CloneTracker™ Barcode Products
Incorporate stable, heritable, and sequenceable barcodes in individual cells or cell populations
For researchers investigating tumor progression, drug resistance, differentiation, development, hematopoiesis, and other related areas, there is considerable interest in understanding the heterogeneity of cell populations in both cultured and in vivo settings. Studies facilitated by labeling cells with CloneTracker barcode products include:
- how cell heterogeneity changes in response to drugs or other selections
- which traits allow cells to survive under different conditions
- how cell population diversity might change with differentiation or disease progression
Track Cell Populations and Progeny
CloneTracker Barcode Libraries, combined with Next-Generation Sequencing (NGS), enable quantitative tracking of cell populations and clonal cell progeny. With these barcodes, cells can be permanently labeled and progeny tracked. Cell barcoding may be used to quantitatively assess representation of cells derived from a specifically barcode-tagged clone in a descendant population.
For example, Cellecta and Amgen collaborated to use this sort of approach to assessing the heterogeneity of tumors resulting from xenograft implantation. Based on this work, Cellecta developed its original CloneTracker Lentiviral Barcode Library to introduce cell-specific unique barcodes into virtually any mammalian cell population.
Cellecta CloneTracker™ Products
Label whole cell populations with stable, genomically integrated, single identifiable, sequenceable barcodes for cell tracking and quantification of descendants in mixed downstream cultures or tissue. Library constructs integrate into the genomic DNA after transduction and can be detected by genomic DNA amplification and, with the CloneTracker XP™ libraries, in RNA sequencing analysis. Sub-libraries with defined barcodes are available to label several cell populations with distinct non-overlapping barcodes.
Cellecta CloneTracker XP-rLuc libraries have the same design as the other CloneTracker XP™ libraries, with a barcode inserted in the 3′-UTR of the reporter transcript so it is detectable by RNA sequencing. Rather than a fluorescent protein marker, CloneTracker XP-rLuc libraries have a red-shifted luciferase (rLuc) derived from Photinus pyralis (Ppy RE9 mutant) as a reporter for bioluminescent imaging (BI).
Human and mouse libraries incorporating expression of barcodes with gRNA sequences that are designed for CRISPR-Seq (aka Perturb-Seq) screens are also available. In cells transduced with these libraries, researchers can detect both the clonal barcodes and the sgRNA effector with RNA Sequencing so that changes in gene expression can be correlated with specific disruptions of particular genes.
Cellecta provides kits for labeling all cells in a particular population with a specific, single barcode. With these kits, 4-10 different cell populations can each be distinctly labeled, and the progeny from each cell population can be sampled after differently labeled populations are co-cultured or otherwise grown together.
Convenient online ordering is available here.
- Quantitative clonal barcode analysis for the identification of key drivers of disease progression and drug resistance
- Combining Cell Barcoding and CRISPR sgRNA Libraries with Targeted Expression for Single-Cell Genetic Analysis of Tumor Development (April 2019)