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Custom Engineered Cell Lines

Cellecta has the technology and expertise to engineer a variety of cell lines for specific experimental needs. We provide knockout, knock-in, knockdown, protein expression, CRISPR-Cas9 expression, and reporter cell lines for use in various applications:

Cellecta stable cell line engineering

  • Reporter cell lines for compound screens
  • Isogenic knockout cell lines for CRISPR or RNAi screens
  • Expression cell lines to analyze gene function and gene-drug association
  • Validated Cas9 expression cell lines for targeted CRISPR knockout or sgRNA screening

 

Depending on the specific needs of the project, Cellecta uses a variety of techniques to knockout, insert, mutate, or otherwise introduce genetic modifications to a parental cell line. For instance, we extensively make use of lentiviral technology to efficiently and permanently introduce genetic elements into genomic DNA. For knockout cell lines, however, CRISPR sgRNA and Cas9 can be transiently introduced into cells using episomal constructs to generate the knockout, then they are lost during propagation so the only permanent alternation is disruption of the target gene.

The following pages offer more information on the services we provide to make cells with the following specific modifications:

Interested in a customized cell line?

Contact us and let us know what you need. Each project is unique. Depending on the cell lines and particular genetic changes, the price will vary. However, we would be happy to set up a time to discuss what you need with our expert cell biologists and go over the options we could offer.