker XP Libraries.
Label individual cells in a large population with unique expressed barcodes that can be detected by both genomic DNA sequencing and on cDNA generated during RNA sequencing.
An extension of using barcodes with RNA-Seq involves also incorporating an effector element, such as sgRNA, that perturbs cellular genomics by, for example, knocking out a gene. When this sort of element is included with the barcode, both can be detected with RNA-Seq and thus enable changes in gene expression to be correlated with specific disruptions or perturbations of particular genes. This provides a very powerful approach to identify the genes responsible for controlling expression of particular pathways or responses. For this application, Cellecta offers CellTracker XP™ CRISPR Libraries.
Human and mouse libraries incorporating expression of barcodes with gRNA sequences that are designed for CRISPR-Seq (aka Perturb-Seq) screens are also available.
Finally, Cellecta provides kits for labeling all cells in a particular population with a specific, single barcode. With these kits, 4-10 different cell populations can each be distinctly labeled, and the progeny from each cell population can be sampled after differently labeled populations are co-cultured or otherwise grown together.
Label whole cell populations with stable, genomically integrated, single identifiable sequenceable barcodes for cell tracking and quantification of descendants in mixed downstream cultures or tissue.
Convenient online ordering is available here.