LOCUS       Cellecta-pRSGCEG       11789 bp ds-DNA     circular SYN 26-APR-2018
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Cellecta
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2018 from SnapGene 4.1.9 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     HTSC Cassette
COMMENT     VNTDATE|703036800|
COMMENT     VNTDBDATE|839980800|
COMMENT     VNTNAME|Cellecta-pRSGCEG-U6-sg-HTS6C-EF1-Cas9-2A-TagGFP2.dna|
COMMENT     VNTAUTHORNAME|Cellecta|
FEATURES             Location/Qualifiers
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        1819..2059
                     /label=U6
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2065..2094
                     /label=cloning site
     misc_feature    2122..2237
                     /label=cPPT
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2314..2806
                     /label=EF1
     CDS             2846..2866
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2870..2890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2894..2914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
     CDS             2921..7021
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
     CDS             7022..7069
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
     CDS             7085..7138
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             7145..7858
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
     misc_feature    7868..8456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8530..8763
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8835..8956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8996..9131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(9566..10154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10325..11185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(11186..11290)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt
     2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg
     2281 aattcatcac cggtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2341 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     2401 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     2461 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     2521 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     2581 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     2641 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     2701 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     2761 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     2821 cgcctactct agagccacca tggccccaaa aaagaagaga aaggtagatc caaaaaagaa
     2881 gagaaaggta gatccaaaaa agaagagaaa ggtaggtgga gacaagaagt acagcatcgg
     2941 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     3001 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     3061 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     3121 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     3181 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     3241 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     3301 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3361 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3421 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3481 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     3541 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     3601 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     3661 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     3721 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     3781 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     3841 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     3901 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     3961 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     4021 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     4081 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     4141 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     4201 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     4261 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     4321 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     4381 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4441 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     4501 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     4561 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     4621 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     4681 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     4741 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     4801 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     4861 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     4921 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     4981 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     5041 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     5101 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     5161 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     5221 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     5281 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     5341 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5401 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     5461 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     5521 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     5581 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     5641 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     5701 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     5761 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     5821 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     5881 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     5941 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     6001 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     6061 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     6121 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     6181 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     6241 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     6301 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     6361 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6421 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6481 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     6541 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     6601 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     6661 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     6721 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     6781 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     6841 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     6901 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     6961 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     7021 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg cggccgcagg
     7081 atgcgagggc agaggaagcc ttctaacatg cggtgacgtg gaggagaatc ccggcccttc
     7141 cggaatgagc gggggcgagg agctgttcgc cggcatcgtg cccgtgctga tcgagctgga
     7201 cggcgacgtg cacggccaca agttcagcgt gcgcggcgag ggcgagggcg acgccgacta
     7261 cggcaagctg gagatcaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     7321 cctggtgacc accctctgct acggcatcca gtgcttcgcc cgctaccccg agcacatgaa
     7381 gatgaacgac ttcttcaaga gcgccatgcc cgagggctac atccaggagc gcaccatcca
     7441 gttccaggac gacggcaagt acaagacccg cggcgaggtg aagttcgagg gcgacaccct
     7501 ggtgaaccgc atcgagctga agggcaagga cttcaaggag gacggcaaca tcctgggcca
     7561 caagctggag tacagcttca acagccacaa cgtgtacatc cgccccgaca aggccaacaa
     7621 cggcctggag gctaacttca agacccgcca caacatcgag ggcggcggcg tgcagctggc
     7681 cgaccactac cagaccaacg tgcccctggg cgacggcccc gtgctgatcc ccatcaacca
     7741 ctacctgagc actcagacca agatcagcaa ggaccgcaac gaggcccgcg accacatggt
     7801 gctcctggag tccttcagcg cctgctgcca cacccacggc atggacgagc tgtacaggtc
     7861 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     8041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     8101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     8281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8401 gcgtctccgc cttcgccctc agacgagtcg gatctctctt tgggccgcct ccccgcctgg
     8461 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8521 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     8581 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8641 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8701 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8761 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8821 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8881 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8941 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     9001 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9061 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9121 ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg
     9181 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9241 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9301 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9361 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10621 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10921 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    11341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    11401 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    11461 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    11521 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    11581 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    11641 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    11701 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    11761 gttgtaaaac gacggccagt gccaagctg
//
