Cas9 Plasmids and Cells
Cellecta offers a range lentiviral constructs that express Streptococcus pyogenes (Sp) Cas9 nuclease or dCas9 (catalytically dead Cas9) hybrid genes for CRISPR knockout, CRISPRi, and CRISPRa gene editing and sgRNA screens.
The genes for all the Cas9 variations are over 4kb in size and thus are relatively large for lentiviral constructs. As a result, packaging of the Cas9 constructs is somewhat inefficient. Also, the expression level of the relatively large Cas9 proteins can vary significantly with different promoters in different cell systems and experiments.
Data shows that higher levels of Cas9 expression increase the rate of gene knockout in cells. As a result, it makes sense to optimize the level of expression of Cas9 in cell models used for CRISPR gene editing. There are two straightforward ways to accomplish this for most cells:
1. Ensure that the expression level of the Cas9 gene is optimized for the target cells. For this purpose, we offer a range constructs with different promoters driving the Cas9 gene.
Cellecta also offers the PromoterTest Assay kit to facilitate testing and selection of an optimal promoter for specific target cells.
2. Maximize the MOI of infection of the Cas9 construct into the target cells. In doing this, it is possible to make cells that express more than one copy of the Cas9.
Cellecta offers the CRISPR-Test Assay kits to measure intercellular activity of Cas9 and the dCas9 activators or repressors.
Cas9 constructs are available in both packaged and plasmid forms.
For use with CRISPR gene inhibition (CRISPRi) or gene activation (CRISPRa) systems, plasmids expressing either dCas9-KRAB or dCas9-VPH are available.
We also offer MDA-MB-231 cells stably expressing Cas9, dCas9-KRAB or dCas9-VPH.
|ZMDAMB231-C9||Cas9+ MDA-MB-231 Cells, Blast selection||CRISPR Cas9||$1,450.00|| |
|ZMDAMB231-CKRA||MDA-MB-231 Stably expressing dCas9-KRAB||CRISPR Cas9||$1,450.00|| |
|ZMDAMB231-CVPH||MDA-MB-231 Cells Stably Expressing dCas9-VPH||CRISPR Cas9||$1,450.00|| |
We can also provide other configurations of Cas9 or dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.