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CRISPRa and CRISPRi Human Genome-Wide Libraries

The original CRISPR-Cas9 complex enables highly targeted knockout of essentially any gene but variations of the CRISPR system use engineered versions of the deactivated Cas9 (dCas9) protein to either interfere (i.e., repress) or activate gene expression without modifying genomic DNA. These gene-regulating variations of CRISPR use the same basic sgRNA design but target gene promoter regions rather than the coding sequence. The promoter-targeting sgRNA, then, recruits a modified dCas9 protein which either activates or represses gene expression—depending on the dCas9 modification. Cellecta’s CRISPRa and CRISPRi Human Genome-Wide sgRNA Libraries have been designed and optimized for pooled activator and repressor genetic screens.

Pooled screens can be run with both the CRISPR-activation (CRISPRa) and CRISPR-interference (CRISPRi) systems. Cellecta’s CRISPRa and CRISPRi human genome-wide sgRNA libraries have been designed with the following features to facilitate these screens:

  • Single-Module libraries–each expressing ~106,000 sgRNA
  • Each library targets almost 19,000 human protein-coding genes
  • Each gene is targeted by 5 sgRNAs designed against the promoter region (designs based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554)
  • 3,700 non-targeting sgRNA controls
  • Convenient quantities provided: 200 ug plasmid; 2 x 10^8 TU and 1 x 10^9 TU packaged viral libraries
  • Dual-guide CRISPRa and CRISPRi libraries are also available

 

Human Genome-Wide CRISPRa sgRNA Library

Selective activation of targeted genes when used in cells expressing dCas9-VPH (or other dCas9-activator variations compatible with spCas9 sgRNA designs)

Human Genome-Wide CRISPRi sgRNA Library

Selective expression inhibition of targeted genes when used in cells expressing dCas9-KRAB (or other deactivated Cas9 repressors compatible with spCas9 sgRNA designs)

Mouse Genome-Wide CRISPRa and CRISPRi libraries are also available.

Ordering information

NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email orders@cellecta.com using your institutional email account, and ask for a quote with the discounted pricing.

 

Quantity provided in each library: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 TU packaged

Human Genome-Wide CRISPRa sgRNA Library (single-guide)

Catalog#NameCategoryPriceBuy
KAHGW-106-PHuman Genome-Wide CRISPRa sgRNA Library (plasmid)$10,000.00
KAHGW-106-V8Human Genome-Wide CRISPRa sgRNA Library (virus)$8,500.00
KAHGW-106-V9Human Genome-Wide CRISPRa sgRNA Library (virus)$18,000.00

Human Genome-Wide CRISPRa Dual-sgRNA Library (dual-guide)

Catalog#NameCategoryPriceBuy
KADHGW-105K-PHuman Genome-Wide CRISPRa Dual-sgRNA Library (plasmid)$12,000.00
KADHGW-105K-V8Human Genome-Wide CRISPRa Dual-sgRNA Library (virus)$9,500.00
KADHGW-105K-V9Human Genome-Wide CRISPRa Dual-sgRNA Library (virus)$20,000.00

Human Genome-Wide CRISPRi sgRNA Library (single-guide)

Catalog#NameCategoryPriceBuy
KIHGW-106-PHuman Genome-Wide CRISPRi sgRNA Library (plasmid)$10,000.00
KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)$8,500.00
KIHGW-106-V9Human Genome-Wide CRISPRi sgRNA Library (virus)$18,000.00

Human Genome-Wide CRISPRi Dual-sgRNA Library (dual-guide)

Catalog#NameCategoryPriceBuy
KIDHGW-105K-PHuman Genome-Wide CRISPRi Dual-sgRNA Library (plasmid)$12,000.00
KIDHGW-105K-V8Human Genome-Wide CRISPRi Dual-sgRNA Library (virus)$9,500.00
KIDHGW-105K-V9Human Genome-Wide CRISPRi Dual-sgRNA Library (virus)$20,000.00

If you are interested in a custom library, Cellecta can help. Visit our Order page for specific instructions.

User Manual

Gene Lists

sgRNA Sequences

  • Sequences are provided upon shipment of library.

