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CRISPRa and CRISPRi Human Genome-Wide Libraries

The original CRISPR-Cas9 complex enables highly targeted knockout of essentially any gene but variations of the CRISPR system use engineered versions of the deactivated Cas9 (dCas9) protein to either interfere (i.e., repress) or activate gene expression without modifying genomic DNA. These gene-regulating variations of CRISPR use the same basic sgRNA design but target gene promoter regions rather than the coding sequence. The promoter-targeting sgRNA, then, recruits a modified dCas9 protein which either activates or represses gene expression—depending on the dCas9 modification. Cellecta’s CRISPRa and CRISPRi Human Genome-Wide sgRNA Libraries have been designed and optimized for pooled activator and repressor genetic screens.

Pooled screens can be run with both the CRISPR-activation (CRISPRa) and CRISPR-interference (CRISPRi) systems. Cellecta’s CRISPRa and CRISPRi human genome-wide sgRNA libraries have been designed with the following features to facilitate these screens:

  • Single-Module libraries–each expressing ~106,000 sgRNA
  • Each library targets almost 19,000 human protein-coding genes
  • Each gene is targeted by 5 sgRNAs designed against the promoter region (designs based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554)
  • 3,700 non-targeting sgRNA controls

 

Human Genome-Wide CRISPRa sgRNA Library

Selective activation of targeted genes when used in cells expressing dCas9-VPH (or other dCas9-activator variations compatible with spCas9 sgRNA designs)

Human Genome-Wide CRISPRi sgRNA Library

Selective expression inhibition of targeted genes when used in cells expressing dCas9-KRAB (or other deactivated Cas9 repressors compatible with spCas9 sgRNA designs)

 

Mouse Genome-Wide CRISPRa and CRISPRi libraries are also available.

Selective expression inhibition of targeted genes when used in cells expressing dCas9-KRAB (or other deactivated Cas9 repressors compatible with spCas9 sgRNA designs)

 

Ordering information

NOTE: Cellecta offers academic discounts on many of our pre-packaged libraries to qualifying academic institutions. Please email orders@cellecta.com using your institutional email account, and ask for a quote with the discounted pricing.

Catalog#NameCategoryPriceBuy
KAHGW-106-PHuman Genome-Wide CRISPRa sgRNA Library (plasmid)$10,000.00
KAHGW-106-V8Human Genome-Wide CRISPRa sgRNA Library (virus)$8,500.00
KAHGW-106-V9Human Genome-Wide CRISPRa sgRNA Library (virus)$18,000.00
Catalog#NameCategoryPriceBuy
KIHGW-106-PHuman Genome-Wide CRISPRi sgRNA Library (plasmid)$10,000.00
KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)$8,500.00
KIHGW-106-V9Human Genome-Wide CRISPRi sgRNA Library (virus)$18,000.00

If you are interested in a custom library, Cellecta can help. Visit our Order page for specific instructions.

User Manual

Gene Lists

sgRNA Sequences

  • Sequences are provided upon shipment of library.

Vector Information

CRISPR Library VectorNGS CassetteSequence
(FASTA|GenBank|SnapGene*)
Map
pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-PuroHTS6C.txt | .gb | .dnaMap

Product Certificates

Lot NumberCatalog NumberDescriptionQuantityProduct Certificate
181128001KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)2 x 108 TUPDF
180124001KIHGW-106-PHuman Genome-Wide CRISPRi sgRNA Library (plasmid)200 µgPDF
18011730KAHGW-106-V8Human Genome-Wide CRISPRa sgRNA Library (virus)2 x 108 TUPDF
18011702KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)2 x 108 TUPDF
17122701KAHGW-106-PHuman Genome-Wide CRISPRa sgRNA Library (plasmid)200 µgPDF

 

Safety Data Sheets (SDS)

Next-Gen Sequencing and Analysis

Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies sgRNA
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerates sgRNA counts from raw sequencing data

Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.

dCas9 Expression Plasmids

More information >

Catalog#NameCategoryPriceBuy
SVKRABC9-PS-plsamidCRISPRa dCas9-KRAB (For use with the CRISPR gene inhibition) - plasmid$300.00
SVKRABC9B-VS-virusCRISPRa dCas9-KRAB (For use with the CRISPR gene inhibition) - virus$500.00

Catalog#NameCategoryPriceBuy
SVVPHC9B-PS-plasCRISPRa dCas9-VPH (For use with the CRISPR gene activation) - plasmid$300.00
SVVPHC9B-VS-virusCRISPRa dCas9-VPH (For use with the CRISPR gene activation) - virus$500.00

We can also provide other configurations of dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.

Catalog #Item DescriptionPrice
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix, 250 μg
(for (25) 10-cm plates)
$340
LFVC1LentiFuge™ Viral Concentration Reagent, 1 ml
(for 1 liter supernatant)
$325
CRATESTCRISPRaTest™ Functional dCas9-Activator Assay Kit$850
CRITESTCRISPRiTest™ Functional dCas9-Repressor Assay Kit$850
LNGS-120NGS Prep Kit for KOHGW CRISPR sgRNA libraries $875