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Single-Module Human Genome-Wide CRISPR Library (80K)

Cellecta’s Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we have proven to generate more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.

Genome-Wide Coverage with a Convenient Single 80K Module

  • Single library with ~80,000 constructs targeting approximately 19,000 protein-coding genes
  • Each gene is targeted by 4 sgRNAs designed based on Doench, et al. criteria
  • Targets all human protein-coding genes—over 19,000 gene targets.

The 80K Single-Module Human Genome-Wide CRISPR Library includes the following controls

  • standard non-targeting controls
  • non-specific control (sgRNA targeting introns throughout the genome)
  • lethal sgRNA targeting generally essential genes (i.e., positive controls for dropout screens)

Ordering information

NOTE: Cellecta offers academic discounts on many of our pre-made libraries to qualifying academic institutions. Please email orders@cellecta.com using your institutional email account, and ask for a quote with the academic discount pricing.

Catalog#NameCategoryPriceBuyprice_hsort
KOHGW-80K-PCRISPR Human Genome 80K Knockout Library (plasmid)$10,000.00
10000
KOHGW-80K-V8CRISPR Human Genome 80K Knockout Library (virus)$8,500.00
8500
KOHGW-80K-V9CRISPR Human Genome 80K Knockout Library (virus)$18,000.00
18000
KOHGW-EP-80K-PCRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (plasmid)$10,000.00
10000
KOHGW-EP-80K-V8CRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (virus)$8,500.00
8500
KOHGW-EP-80K-V9CRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (virus)$18,000.00
18000

For information on custom libraries, email sales@cellecta.com

 

Single-module Human Genome-Wide CRISPR Library
Single-module Human Genome-Wide CRISPR Library Kit image
Library Representation - Human 80K
Z-scores of sgRNA handle designs

User Manual

Product Analysis Certificates (PACs) for pre-made CRISPR sgRNA libraries are provided as PDF files and are available for download on the Product Manuals and Certificates page under the Resources section.

Gene List

The target gene list for the Single-Module Human Genome-Wide CRISPR Knockout Library can be downloaded here:

Vector Information

CRISPR Library VectorNGS CassetteSequence (.txt | .gb | .dna*)Map
pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-PuroHTS6CFASTA | GenBank | SnapGeneMap
pRSGEP-U6-sg-HTS6C-EF1-PuroHTS6CFASTA | GenBank | SnapGeneMap

* Download the SnapGene® Viewer to open .dna format files.

Catalog #Item DescriptionPrice
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix, 250 μg
(for (25) 10-cm plates)
$325
LFVC1LentiFuge™ Viral Concentration Reagent, 1 ml
(for 1 liter supernatant)
$325
CRUTESTCRISPRuTest™ Functional Cas9 Activity Assay Kit (Mammalian)$850
LNGS-120NGS Prep Kit for KOHGW CRISPR sgRNA libraries $875

We can also prepare custom libraries according to your specifications. Email sales@cellecta.com for details or to obtain a quote.