Custom CRISPR Cas9 Cells
CRISPR knockout requires expression of both a single-guide RNA (sgRNA) that targets the specific site for knockout, and expression of the Cas9 nuclease which cuts the DNA at the target site. Also, for studies using the CRISPRa or CRISPRi systems, cells need to express the corresponding dCas9 activator (e.g. dCas9-VPH or dCas9-VPR) or dCas9-repressor (e.g. dCas9-KRAB). For many CRISPR applications then, it is useful or necessary to have cells expressing the Cas9 protein.
In addition to offering a range of Cas9 expressing plasmids, including dCas9-KRAB and dCas9-VPH constructs for CRISPRa and CRISPRi, Cellecta also provides a service where we make validated Cas9+ expression cells for CRISPR knockout, CRISPRi and CRISPRa applications.
For our standard service, we transduce your parental cell line of choice with the appropriate Cas9-lentiviral construct, and then expand under selection to produce a population of Cas9-expressing cells. Finally, we measure the activity of the Cas9 variant in the selected cell population with one of our CRISPRtest Assays. We typically do this at a few MOIs to provide you with a healthy culture with the highest Cas9 activity.
To order or to learn more, please contact us.