LOCUS Cellecta-pRSIUTh 7136 bp ds-DNA circular SYN 11-AUG-2017 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7136) AUTHORS Peter TITLE Direct Submission JOURNAL Exported Aug 11, 2017 from SnapGene 2.7.3 to Vector NTI(R) format http://www.snapgene.com COMMENT This file is best viewed with a free Vector NTI Viewer: ORIGDB|GenBank COMMENT VNTDATE|309916800| COMMENT VNTDBDATE|813801600| COMMENT VNTNAME|Cellecta-pRSIUThy-U6-sh-HTS6-UbiC-Thy1.1| COMMENT VNTAUTHORNAME|Peter| FEATURES Location/Qualifiers promoter 6..232 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 233..413 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 460..585 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1078..1311 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." promoter 1819..2059 /label=U6 /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 2065..2094 /label=cloning site misc_feature 2122..2237 /label=cPPT /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2303..2702 /label=UbiC /note="human ubiquitin C promoter" CDS 2714..3202 /codon_start=1 /label=Thy1.1 misc_feature 3215..3803 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 3877..4110 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4182..4303 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 4343..4478 /label=SV40 ori /note="SV40 origin of replication" rep_origin complement(4913..5501) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5672..6532) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(6533..6637) /gene="bla" /label=AmpR promoter ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga 1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta 1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt 2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg 2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg 2281 aattcatcac cggttcttcg aaggcctccg cgccgggttt tggcgcctcc cgcgggcgcc 2341 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct 2401 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag 2461 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt 2521 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct 2581 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt 2641 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg 2701 gtctagagcc accatgaacc cagccatcag cgtcgctctc ctgctctcag tcttgcaggt 2761 gtcccgaggg cagaaggtga ccagcctgac agcctgcctg gtgaaccaaa accttcgcct 2821 ggactgccgc catgagaata acaccaagga taactccatc cagcatgagt tcagcctgac 2881 ccgagagaag aggaagcacg tgctctcagg caccctcggg atacccgagc acacgtaccg 2941 ctcccgcgtc accctctcca accagcccta tatcaaggtc cttaccctag ccaacttcac 3001 caccaaggat gagggcgact acttttgtga gcttcgagtc tcgggcgcga atcccatgag 3061 ctccaataaa agtatcagtg tgtatagaga caaactggtc aagtgtggcg gcataagcct 3121 gctggttcag aacacatcct ggatgctgct gctgctgctt tccctctccc tcctccaagc 3181 cctggacttc atttctctgt gaatctaggt cgacaatcaa cctctggatt acaaaatttg 3241 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 3301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 3361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 3421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 3481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 3541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 3601 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 3661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 3721 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 3781 ctctctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 3841 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 3901 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 3961 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 4021 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 4081 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 4141 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 4201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 4321 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4441 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat 4501 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 5521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 5881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 5941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 6001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 6061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6661 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 6721 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 6781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 6841 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 6901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 6961 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 7021 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 7081 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg //