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CloneTracker™ 50M Lentiviral Barcode Library

Cellecta’s CloneTracker™ 50M Lentiviral Barcode Library (formerly CellTracker) enables the tracking of individual clones derived from a population of cells. The library contains more than 50 million lentiviral constructs with different barcode sequences. When a cell population is transduced with this barcode library, the barcodes integrate into the genomic DNA of the cells. The result is a select starter founder population where almost every cell has a different, heritable, DNA-sequenceable barcode integrated into its genomic DNA.

Since the barcode is stably integrated, it is passed onto any cell progeny when genomic DNA is replicated. This feature allows researchers to track cell proliferation over the course of an experiment. For instance, it is possible to:

  • Track the fate of cell clones over a course of drug treatment
  • Study the effect of differentiation on hematopoietic cells
  • Assess the effect of treatment on implanted tumor cell populations

Advantages of Barcodes with Precise, Known Sequences

  • Broad Applicability: VSV-g pseudotyped lentiviral particles efficiently transduce virtually any mammalian cells
  • Easy quantitation: Library contains 50 million barcodes designed to be easily sequenced with Illumina NextSeq, HiSeq, and GAIIx NGS platforms
  • Cell-tracking, simplified: Label as many as 1 million cells with unique barcodes in one experiment

Stable Barcode Integration to Permanently Label Cells

  • When transduced into cells, lentiviral vectors integrate into the host cell genome
  • As the host cells divide, the barcode sequences in the lentiviral vector will also replicate and be passed onto daughter cells
  • Cells can be treated, grown for several passages, frozen and thawed, and the sequences within the lentiviral vector will remain in the host cell
  • After selection, treatment, or differentiation, just extract genomic DNA, amplify, and sequence to identify and quantify barcodes present in the selected cell population

barcode distribution in CloneTracker 50M Barcode Library

Barcode distribution in CloneTracker™ 50M Barcode Library – Chart shows the number of times each barcode appeared in the library at an NGS sequencing depth of 45M reads. There are no reads that appeared more than 46 times, which is less than a 1 in a million frequency.

Ordering Information

BC13K-PCloneTracker™ 13K Lentiviral Barcode Library (plasmid)$6,500.00
BC13X13-PCloneTracker™ 50M Lentiviral Barcode Library (plasmid)$6,500.00
BC13K-VCloneTracker™ 13K Lentiviral Barcode Library (virus)$3,500.00
BC13X13-VCloneTracker™ 50M Lentiviral Barcode Library (virus)$3,500.00

A convenient Next-Generation Sequencing (NGS) Library Prep Kit (Catalog# LNGS-200), containing all the necessary primers specific to the CloneTracker Barcode Library, is also available to rapidly prepare the libraries for sequencing.

Please email with additional questions.

If interested in expressed barcode libraries so barcodes can be read by RNA-Seq, see our CloneTracker XP™ Barcode Libraries.

User Manual

  • CloneTracker™ 50M Lentiviral Barcode Library (previously CellTracker, 13K x 13K) (Online Manual or PDF)

Library Sequences

Vector Information

Product Certificates

Lot NumberCatalog NumberDescriptionQuantityProduct Certificate
181101003BC13X13-VCloneTracker™ 50M Lentiviral Barcode Library (virus)1 x 108 TUPDF
17060101BC13X13-VCloneTracker™ 50M Lentiviral Barcode Library (virus)1 x 108 TUPDF
17020201BC13K-VCloneTracker™ 13K Lentiviral Barcode Library (virus)1 x 108 TUPDF
12082901BC13X13-30M-P13Kx13K (CloneTracker 50M) Lentiviral Barcode Library (plasmid)200 µgPDF
12082103BC13K-PCloneTracker™ 13K Lentiviral Barcode Library (plasmid)200 µgPDF

Safety Data Sheets (SDS)

CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix$340.00
LNGS-200NGS Prep Kit for Barcode Libraries in pRSI16/17 (CloneTracker™)$875.00
LNGS-200-SPSupplementary Primer Set for LNGS-200 (12 Additional Index Primers)$475.00
LFVC1LentiFuge™ Viral Concentration Reagent$325.00
LTDR1LentiTrans™ Transduction Reagent (Polybrene for Transduction)$175.00
LTSET-GLentiPrep™ Lentiviral Reagent Set with GFP Control for Packaging and Transduction$750.00
LTSET-RLentiPrep™ Lentiviral Reagent Set with RFP Control for Packaging and Transduction$750.00

Next-Gen Sequencing and Analysis

CloneTracker Barcode Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the barcodes, sequence, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies barcodes
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerates barcode counts from raw sequencing data

Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.