LOCUS       Cellecta-pRSGCCT       11379 bp ds-DNA     circular SYN 06-MAR-2019
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Cellecta
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 6, 2019 from SnapGene 4.3.4 to Vector NTI(R)
            format
            https://www.snapgene.com
COMMENT     HTSC Cassette
COMMENT     VNTDATE|692150400|
COMMENT     VNTDBDATE|871430400|
COMMENT     VNTNAME|Cellecta-pRSGCCThy-U6-sg-HTS6C-CMV-Cas9-2A-Thy1.1.dna|
COMMENT     VNTAUTHORNAME|Cellecta|
FEATURES             Location/Qualifiers
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        1819..2059
                     /label=U6
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2065..2094
                     /label=sgRNA cloning site
     misc_feature    2122..2237
                     /label=cPPT
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2309..2652
                     /label=CMV
     CDS             2673..2693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2697..2717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2721..2741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2748..6848
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
     CDS             6849..6896
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
     CDS             6909..6962
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             6963..7451
                     /codon_start=1
                     /label=Thy1.1
     misc_feature    7458..8046
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8120..8353
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8425..8546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8586..8721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9156..9744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9915..10775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(10776..10880)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt
     2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg
     2281 aattcatcac cggttcttcg aatctagtat tatgcccagt acatgacctt atgggacttt
     2341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2461 attgacgcca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggaa ggtttatata
     2581 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2641 ctccatagaa gattctagcc gccaccatgg ccccaaaaaa gaagagaaag gtagatccaa
     2701 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggtggagac aagaagtaca
     2761 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2821 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2881 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2941 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     3001 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     3061 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3121 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3181 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3241 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3301 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3361 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3421 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3481 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3541 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3601 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3661 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3721 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3781 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3841 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3901 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3961 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     4021 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     4081 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4141 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4201 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4261 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4321 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4381 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4441 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4501 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4561 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4621 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4681 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4741 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4801 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4861 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4921 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4981 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     5041 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     5101 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5161 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5221 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     5281 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5341 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5401 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5461 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5521 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5581 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5641 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5701 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5761 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5821 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5881 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5941 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     6001 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     6061 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6121 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6181 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6241 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6301 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6361 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6421 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6481 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6541 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6601 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6661 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6721 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6781 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6841 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggcgg
     6901 ccgcaggaga gggcagagga agccttctaa catgcggtga cgtggaggag aatcccggcc
     6961 ctatgaaccc agccatcagc gtcgctctcc tgctctcagt cttgcaggtg tcccgagggc
     7021 agaaggtgac cagcctgaca gcctgcctgg tgaaccaaaa ccttcgcctg gactgccgcc
     7081 atgagaataa caccaaggat aactccatcc agcatgagtt cagcctgacc cgagagaaga
     7141 ggaagcacgt gctctcaggc accctcggga tacccgagca cacgtaccgc tcccgcgtca
     7201 ccctctccaa ccagccctat atcaaggtcc ttaccctagc caacttcacc accaaggatg
     7261 agggcgacta cttttgtgag cttcgagtct cgggcgcgaa tcccatgagc tccaataaaa
     7321 gtatcagtgt gtatagagac aaactggtca agtgtggcgg cataagcctg ctggttcaga
     7381 acacatcctg gatgctgctg ctgctgcttt ccctctccct cctccaagcc ctggacttca
     7441 tttctctgtg agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7501 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7561 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7621 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7681 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7741 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7801 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7861 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7921 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7981 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctctctt tgggccgcct
     8041 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8101 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     8161 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8221 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8281 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8341 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8401 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8461 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8521 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8581 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8641 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8701 gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag
     8761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    11041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    11101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11161 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    11221 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11281 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11341 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
