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Engineered Custom Cell Lines

As part of its extensive functional genomics portfolio, Cellecta offers custom cell line engineering services.

  • Knockout (CRISPR KO)
  • Knock-in (CRISPRa)
  • Knockdown (RNAi)
  • Overexpressor (cDNA)
  • Reporter cell lines

Lentiviral technology enables efficient generation of stable cell lines starting from almost any mammalian cell. The chromosomally-integrated lentiviral cassette provides a long-term stable expression that is less likely to perturb the cellular biology than episomal constructs.

Cellecta’s flexible system can be used to make combinations of knockout, knockdown, expressor, and reporter lines in virtually any type of mammalian cell, including primary cells. Cell lines (pools and clonal cell lines) are sequence-verified. Choose your gene of interest and cell background, and Cellecta will do the rest, providing you with a validated cell line.

Cellecta isogenic cell line engineering

Engineered Cell Line Applications include

  • Gene function analysis
  • Compound screening
  • Understanding disease models and gene-drug association

A range of stable clones can easily be generated and then selected for complete knockout or optimum knockdown, knock-in, expression, or activation, depending on the experimental requirements. An almost unlimited number of stable clones can be produced, enabling selection of the clones with the best characteristics for experimental assays. Find more information on our lentiviral vector system in the Technology section.

Engineered Cell Line ServicesFormatDeliverables
CRISPR Knockout CellsCell PoolStable cell pool, sequence verified
CRISPR Knockout CellsClonalGenerate clonal cell lines, Assess knockout in (all) alleles. Expand clone(s) of choice.
RNAi Knockdown CellsCell PoolStable cell pool with knockdown data for gene(s) of interest
RNAi Knockdown CellsClonalGenerate clonal cell lines, measure shRNA knockdown activity by qRT-PCR. Expand clone(s) of choice.
CRISPR Knock-in CellsCell PoolStable cell pool with knock-in data for gene(s) of interest
CRISPR Knock-in CellsClonalGenerate clonal cell lines, Assess knock-in in (all) alleles. Expand clone(s) of choice.
cDNA Overexpression CellsCell PoolStable cell pool, sequence verified
cDNA Overexpression CellsClonalGenerate clonal cell lines, measure mRNA and/or protein expression. Expand clone(s) of choice.
Transcriptional Reporter CellsCell PoolStable cell pool, sequence verified
Transcriptional Reporter CellsClonalGenerate clonal cell lines, measure transcriptional activity by functional test. Expand clone(s) of choice.

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