LOCUS Exported 12164 bp ds-DNA circular SYN 02-NOV-2016 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS Cellecta-pRDVCCB-CMV-dCas9-VPH-2A-Blast SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12164) AUTHORS Donato TITLE Direct Submission JOURNAL Exported Nov 2, 2016 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12164 /organism="recombinant plasmid" /mol_type="other DNA" /note="color: #ffffff" promoter 6..232 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" /note="color: #ffffff; direction: RIGHT" LTR 233..413 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 460..585 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1078..1311 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature 1818..1933 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /note="color: #ffe4c4" promoter 2005..2348 /label=CMV /note="CMV" /note="color: #ffffff; direction: RIGHT" misc_feature 2357..2362 /note="Kozak" /note="color: #a6acb3" CDS 2369..2389 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2393..2413 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2417..2437 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 2444..6544 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /note="This feature has 5 segments: 1: 2444 .. 2467 / #993366 2: 2468 .. 2470 / #cc99b2 3: 2471 .. 4957 / #993366 4: 4958 .. 4960 / #cc99b2 5: 4961 .. 6544 / #993366" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6545..6592 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /note="nucleoplasmin NLS" /note="color: #993366" /translation="KRPAATKKAGQAKKKK" CDS 6620..6769 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /note="VP64" /note="color: #993366" /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 6872..7267 /codon_start=1 /note="p65" /note="color: #993366" /translation="LAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDA DEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVT GSQRPPDPAPTPLGTSGLPNGLSGDEDF" CDS 7343..7714 /codon_start=1 /note="HSF1" /note="color: #993366" /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED PTISLLTGSEPPKAKDPTVS" CDS 7730..7750 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #993366" /translation="PKKKRKV" CDS 7775..7828 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 7825" /translation="EGRGSLLTCGDVEENPGP" CDS 7835..8233 /codon_start=1 /note="Blast (BSD)" /note="color: #ccffcc" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 8243..8831 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" /note="color: #ffffff" LTR 8905..9138 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 9210..9331 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin 9371..9506 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" rep_origin complement(9941..10529) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(10700..11560) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 10700 .. 11491 / #ccffcc 2: 11492 .. 11560 / #ccffcc / signal sequence Cleavage site after base 11491" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(11561..11665) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 ttgcttggat cgagcaattt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa 1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa 1921 aaattcaaaa tttctgcgtt gttgtcggtg ctcgttctct gctcttcacg ctactgaatt 1981 catcaccggt tcttcgaatc tagtattatg cccagtacat gaccttatgg gactttccta 2041 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2101 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2161 acgccaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2221 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtggaaggtt tatataagca 2281 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 2341 atagaagatt ctagccgcca ccatggcccc aaaaaagaag agaaaggtag atccaaaaaa 2401 gaagagaaag gtagatccaa aaaagaagag aaaggtaggt ggagacaaga agtacagcat 2461 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 2521 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 2581 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac 2641 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 2701 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 2761 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 2821 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 2881 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 2941 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 3001 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 3061 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 3121 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 3181 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 3241 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 3301 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 3361 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 3421 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 3481 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc 3541 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 3601 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 3661 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 3721 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 3781 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 3841 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag 3901 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 3961 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt 4021 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc 4081 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 4141 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 4201 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 4261 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 4321 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 4381 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 4441 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 4501 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 4561 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 4621 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 4681 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 4741 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 4801 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 4861 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 4921 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct 4981 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag 5041 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct 5101 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg 5161 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 5221 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga 5281 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 5341 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 5401 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 5461 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 5521 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 5581 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 5641 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct 5701 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 5761 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga 5821 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 5881 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 5941 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 6001 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 6061 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 6121 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 6181 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac actacctgga 6241 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 6301 ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag agcaggccga 6361 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 6421 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 6481 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 6541 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggttccgg 6601 aggaggaggt agcggtagtg atgctttaga cgattttgac ttagatatgc ttggttcaga 6661 cgcgttagac gacttcgacc tagacatgtt aggctcagat gcattggacg acttcgattt 6721 agatatgttg ggctccgatg ccctagatga ctttgattta gatatgctag gtagtccttc 6781 agggcagatc agcaaccagg ccctggctct ggcccctagc tccgctccag tgctggccca 6841 gactatggtg ccctctagtg ctatggtgcc tctggcccag ccacctgctc cagcccctgt 6901 gctgacccca ggaccacccc agtcactgag cgctccagtg cccaagtcta cacaggccgg 6961 cgaggggact ctgagtgaag ctctgctgca cctgcagttc gacgctgatg aggacctggg 7021 agctctgctg gggaacagca ccgatcccgg agtgttcaca gatctggcct ccgtggacaa 7081 ctctgagttt cagcagctgc tgaatcaggg cgtgtccatg tctcatagta cagccgaacc 7141 aatgctgatg gagtaccccg aagccattac ccggctggtg accggcagcc agcggccccc 7201 cgaccccgct ccaactcccc tgggaaccag cggcctgcct aatgggctgt ccggagatga 7261 agacttctca agcatcgctg atatggactt tagtgccctg ctgtcacaga tttcctctag 7321 tgggcaggga ggaggtggaa gcggcttcag cgtggacacc agtgccctgc tggacctgtt 7381 cagcccctcg gtgaccgtgc ccgacatgag cctgcctgac cttgacagca gcctggccag 7441 tatccaagag ctcctgtctc cccaggagcc ccccaggcct cccgaggcag agaacagcag 7501 cccggattca gggaagcagc tggtgcacta cacagcgcag ccgctgttcc tgctggaccc 7561 cggctccgtg gacaccggga gcaacgacct gccggtgctg tttgagctgg gagagggctc 7621 ctacttctcc gaaggggacg gcttcgccga ggaccccacc atctccctgc tgacaggctc 7681 ggagcctccc aaagccaagg accccactgt ctccggatcc ggcagtggac ctaagaaaaa 7741 gaggaaggtg ggaggatctg cggccgcagg atgcgagggc agaggaagcc ttctaacatg 7801 cggtgacgtg gaggagaatc ccggcccttc cgggatggcc aagcctttgt ctcaagaaga 7861 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta 7921 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata 7981 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc 8041 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc 8101 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa 8161 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt 8221 gtgggagggc taataagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac 8281 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 8341 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 8401 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 8461 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 8521 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 8581 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 8641 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 8701 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 8761 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ctctttgggc 8821 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 8881 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc 8941 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 9001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 9061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 9121 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 9181 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 9241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 9301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 9361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 9421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 9481 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt 9541 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 9601 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 9661 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 9721 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 9781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 9841 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 9901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 9961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 10021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 10081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 10141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 10201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 10261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 10321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 10381 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 10441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 10501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 10561 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 10621 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 10681 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 10741 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 10801 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 10861 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 10921 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 10981 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 11041 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 11101 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 11161 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 11221 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 11281 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 11341 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 11401 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 11461 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 11521 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 11581 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 11641 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 11701 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 11761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 11821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 11881 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 11941 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 12001 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 12061 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 12121 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg //