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Custom Libraries for Barcoding Cells

In addition to custom CRISPR sgRNA and RNAi shRNA libraries, Cellecta can provide custom barcode libraries for cell labeling. We also offer CloneTracker™ Lentiviral Barcode Libraries as off-the-shelf products.

However, some studies may require specialized barcode libraries:

  • Limited complexity–a small number of defined barcodes. Our off-the-shelf libraries with 1M, 10M, and 50M barcodes may be too complex for your experimental needs. Some studies looking at population distributions may only require a few thousand barcodes. We can certainly provide smaller libraries with 1000, 5000 or 20,000 unique, distinct barcodes. With these libraries of limited complexity, deeper sequencing of the whole library is possible.
  • Specialized markers or selection. For some barcode tracking experiments, a different marker or selection may be required, such as Thy1.1, luciferase, or thymidine kinase. We can readily generate a barcode library of any size in a specialized vector for you.
  • Barcoding combined with other effectors or elements in a library. As deep sequencing has become much more accessible, barcode tracking has become a useful tool for tracking cell-to-cell variations in conjunction with a variety of assays. For example, several groups have focused on using barcode tracking to assay RNA expression in conjunction with pooled CRISPR screening (see blog post). For this application, Cellecta has introduced its Scribe Vector Series so that we can create CRISPR (and RNAi) libraries with transcribed barcodes that are trackable in RNA-Seq assays.
Example pRSGScribe vectors for custom barcode libraries expressing sgRNA

There are many examples where the power of barcode tracking can provide the resolution necessary to understand how cell-to-cell differences affect biological responses. Cellecta’s expertise in pooled library construction and barcode development can provide you with the tools to help you understand these studies.

Example in vivo mouse application using a custom barcode library with 27,000 barcodes

If you are interested in a specialized custom barcode library, please contact us to discuss your needs and obtain a quote.