CloneTracker XP™ Lentiviral Expressed Barcode Libraries
- Easily label cells with an RNA expressed barcode readily detected with RNA-Seq analysis
- Choose from lentiviral libraries with 1 million or 10 million expressed barcodes
- Access up to 10 unique 1M barcode library pools so different cell populations can be labeled with uniquely discernible, non-overlapping, expressed barcode sequences
Single-cell RNA-Seq analysis is an important tool to analyze individual cell responses. However, since all cell populations are phenotypically heterogeneous, important genes and pathways may not be active in all cells. Thus, significant responses to experimental conditions that may only be present in a sub-population of the cells in any particular population may be easily missed as distinct. One way to address this challenge is to label individual cells with uniquely identifiable, expressed barcodes. In this way, effects and responses of each of the clonal progeny from this initial barcoded population can be easily assessed within the study, and data correlating how experimental conditions may differentially affect distinct groups of cells from a common progenitor can be extracted.
To detect cell-specific barcodes in conjunction with gene activation or pathway analysis, however, barcodes need to be expressed on the mRNA. This can be done by incorporating the barcodes into the 3′- or 5′-UTR of an RNA transcript. Cellecta’s collection of ready-to-use expressed barcode libraries enables convenient labeling of up to 1 million individual cells with unique barcodes that are detected in both the genomic DNA and expressed RNA fractions. When used in conjunction with single-cell RNA-Seq analysis, the expressed barcodes may be used to identify expression profiles and activated genes in different cells so that distinct clonal populations of cells from a single progenitor, as well as subpopulations of cells with distinct pathway activations or expression profiles, can be readily identified.
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Cellecta also provides Custom Barcode Libraries if one of our off-the-shelf designs does not meet your needs.
- Provided with purchase of library
Lot#-Specific Product Information (Product Analysis Certificates/PACs)
- CloneTracker XP 1M Barcode-3′ Library Pool 1 with RFP-Puro (Virus):
- CloneTracker XP 10M Barcode-3′ Library with Venus-Puro (Virus):
Safety Data Sheet (SDS)
NGS Prep Kit for barcode library sequencing