LOCUS       Cellecta-pRSGCCR       11613 bp ds-DNA     circular SYN 26-JUN-2017
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Sylvain Baron
  TITLE     Direct Submission
  JOURNAL   Exported Jun 26, 2017 from SnapGene 2.7.3 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|758592000|
COMMENT     VNTDBDATE|809568000|
COMMENT     VNTNAME|Cellecta-pRSGCCR-U6-sg-HTS6C-CMV-Cas9-2A-TagRFP|
COMMENT     VNTAUTHORNAME|Sylvain Baron|
FEATURES             Location/Qualifiers
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        1819..2059
                     /label=U6
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2065..2094
                     /label=sgRNA cloning site
     misc_feature    2122..2237
                     /label=cPPT
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2309..2652
                     /label=CMV
     CDS             2673..2693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2697..2717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2721..2741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2748..6848
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
     CDS             6849..6896
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
     CDS             6912..6965
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             6972..7682
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
     misc_feature    7692..8280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8354..8587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8659..8780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8820..8955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9390..9978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10149..11009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(11010..11114)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt
     2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg
     2281 aattcatcac cggttcttcg aatctagtat tatgcccagt acatgacctt atgggacttt
     2341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2461 attgacgcca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggaa ggtttatata
     2581 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2641 ctccatagaa gattctagcc gccaccatgg ccccaaaaaa gaagagaaag gtagatccaa
     2701 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggtggagac aagaagtaca
     2761 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2821 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2881 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2941 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     3001 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     3061 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3121 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3181 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3241 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3301 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3361 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3421 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3481 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3541 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3601 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3661 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3721 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3781 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3841 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3901 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3961 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     4021 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     4081 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4141 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4201 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4261 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4321 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4381 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4441 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4501 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4561 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4621 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4681 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4741 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4801 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4861 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4921 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4981 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     5041 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     5101 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5161 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5221 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     5281 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5341 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5401 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5461 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5521 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5581 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5641 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5701 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5761 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5821 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5881 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5941 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     6001 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     6061 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6121 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6181 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6241 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6301 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6361 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6421 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6481 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6541 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6601 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6661 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6721 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6781 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6841 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggcgg
     6901 ccgcaggatg cgagggcaga ggaagccttc taacatgcgg tgacgtggag gagaatcccg
     6961 gcccttccgg aatggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
     7021 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     7081 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     7141 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
     7201 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     7261 tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     7321 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     7381 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     7441 gcctggaagg cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca
     7501 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     7561 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     7621 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     7681 attaggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7741 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7801 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7861 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7921 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7981 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     8041 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8101 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8161 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8221 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc tctttgggcc gcctccccgc
     8281 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8341 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     8401 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8461 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8521 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8581 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8641 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8761 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8881 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8941 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt
     9001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9061 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9181 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9241 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9301 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9361 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9421 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9481 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9541 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9601 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9661 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9721 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9781 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9841 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9901 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9961 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10021 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10081 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10141 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10201 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10261 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10321 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10381 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10441 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10501 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10561 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10621 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10681 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10741 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10801 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10861 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10921 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10981 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11041 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11101 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11161 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11221 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11281 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11341 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    11401 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    11461 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11521 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11581 cgacgttgta aaacgacggc cagtgccaag ctg
//
