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Cas9 Plasmids and Cells

Cellecta offers a range lentiviral constructs that express Streptococcus pyogenes (Sp) Cas9 nuclease or dCas9 (catalytically dead Cas9) hybrid genes for CRISPR knockout, CRISPRi, and CRISPRa gene editing and sgRNA screens.

The genes for all the Cas9 variations are over 4kb in size and thus are relatively large for lentiviral constructs. As a result, packaging of the Cas9 constructs is somewhat inefficient. Also, the expression level of the relatively large Cas9 proteins can vary significantly with different promoters in different cell systems and experiments.

Cas9 copy number and efficiency of knockout - CMV vs UbiC promoters

Data shows that higher levels of Cas9 expression increase the rate of gene knockout in cells. As a result, it makes sense to optimize the level of expression of Cas9 in cell models used for CRISPR gene editing. There are two straightforward ways to accomplish this for most cells:

1. Ensure that the expression level of the Cas9 gene is optimized for the target cells. For this purpose, we offer a range constructs with different promoters driving the Cas9 gene.

Cellecta also offers the PromoterTest Assay kit to facilitate testing and selection of an optimal promoter for specific target cells.

2. Maximize the MOI of infection of the Cas9 construct into the target cells. In doing this, it is possible to make cells that express more than one copy of the Cas9.

Cellecta offers the CRISPR-Test Assay kits to measure intercellular activity of Cas9 and the dCas9 activators or repressors.

Cas9 constructs are available in both packaged and plasmid forms.

Ordering information

Catalog#NameCategoryPriceBuy
SVC9-PSCRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (plasmid)$300.00
SVC9B-PSCRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (plasmid)$300.00
SVC9G-PSCRISPR Cas9 pRCCG-CMV-Cas9-2A-GFP (plasmid)$300.00
SVC9N-PSCRISPR Cas9 pRCCN-CMV-Cas9-2A-Neo (plasmid)$300.00
SVC9P-PSCRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (plasmid)$300.00
SVC9R-PSCRISPR Cas9 pRCCR-CMV-Cas9-2A-RFP (plasmid)$300.00
SVCOPG-PCRISPR Target for sgCopGFP Control (pRR-CMV-dscGFP-EF1-Bleo)$700.00
SVC9-VSCRISPR Cas9 pRCCH-CMV-Cas9-2A-Hygro (virus)$500.00
SVC9B-VSCRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (virus)$500.00
SVC9G-VSCRISPR Cas9 pRCCG-CMV-Cas9-2A-GFP (virus)$500.00
SVC9N-VSCRISPR Cas9 pRCCN-CMV-Cas9-2A-Neo (virus)$500.00
SVC9P-VSCRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (virus)$500.00
SVC9R-VSCRISPR Cas9 pRCCR-CMV-Cas9-2A-RFP (virus)$500.00
SVCOPG-VCRISPR Target for sgCopGFP Control (pRR-CMV-dscGFP-EF1-Bleo)$500.00

For use with CRISPR gene inhibition (CRISPRi) or gene activation (CRISPRa) systems, plasmids expressing either dCas9-KRAB or dCas9-VPH are available.

Catalog#NameCategoryPriceBuy
SVKRABC9B-PSCRISPRi dCas9-KRAB pRDKCCB-CMV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9H-PSCRISPRi dCas9-KRAB pRDKCCH-CMV-dCas9-KRAB-2A-Hygro (plasmid)$300.00
SVKRABC9B-VSCRISPRi dCas9-KRAB pRDKCCB-CMV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9H-VSCRISPRi dCas9-KRAB pRDKCCH-CMV-dCas9-KRAB-2A-Hygro (virus)$500.00
SVKRABC9E2B-PSCRISPRi dCas9-KRAB pRDKCE2B-EFS-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9E2B-VSCRISPRi dCas9-KRAB pRDKCE2B-EFS-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9MCB-PSCRISPRi dCas9-KRAB pRDKCMCB-mCMV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9MCB-VSCRISPRi dCas9-KRAB pRDKCMCB-mCMV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9RB-PSCRISPRi dCas9-KRAB pRDKCRB-RSV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9RB-VSCRISPRi dCas9-KRAB pRDKCRB-RSV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9SFB-PSCRISPRi dCas9-KRAB pRDKCSFB-SFFV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9SFB-VSCRISPRi dCas9-KRAB pRDKCSFB-SFFV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9ZCB-PSCRISPRi dCas9-KRAB pRDKCZCB-E-CMV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9ZCB-VSCRISPRi dCas9-KRAB pRDKCZCB-E-CMV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9ZE2B-PSCRISPRi dCas9-KRAB pRDKCZE2B-E-EFS-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9ZE2B-VSCRISPRi dCas9-KRAB pRDKCZE2B-E-EFS-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9ZRB-PSCRISPRi dCas9-KRAB pRDKCZRB-E-RSV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9ZRB-VSCRISPRi dCas9-KRAB pRDKCZRB-E-RSV-dCas9-KRAB-2A-Blast (virus)$500.00
Catalog#NameCategoryPriceBuy
SVVPHC9B-PSCRISPRa dCas9-VPH pRDVCCB-CMV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9B-VSCRISPRa dCas9-VPH pRDVCCB-CMV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9E2B-PSCRISPRa dCas9-VPH pRDVCE2B-EFS-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9E2B-VSCRISPRa dCas9-VPH pRDVCE2B-EFS-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9MCB-PSCRISPRa dCas9-VPH pRDVCMCB-mCMV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9MCB-VSCRISPRa dCas9-VPH pRDVCMCB-mCMV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9RB-PSCRISPRa dCas9-VPH pRDVCRB-RSV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9RB-VSCRISPRa dCas9-VPH pRDVCRB-RSV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9SFB-PSCRISPRa dCas9-VPH pRDVCSFB-SFFV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9SFB-VSCRISPRa dCas9-VPH pRDVCSFB-SFFV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9ZCB-PSCRISPRa dCas9-VPH pRDVCZCB-E-CMV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9ZCB-VSCRISPRa dCas9-VPH pRDVCZCB-E-CMV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9ZE2B-PSCRISPRa dCas9-VPH pRDVCZE2B-E-EFS-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9ZE2B-VSCRISPRa dCas9-VPH pRDVCZE2B-E-EFS-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9ZRB-PSCRISPRa dCas9-VPH pRDVCZRB-E-RSV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9ZRB-VSCRISPRa dCas9-VPH pRDVCZRB-E-RSV-dCas9-VPH-2A-Blast (virus)$500.00

We also offer MDA-MB-231 cells stably expressing Cas9, dCas9-KRAB or dCas9-VPH.

Catalog#NameCategoryPriceBuy
ZMDAMB231-C9Cas9+ MDA-MB-231 Cells, Blast selection$1,450.00
ZMDAMB231-CKRAMDA-MB-231 Stably expressing dCas9-KRAB$1,450.00
ZMDAMB231-CVPHMDA-MB-231 Cells Stably Expressing dCas9-VPH$1,450.00

We can also provide other configurations of Cas9 or dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.