LOCUS       Cellecta-pRDKCCH       11816 bp ds-DNA     circular SYN 30-SEP-2017
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Cellecta
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2017 from SnapGene 4.0.5 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|818208000|
COMMENT     VNTDBDATE|818208000|
COMMENT     VNTNAME|Cellecta-pRDKCCH-CMV-dCas9-KRAB-2A-Hygro.dna|
COMMENT     VNTAUTHORNAME|Cellecta|
FEATURES             Location/Qualifiers
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1818..1933
                     /label=cPPT
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2005..2348
                     /label=CMV
     misc_feature    2357..2362
                     /label=Kozak
     CDS             2369..2389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2393..2413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2417..2437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2444..6544
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10 mutation in the 
                     RuvC-like domain and the H840A mutation in the HNH domain"
     CDS             6545..6592
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
     CDS             6602..6826
                     /codon_start=1
                     /gene="ZNF10"
                     /gene_synonym="KOX1"
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
     CDS             6851..6904
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             6911..7882
                     /codon_start=1
                     /label=HygroR
     misc_feature    7895..8483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8557..8790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8862..8983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9023..9158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9593..10181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10352..11212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(11213..11317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 ttgcttggat cgagcaattt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1921 aaattcaaaa tttctgcgtt gttgtcggtg ctcgttctct gctcttcacg ctactgaatt
     1981 catcaccggt tcttcgaatc tagtattatg cccagtacat gaccttatgg gactttccta
     2041 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2101 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2161 acgccaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2221 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtggaaggtt tatataagca
     2281 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2341 atagaagatt ctagccgcca ccatggcccc aaaaaagaag agaaaggtag atccaaaaaa
     2401 gaagagaaag gtagatccaa aaaagaagag aaaggtaggt ggagacaaga agtacagcat
     2461 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
     2521 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
     2581 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac
     2641 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
     2701 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
     2761 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
     2821 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     2881 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     2941 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     3001 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     3061 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
     3121 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
     3181 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     3241 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     3301 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     3361 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     3421 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     3481 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc
     3541 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     3601 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     3661 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     3721 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     3781 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     3841 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag
     3901 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     3961 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt
     4021 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc
     4081 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     4141 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     4201 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     4261 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     4321 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     4381 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     4441 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     4501 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     4561 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     4621 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     4681 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     4741 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     4801 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     4861 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     4921 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct
     4981 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag
     5041 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct
     5101 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg
     5161 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     5221 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga
     5281 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     5341 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     5401 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     5461 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     5521 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     5581 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     5641 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct
     5701 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     5761 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga
     5821 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     5881 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     5941 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     6001 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     6061 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     6121 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     6181 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac actacctgga
     6241 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     6301 ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag agcaggccga
     6361 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     6421 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     6481 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     6541 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggcggccga
     6601 aatggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca aggatgtatt
     6661 tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga tcgtgtacag
     6721 aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc ttactaagcc
     6781 agatgtgatc ctccggttgg agaagggaga agagccctgg ctggtggaga gagaagcggc
     6841 cgcaggatgc gagggcagag gaagccttct aacatgcggt gacgtggagg agaatcccgg
     6901 cccttccggg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     6961 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     7021 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7081 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7141 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7201 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7261 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7321 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7381 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7441 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7501 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7561 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7621 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     7681 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     7741 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     7801 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     7861 gaccgatggc tgtgtagaag tctgataagt cgacaatcaa cctctggatt acaaaatttg
     7921 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8281 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8401 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     8461 ctctctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     8521 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8581 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8641 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8701 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8761 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8821 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8881 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8941 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     9001 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9121 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat
     9181 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9241 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9301 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9361 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10021 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10321 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10381 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10441 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10501 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10561 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10621 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10681 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10741 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10801 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10861 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10921 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10981 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11041 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11101 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11161 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11221 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11281 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11341 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    11401 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    11461 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    11521 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    11581 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    11641 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    11701 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    11761 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
