LOCUS       Cellecta-pRCEH-E       11746 bp ds-DNA     circular SYN 23-JAN-2018
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Cellecta
  TITLE     Direct Submission
  JOURNAL   Exported Jan 23, 2018 from SnapGene 4.1.4 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|728265600|
COMMENT     VNTDBDATE|831427200|
COMMENT     VNTNAME|Cellecta-pRCEH-EF1-Cas9-2A-Hygro.dna|
COMMENT     VNTAUTHORNAME|Cellecta|
FEATURES             Location/Qualifiers
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1818..1933
                     /label=cPPT
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2010..2523
                     /label=EF1
     CDS             2542..2562
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             2566..2586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             2590..2610
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             2617..6717
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
     CDS             6718..6765
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
     CDS             6781..6834
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             6841..7812
                     /codon_start=1
                     /label=HygroR
     misc_feature    7825..8413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8487..8720
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8792..8913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8953..9088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9523..10111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10282..11142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(11143..11247)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 ttgcttggat cgagcaattt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1921 aaattcaaaa tttctgcgtt gttgtcggtg ctcgttctct gctcttcacg ctactgaatt
     1981 catcaccggt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2041 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     2101 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2161 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2221 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     2281 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     2341 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     2401 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     2461 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     2521 tactctagag ccaccatggc cccaaaaaag aagagaaagg tagatccaaa aaagaagaga
     2581 aaggtagatc caaaaaagaa gagaaaggta ggtggagaca agaagtacag catcggcctg
     2641 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     2701 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     2761 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     2821 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     2881 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     2941 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3001 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3061 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3121 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3181 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     3241 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     3301 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     3361 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     3421 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     3481 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     3541 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     3601 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     3661 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     3721 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     3781 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     3841 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     3901 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     3961 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4021 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4081 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4141 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4201 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     4261 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     4321 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     4381 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     4441 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     4501 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     4561 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     4621 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     4681 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     4741 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     4801 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     4861 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     4921 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     4981 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5041 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5101 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5161 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     5221 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     5281 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     5341 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     5401 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     5461 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     5521 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     5581 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     5641 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     5701 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     5761 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     5821 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     5881 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     5941 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6001 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6061 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6121 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6181 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     6241 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     6301 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     6361 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     6421 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     6481 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     6541 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     6601 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     6661 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaaa
     6721 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaaggcggc cgcaggatgc
     6781 gagggcagag gaagccttct aacatgcggt gacgtggagg agaatcccgg cccttccggg
     6841 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6901 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6961 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     7021 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     7081 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     7141 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     7201 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     7261 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     7321 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     7381 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     7441 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7501 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     7561 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     7621 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     7681 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     7741 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7801 tgtgtagaag tctgataagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7861 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7921 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7981 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8041 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8101 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8161 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8221 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8281 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8341 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctctctttgg
     8401 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8461 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8521 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8701 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8761 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8881 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9001 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9061 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9121 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9181 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9241 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10561 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11281 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11341 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11401 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11461 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11521 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11581 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11641 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11701 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
