LOCUS Exported 11240 bp ds-DNA circular SYN 02-NOV-2016 DEFINITION . ACCESSION . VERSION . KEYWORDS Cellecta-pRDKCCB-CMV-dCas9-KRAB-2A-Blast SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11240) AUTHORS Sylvain Baron TITLE Direct Submission JOURNAL Exported Nov 2, 2016 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11240 /organism="synthetic DNA construct" /mol_type="other DNA" source 2357..2362 /organism="recombinant plasmid" /mol_type="other DNA" /note="color: #ffffff" promoter 6..232 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" /note="color: #ffffff; direction: RIGHT" LTR 233..413 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 460..585 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1078..1311 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature 1818..1933 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /note="color: #ffe4c4" promoter 2005..2348 /note="CMV" /note="color: #ffffff; direction: RIGHT" misc_feature 2357..2362 /note="Kozak" /note="color: #a6acb3" CDS 2369..2389 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2393..2413 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2417..2437 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2444..6544 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /note="This feature has 5 segments: 1: 2444 .. 2467 / #993366 2: 2468 .. 2470 / #cc99b2 3: 2471 .. 4957 / #993366 4: 4958 .. 4960 / #cc99b2 5: 4961 .. 6544 / #993366" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6545..6592 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /note="nucleoplasmin NLS" /note="color: #993366" /translation="KRPAATKKAGQAKKKK" CDS 6602..6826 /codon_start=1 /gene="ZNF10" /gene_synonym="KOX1" /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /note="KRAB" /note="color: #993366" /translation="MDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLE NYKNLVSLGYQLTKPDVILRLEKGEEPWLV" CDS 6851..6904 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 6901" /translation="EGRGSLLTCGDVEENPGP" CDS 6911..7309 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /note="Blast (BSD)" /note="confers resistance to blasticidin" /note="color: #ccffcc" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 7319..7907 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" /note="color: #ffffff" LTR 7981..8214 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 8286..8407 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin 8447..8582 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" rep_origin complement(9017..9605) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(9776..10636) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 9776 .. 10567 / #ccffcc 2: 10568 .. 10636 / #ccffcc / signal sequence Cleavage site after base 10567" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10637..10741) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 ttgcttggat cgagcaattt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa 1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa 1921 aaattcaaaa tttctgcgtt gttgtcggtg ctcgttctct gctcttcacg ctactgaatt 1981 catcaccggt tcttcgaatc tagtattatg cccagtacat gaccttatgg gactttccta 2041 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2101 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2161 acgccaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2221 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtggaaggtt tatataagca 2281 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 2341 atagaagatt ctagccgcca ccatggcccc aaaaaagaag agaaaggtag atccaaaaaa 2401 gaagagaaag gtagatccaa aaaagaagag aaaggtaggt ggagacaaga agtacagcat 2461 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 2521 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 2581 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac 2641 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 2701 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 2761 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 2821 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 2881 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 2941 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 3001 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 3061 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 3121 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 3181 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 3241 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 3301 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 3361 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 3421 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 3481 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc 3541 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 3601 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 3661 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 3721 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 3781 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 3841 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag 3901 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 3961 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt 4021 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc 4081 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 4141 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 4201 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 4261 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 4321 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 4381 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 4441 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 4501 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 4561 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 4621 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 4681 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 4741 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 4801 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 4861 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 4921 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct 4981 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag 5041 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct 5101 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg 5161 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 5221 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga 5281 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 5341 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 5401 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 5461 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 5521 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 5581 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 5641 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct 5701 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 5761 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga 5821 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 5881 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 5941 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 6001 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 6061 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 6121 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 6181 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac actacctgga 6241 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 6301 ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag agcaggccga 6361 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 6421 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 6481 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 6541 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggcggccga 6601 aatggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca aggatgtatt 6661 tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga tcgtgtacag 6721 aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc ttactaagcc 6781 agatgtgatc ctccggttgg agaagggaga agagccctgg ctggtggaga gagaagcggc 6841 cgcaggatgc gagggcagag gaagccttct aacatgcggt gacgtggagg agaatcccgg 6901 cccttccggg atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac 6961 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc 7021 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc 7081 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt 7141 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct 7201 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc 7261 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaat aagtcgacaa 7321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 7381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 7441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 7501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 7561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 7621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 7681 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 7741 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 7801 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 7861 ccttcgccct cagacgagtc ggatctctct ttgggccgcc tccccgcctg gtacctttaa 7921 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 7981 tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc 8041 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 8101 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 8161 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 8221 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 8281 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8401 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 8461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 8521 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 8581 cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt 8641 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 8701 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 8761 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 8821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 8881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 8941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 9001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 9061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 9241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 9301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 9361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 9481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 9661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 9721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 9841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 9901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 9961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 10021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 10081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 10141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 10201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 10261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 10321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 10381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 10441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 10501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 10561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 10621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 10681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 10741 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 10801 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 10861 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 10921 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 10981 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 11041 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 11101 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 11161 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 11221 cgacggccag tgccaagctg //