Single Vector CRISPR/Cas9 System
The Single Vector CRISPR system can be used for knockout of individual genes in vivo or in vitro by expressing both sgRNA (single guide RNA or gRNA) and Cas9 nuclease from the same lentiviral vector.
Stable Expression of Cas9 and sgRNA
- Co-transduction not necessary since sgRNA and Cas9 nuclease are expressed in one vector
- Lentiviral vectors integrate into the host cell genome and are passed onto daughter cells
- Cells can be treated, grown for several passages, frozen, and thawed
- The lentiviral construct remains in the host cells
- Vector contains antibiotic selection to ensure stability
Permanently Knock Out Targets with Lentiviral sgRNA Constructs
- You provide the RefSeq number or Gene ID
- We can design 2-5 sgRNA constructs that target the gene
- Grassian AR, Scales TM, Knutson SK, Kuntz KW, McCarthy NJ, Lowe CE, Moore JD, Copeland RA, Keilhack H, Smith JJ, Wickenden JA, Ribich S. A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality. Biol Proced Online. 2015 Nov 14;17:15. eCollection 2015. PubMed PMID: 26578851; PubMed Central PMCID: PMC4647477.
To place an order or simply calculate pricing, please fill out the Single Vector CRISPR Construct Order Form and email it to us at email@example.com. Please indicate whether you are placing an order or requesting a quote. If placing an order, please refer to the PO number and email the PO to firstname.lastname@example.org or fax it to 650-938-3911.
For our selection of positive and negative CRISPR control constructs, please see our Control Constructs web page.
|Catalog #||Item Description||Price|
|CPCP-K2A||Ready-to-Use Lentiviral Packaging Plasmid Mix, 250 μg|
(for (25) 10-cm plates)
|LFVC1||LentiFuge™ Viral Concentration Reagent, 1 ml|
(for 1 liter supernatant)
|CRTEST||CRISPRtest™ Functional Cas9 Activity Kit (for 5 cell line pairs)||$750|