CRISPR Mouse Genome-Wide Knockout Library
Cellecta’s CRISPR Mouse Genome-Wide Knockout Library was designed to be small enough to enable convenient, genome-wide “dropout viability” screens. With fewer than 80,000 constructs, the mouse CRISPR library targets all mouse protein-coding genes (~19,600) with 4 sgRNAs/gene. It is designed to target functional domains and regions conserved between alternatively spliced transcripts.
Genome-Wide Coverage with a Single 80K Module
- Single library with ~80,000 constructs
- Each gene is covered by 4 sgRNAs designed to target functional domains and conserved transcript regions
- All mouse protein-coding genes covered—approximately 19,600 gene targets
- Convenient quantities available: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 packaged
Complete Panel of Controls
- Standard non-targeting controls
- Controls for non-specific cutting (sgRNA targeting introns throughout the genome)
If you are interested in a custom library, Cellecta can help. Visit our Custom Lentiviral Libraries page for specific instructions.
NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email us at firstname.lastname@example.org using your institutional email account, and ask for a quote with the discounted pricing.
We can also prepare custom libraries according to your specifications. Email email@example.com for details or to obtain a quote.
Quantity provided in each library: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 TU packaged
- CRISPR Pooled Lentiviral sgRNA Libraries (Online Manual or PDF)
|CRISPR Library Vector||NGS Cassette||Sequence|
|pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-Puro||HTS6C||.txt | .gb | .dna||Map|
|pRSGEP-U6-sg-HTS6C-EF1-Puro||HTS6C||.txt | .gb | .dna||Map|
|Lot Number||Catalog Number||Description||Quantity||Product Certificate|
|181010002||KOMGW-ET1.2-80K-V8||CRISPR Mouse Genome 80K Knockout Library in Thy1.2 (virus)||2 x 108 TU|
|181010001||KOMGW-ET1.2-80K-P||CRISPR Mouse Genome 80K Knockout Library in Thy1.2 (plasmid)||200 µg|
|17101701||KOMGW-80K-V8||CRISPR Mouse Genome 80K Knockout Library (virus)||2 x 108 TU|
|17101101||KOMGW-80K-P||CRISPR Mouse Genome 80K Knockout Library (plasmid)||200 µg|
Safety Data Sheets (SDS)
* Download the SnapGene® Viewer to open .dna format files.
Next-Gen Sequencing and Analysis
Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.
You provide Cellecta with frozen cell pellets or tissue after screening…
…and Cellecta does the rest:
- Extracts genomic DNA
- Amplifies sgRNA
- Performs NGS on the Illumina NextSeq or HiSeq
- Enumerates sgRNA counts from raw sequencing data
Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.
Cas9 Expression Plasmids
We can also provide other configurations of Cas9 or dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.