LOCUS Cellecta-pRCDCME 7609 bp ds-DNA circular SYN 12-OCT-2017 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7609) AUTHORS Cellecta TITLE Direct Submission JOURNAL Exported Oct 12, 2017 from SnapGene 4.0.6 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|668563200| COMMENT VNTDBDATE|819417600| COMMENT VNTNAME|Cellecta-pRCDCMEP-CMV-MCS-EF1-Puro.dna| COMMENT VNTAUTHORNAME|Cellecta| FEATURES Location/Qualifiers promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2220..2446 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2447..2627 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2674..2799 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3292..3525 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4015..4132 /label=cPPT /note="central polypurine tract and central termination sequence of HIV-1" promoter 4139..4482 /label=CMV /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 4485..4503 /label=MCS promoter 4506..5049 /label=EF1 CDS 5071..5670 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" misc_feature 5677..6265 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6341..6574 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6646..6767 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 6807..6942 /label=SV40 ori /note="SV40 origin of replication" rep_origin 7152..7607 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt 2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct 2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact 2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt 2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa 2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa 2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca 3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata 3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag 3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa 3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcctcaa tgacgctgac 3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 3601 tctggaacag attggaatca cacgacctgg atggagtggg acagagaaat taacaattac 3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttttaa 4021 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 4081 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgaaat 4141 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4201 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4261 gactcacggg gatttccaag tctccacccc attgacgcca atgggagttt gttttggcac 4321 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4381 ggtaggcgtg tacggtggaa ggtttatata agcagagctc gtttagtgaa ccgtcagatc 4441 gcctggagac gccatccacg ctgttttgac ctccatagaa gattctagag tgaattcttt 4501 tcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 4561 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 4621 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 4681 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 4741 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 4801 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 4861 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 4921 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 4981 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 5041 ggcgcctacc ccgggaagct agacgccacc atgaccgagt acaagcccac ggtgcgcctc 5101 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5161 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5221 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5281 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5341 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5401 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5461 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5521 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5581 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5641 tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt 5701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6061 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 6121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 6241 atctcccttt gggccgcctc cccgcctggg tacctttaag accaatgact tacaaggcag 6301 ctgtagatct tagccacttt ttaaaagaaa aggggggact tggaagggct aattcactcc 6361 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 6421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6541 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6601 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 6661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 6721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 6781 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 6841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 6961 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 7021 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 7081 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 7141 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 7201 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 7261 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 7321 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 7381 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 7441 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 7501 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 7561 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcc //