CloneTracker XP™ Lentiviral Barcode Libraries
- Easily label cells with an RNA barcode readily detected with RNA-Seq analysis
- Choose from a range of lentiviral libraries with 100K, 1M, or 10M expressed barcodes
- Access multiple library pools so several cell populations can be labeled with uniquely discernible, non-overlapping barcodes
Single-cell RNA-seq analysis is an important tool to analyze individual cell responses. However, since all cell populations are phenotypically heterogenous, important genes and pathways may not be active in all cells. Thus, significant responses to experimental conditions that may only be present in a sub-population of the cells in any particular population may be easily missed as distinct. One way to address this challenge is to label individual cells with uniquely identifiable barcodes. In this way, effects and responses of each of the clonal progeny from this initial barcoded population can be easily assessed within study, and data correlating how experimental conditions may differentially affect distinct groups of cells from a common progenitor, can be extracted.
To detect cell-specific barcodes in conjunction with gene activation or pathway analysis, however, cell-specific barcodes need to be expressed on the mRNA. This can be done by incorporating the barcodes into the 3’- or 5’-UTR of an RNA transcript. Cellecta’s collection of ready-to-use barcode libraries enables convenient labeling of ca. 1 million individual cells with unique barcodes that are detected in both the genomic DNA and expressed RNA fractions. When used in conjunction with single-cell RNA-Seq analysis, the expressed barcode may be used to identify expression profiles and activated genes in different cells so that distinct clonal populations of cells from a single progenitor, as well as sub-populations of cells with distinct pathway activations or expression profiles, can be readily identified.
|BCXP10M5VP-P||CloneTracker XP 10M Barcode-5' Library with Venus-Puro (plasmid)||Barcode Libraries and Cell Labeling Kits||$8,500.00|| ||8500|
|BCXP10M5VP-V||CloneTracker XP 10M Barcode-5' Library with Venus-Puro (virus)||Barcode Libraries and Cell Labeling Kits||$5,000.00|| ||5000|