Single-Module Human Genome-Wide CRISPR Library (80K)
Cellecta’s Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we have proven to generate more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.
Genome-Wide Coverage with a Convenient Single 80K Module
- Single library with ~80,000 constructs targeting approximately 19,000 protein-coding genes
- Each gene is targeted by 4 sgRNAs designed based on Doench, et al. criteria
- Targets all human protein-coding genes—over 19,000 gene targets.
The 80K Single-Module Human Genome-Wide CRISPR Library includes the following controls
- standard non-targeting controls
- non-specific control (sgRNA targeting introns throughout the genome)
- lethal sgRNA targeting generally essential genes (i.e., positive controls for dropout screens)
NOTE: Cellecta offers academic discounts on many of our pre-made libraries to qualifying academic institutions. Please email firstname.lastname@example.org using your institutional email account, and ask for a quote with the academic discount pricing.
For information on custom libraries, email email@example.com
The target gene list for the Single-Module Human Genome-Wide CRISPR Knockout Library can be downloaded here:
|CRISPR Library Vector||NGS Cassette||Sequence (.txt | .gb | .dna*)||Map|
|pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-Puro||HTS6C||FASTA | GenBank | SnapGene||Map|
|pRSGEP-U6-sg-HTS6C-EF1-Puro||HTS6C||FASTA | GenBank | SnapGene||Map|
* Download the SnapGene® Viewer to open .dna format files.
|Catalog #||Item Description||Price|
|CPCP-K2A||Ready-to-Use Lentiviral Packaging Plasmid Mix, 250 μg|
(for (25) 10-cm plates)
|LFVC1||LentiFuge™ Viral Concentration Reagent, 1 ml|
(for 1 liter supernatant)
|CRUTEST||CRISPRuTest™ Functional Cas9 Activity Assay Kit (Mammalian)||$850|
|LNGS-120||NGS Prep Kit for KOHGW CRISPR sgRNA libraries||$875|
We can also prepare custom libraries according to your specifications. Email firstname.lastname@example.org for details or to obtain a quote.