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CRISPR Mouse Genome-Wide Knockout Library

Cellecta’s CRISPR Mouse Genome-Wide Knockout Library was designed to be small enough to enable convenient, genome-wide “dropout viability” screens. With fewer than 80,000 constructs, the mouse CRISPR library targets all mouse protein-coding genes (~19,600) with 4 sgRNAs/gene. It is designed to target functional domains and regions conserved between alternatively spliced transcripts.

Genome-Wide Coverage with a Single 80K Module

  • Single library with ~80,000 constructs
  • Each gene is covered by 4 sgRNAs designed to target functional domains and conserved transcript regions
  • All mouse protein-coding genes covered—approximately 19,600 gene targets
  • Convenient quantities available: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 packaged

Complete Panel of Controls

  • Standard non-targeting controls
  • Controls for non-specific cutting (sgRNA targeting introns throughout the genome)

If you are interested in a custom library, Cellecta can help. Visit our Custom Lentiviral Libraries page for specific instructions.

 

Ordering information

NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email us at orders@cellecta.com using your institutional email account, and ask for a quote with the discounted pricing.

We can also prepare custom libraries according to your specifications. Email sales@cellecta.com for details or to obtain a quote.

Quantity provided in each library: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 TU packaged
Catalog#NameQuantityPriceBuy
KOMGW-80K-PCRISPR Mouse Genome 80K Knockout Library (plasmid)200 ug$10,000.00
KOMGW-80K-V8CRISPR Mouse Genome 80K Knockout Library (virus)2 x 10^8 TU$8,500.00
KOMGW-80K-V9CRISPR Mouse Genome 80K Knockout Library (virus)1 x 10^9 TU$18,000.00

User Manual

Gene List

Vector Information

CRISPR Library VectorNGS CassetteSequence
(FASTA|GenBank|SnapGene*)
Map
pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-PuroHTS6C.txt | .gb | .dnaMap
pRSGEThy1.2-U6-sg-HTS6C-EF1-Thy1.2HTS6C.txt | .gb | .dnaMap

Product Certificates

Lot NumberCatalog NumberDescriptionQuantityProduct Certificate
181010002KOMGW-ET1.2-80K-V8CRISPR Mouse Genome 80K Knockout Library in Thy1.2 (virus)2 x 108 TUPDF
181010001KOMGW-ET1.2-80K-PCRISPR Mouse Genome 80K Knockout Library in Thy1.2 (plasmid)200 µgPDF
17101701KOMGW-80K-V8CRISPR Mouse Genome 80K Knockout Library (virus)2 x 108 TUPDF
17101101KOMGW-80K-PCRISPR Mouse Genome 80K Knockout Library (plasmid)200 µgPDF

Safety Data Sheets (SDS)

* Download the SnapGene® Viewer to open .dna format files.

Next-Gen Sequencing and Analysis

Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies sgRNA
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerates sgRNA counts from raw sequencing data

Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.

Cas9 Expression Plasmids

More information >

Catalog#NameQuantityPriceBuy
SVC9B-PSCRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (plasmid)25 ug$300.00
SVC9E2B-PSCRISPR Cas9 pRCE2B-EFS-Cas9-2A-Blast (plasmid)25 ug$300.00
SVC9G-PSCRISPR Cas9 pRCCG-CMV-Cas9-2A-GFP (plasmid)25 ug$300.00
SVC9N-PSCRISPR Cas9 pRCCN-CMV-Cas9-2A-Neo (plasmid)25 ug$300.00
SVC9P-PSCRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (plasmid)25 ug$300.00
SVC9R-PSCRISPR Cas9 pRCCR-CMV-Cas9-2A-RFP (plasmid)25 ug$300.00
SVC9RB-PSCRISPR Cas9 pRCRB-RSV-Cas9-2A-Blast (plasmid)25 ug$300.00
SVC9SFB-PSCRISPR Cas9 pRCSFB-SFFV-Cas9-2A-Blast (plasmid)25 ug$300.00
SVCOPG-PCRISPR Target for sgCopGFP Control (pRCdsGEB-CMV-dsCopGFP-EF1-Bleo, plasmid)25 ug$700.00
SVC9B-VSCRISPR Cas9 pRCCB-CMV-Cas9-2A-Blast (virus)1 x 10^6 TU$500.00
SVC9E2B-VSCRISPR Cas9 pRCE2B-EFS-Cas9-2A-Blast (virus)1 x 10^6 TU$500.00
SVC9G-VSCRISPR Cas9 pRCCG-CMV-Cas9-2A-GFP (virus)1 x 10^6 TU$500.00
SVC9N-VSCRISPR Cas9 pRCCN-CMV-Cas9-2A-Neo (virus)1 x 10^6 TU$500.00
SVC9P-VSCRISPR Cas9 pRCCP-CMV-Cas9-2A-Puro (virus)1 x 10^6 TU$500.00
SVC9R-VSCRISPR Cas9 pRCCR-CMV-Cas9-2A-RFP (virus)1 x 10^6 TU$500.00
SVC9RB-VSCRISPR Cas9 pRCRB-RSV-Cas9-2A-Blast (virus)1 x 10^6 TU$500.00
SVC9SFB-VSCRISPR Cas9 pRCSFB-SFFV-Cas9-2A-Blast (virus)1 x 10^6 TU$500.00
SVCOPG-VCRISPR Target for sgCopGFP Control (pRCdsGEB-CMV-dsCopGFP-EF1-Bleo, virus)1 x 10^7 TU$500.00

We can also provide other configurations of Cas9 or dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.

Catalog#NameQuantityPriceBuy
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix250 ug$340.00
LNGS-120NGS Prep Kit for sgRNA Libraries in pRSG16/17 (KOHGW) with Alignment SoftwareKit for 6-48 samples (48 preps of 50ug each, 12 multiplex)$950.00
LNGS-120-SPSupplementary Primer Set for LNGS-120 (12 Additional Index Primers)48 Reactions (12 Index Primers x 4 reactions each)$475.00
LFVC1LentiFuge™ Viral Concentration Reagent1 ml of 1000x$325.00
LTDR1LentiTrans™ Transduction Reagent (Polybrene for Transduction)1 ml of 1000x$175.00
LTSET-GLentiPrep™ Lentiviral Reagent Set with GFP Control for Packaging and TransductionKit$750.00
LTSET-RLentiPrep™ Lentiviral Reagent Set with RFP Control for Packaging and TransductionKit$750.00
CRUTESTCRISPRuTest™ Functional Cas9 Activity Assay Kit for Any Cell (Mammalian)1 kit (for 5-15 cell lines)$850.00
PROMTESTPromoterTest™ Assay Kit (8 promoter constructs)1 kit (for 10 cell lines)$895.00