CRISPR Mouse Genome-Wide Knockout Library
Cellecta’s CRISPR Mouse Genome-Wide Knockout Library was designed to be small enough to enable convenient, genome-wide “dropout viability” screens. With fewer than 80,000 constructs, the mouse CRISPR library targets all mouse protein-coding genes (~19,600) with 4 sgRNAs/gene. It is designed to target functional domains and regions conserved between alternatively spliced transcripts.
Genome-Wide Coverage with a Single 80K Module
- Single library with ~80,000 constructs
- Each gene is covered by 4 sgRNAs designed to target functional domains and conserved transcript regions
- All mouse protein-coding genes covered—approximately 19,600 gene targets
Complete Panel of Controls
- Standard non-targeting controls
- Controls for non-specific cutting (sgRNA targeting introns throughout the genome)
If you are interested in a custom library, Cellecta can help. Visit our Custom Lentiviral Libraries page for specific instructions.
NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email us at firstname.lastname@example.org using your institutional email account and ask for a quote with the discounted pricing.
- CRISPR Pooled Lentiviral sgRNA Libraries (Online Manual or PDF)
|CRISPR Library Vector||NGS Cassette||Sequence|
|pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-Puro||HTS6C||.txt | .gb | .dna||Map|
|pRSGEP-U6-sg-HTS6C-EF1-Puro||HTS6C||.txt | .gb | .dna||Map|
Lot-Specific Product Information (Product Analysis Certificates/PACs)
Safety Data Sheet (SDS)
* Download the SnapGene® Viewer to open .dna format files.
Next-Gen Sequencing and Analysis
Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.
You provide Cellecta with frozen cell pellets or tissue after screening…
…and Cellecta does the rest:
- Extracts genomic DNA
- Amplifies sgRNA
- Performs NGS on the Illumina NextSeq or HiSeq
- Enumerates sgRNA counts from raw sequencing data
Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.
|Catalog #||Item Description||Price|
|CPCP-K2A||Ready-to-Use Lentiviral Packaging Plasmid Mix, 250 μg|
(for (25) 10-cm plates)
|LFVC1||LentiFuge™ Viral Concentration Reagent, 1 ml|
(for 1 liter supernatant)
|CRUTEST||CRISPRuTest™ Functional Cas9 Activity Assay Kit (Mammalian)||$850|
|LNGS-120||NGS Prep Kit for KOHGW CRISPR sgRNA libraries||$875|
We can also prepare custom libraries according to your specifications. Email email@example.com for details or to obtain a quote.