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Custom Reporter Cell Lines

Transcription factor-dependent reporter cell lines can be ideal tools for high-throughput RNAi, peptide, and chemical library screens. Responsive reporter cells form the core of cell-based assays for screening and identifying drugs, antibodies, cell factors, and other effectors. With lentiviral-based transduction, Cellecta can easily engineer stable reporter lines for almost any application–regardless of cell type.

Stable Reporter Cell Lines

  • Cell lines can be made from nearly any cell type, including those that are difficult to transfect
  • Reporter is integrated in the natural chromosomal environment creating a stable cell line that maintains responsiveness
  • Effectors and transactivating elements that might be required for reporter function can be accommodated
  • Available reporters include GFP, RFP, PuroR, BleoR, or other readout markers
  • We provide you with a validated, stable reporter cell line
  • Clonal selection also available
  • Service takes about 3 months

Cellecta’s Lentiviral Reporter Constructs are lentiviral vectors containing a reporter under the control of a minimum (m)CMV promoter and a transcriptional response element (RE). In the example on the right, the NFkB TRE is located upstream of mCMV, and the pair of elements becomes a fully active promoter and able to express dZsGreen  when bound  by the specific transcriptional factor (TF).

For more information or to place an order, please contact us.

Reporter Cell Line Activation
Reporter Cell Line Activation