CellTrackerTM Barcode Cell Labeling Kits
Pre-packaged lentiviral constructs with unique easily identifiable DNA sequences that stably integrate into the genomic DNA of any mammalian target cells. The kits contain sufficient pre-packaged lentiviral particles of each barcode to generate starter populations of millions of cells with the same stably integrated sequence, that can be easily retrieved by PCR and read by sequencing. Labeled populations can then later be mixed or co-cultured with other either unlabeled or differently labeled cells. After several generations, cells from each specific population can be identified and quantified by amplifying and sequencing the unique barcode sequence.
CellTracker 4-Barcode-Plus Cell Labeling Kit
Designed with 4 genomically integratable barcodes that, not only can be differentiated by sequencing, but also by PCR and qPCR. In addition to having a unique sequence, common primer sequences for each barcode amplify a different size product so populations labeled with a specific barcode can be identified on an agarose gel. Further, each barcode has a unique qPCR probe sequence that enables differential amplification, and quantitation using qPCR.
Each kit contains 10^7TU of 4 individual pre-packaged lentiviral particles to label millions of cells. The barcode constructs in this kit are selectable by puromycin and do not contain a fluorescent marker.
|BC4P-V||CellTracker 4-Barcode-plus Cell Labeling Kit||10^7TU/construct (4)||$3,200|
CellTracker 5-Barcode and 10-Barcode Cell Labeling Kits
Each kit contains 5 or 10 genomically integratable barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and selection into cells, barcodes can be amplified and sequenced to identify estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then later the representative numbers of descendent cells from each population can be determined.
Each kit contains 10^7TU of either 5 or 10 individual pre-packaged lentiviral particles to label millions of cells. The barcode constructs in this kit are selectable by puromycin and also have a red fluorescent protein marker so cells containing barcodes will fluoresce red.
|BCRPP5-V||CellTracker 5-Barcode Cell Labeling Kit||10^7TU/construct (5)||$2,500|
|BCRPP10-V||CellTracker 10-Barcode Cell Labeling Kit||10^7TU/construct (10)||$5,000|