Vector Information

Single sgRNA Library VectorNGS CassetteSequence
(FASTA|GenBank|SnapGene*)
Map
pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-PuroHTS6C.txt | .gb | .dnaMap

Dual-sgRNA Library VectorNGS CassetteSequence
(FASTA|GenBank|SnapGene*)
Map
pRSL10-mU6-sgRNA1-hU6-sgRNA2-UbiC-TagRFP-2A-PuropRSL10.txt | .gb | .dnaMap

Product Certificates

Lot NumberCatalog NumberDescriptionQuantityProduct Certificate
181128001KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)2 x 108 TUPDF
180124001KIHGW-106-PHuman Genome-Wide CRISPRi sgRNA Library (plasmid)200 µgPDF
18011730KAHGW-106-V8Human Genome-Wide CRISPRa sgRNA Library (virus)2 x 108 TUPDF
18011702KIHGW-106-V8Human Genome-Wide CRISPRi sgRNA Library (virus)2 x 108 TUPDF
17122701KAHGW-106-PHuman Genome-Wide CRISPRa sgRNA Library (plasmid)200 µgPDF

 

Safety Data Sheets (SDS)

Next-Gen Sequencing and Analysis

Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies sgRNA
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerates sgRNA counts from raw sequencing data

Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.

dCas9 Expression Plasmids

More information >

Catalog#NameCategoryPriceBuy
SVKRABC9B-PSCRISPRi dCas9-KRAB pRDKCCB-CMV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9E2B-PSCRISPRi dCas9-KRAB pRDKCE2B-EFS-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9H-PSCRISPRi dCas9-KRAB pRDKCCH-CMV-dCas9-KRAB-2A-Hygro (plasmid)$300.00
SVKRABC9RB-PSCRISPRi dCas9-KRAB pRDKCRB-RSV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9SFB-PSCRISPRi dCas9-KRAB pRDKCSFB-SFFV-dCas9-KRAB-2A-Blast (plasmid)$300.00
SVKRABC9B-VSCRISPRi dCas9-KRAB pRDKCCB-CMV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9E2B-VSCRISPRi dCas9-KRAB pRDKCE2B-EFS-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9H-VSCRISPRi dCas9-KRAB pRDKCCH-CMV-dCas9-KRAB-2A-Hygro (virus)$500.00
SVKRABC9RB-VSCRISPRi dCas9-KRAB pRDKCRB-RSV-dCas9-KRAB-2A-Blast (virus)$500.00
SVKRABC9SFB-VSCRISPRi dCas9-KRAB pRDKCSFB-SFFV-dCas9-KRAB-2A-Blast (virus)$500.00
Catalog#NameCategoryPriceBuy
SVVPHC9B-PSCRISPRa dCas9-VPH pRDVCCB-CMV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9E2B-PSCRISPRa dCas9-VPH pRDVCE2B-EFS-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9RB-PSCRISPRa dCas9-VPH pRDVCRB-RSV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9SFB-PSCRISPRa dCas9-VPH pRDVCSFB-SFFV-dCas9-VPH-2A-Blast (plasmid)$300.00
SVVPHC9B-VSCRISPRa dCas9-VPH pRDVCCB-CMV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9E2B-VSCRISPRa dCas9-VPH pRDVCE2B-EFS-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9RB-VSCRISPRa dCas9-VPH pRDVCRB-RSV-dCas9-VPH-2A-Blast (virus)$500.00
SVVPHC9SFB-VSCRISPRa dCas9-VPH pRDVCSFB-SFFV-dCas9-VPH-2A-Blast (virus)$500.00

We can also provide other configurations of dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.

Catalog#NameCategoryPriceBuy
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix$340.00
LNGS-120NGS Prep Kit for sgRNA Libraries in pRSG16/17 (KOHGW)$875.00
LNGS-120-SPSupplementary Primer Set for LNGS-120 (12 Additional Index Primers)$475.00
LFVC1LentiFuge™ Viral Concentration Reagent$325.00
LTDR1LentiTrans™ Transduction Reagent (Polybrene for Transduction)$175.00
LTSET-GLentiPrep™ Lentiviral Reagent Set with GFP Control for Packaging and Transduction$750.00
LTSET-RLentiPrep™ Lentiviral Reagent Set with RFP Control for Packaging and Transduction$750.00
CRATESTCRISPRaTest™ Functional dCas9-Activator Assay Kit$850.00
CRITESTCRISPRiTest™ Functional dCas9-Repressor Assay Kit$850.00
CRITP-SETCRISPRiTest™ dCas9-Repressor Assay / 4x dCas9-KRAB (SVKRABC9P-VS, SVKRABC9E2B-VS, SVKRABC9RP-VS, SVKRABC9SFB-VS)$1,850.00
CRATP-SETCRISPRaTest™ dCas9-Repressor Assay / 4x dCas9-KRAB (SVKRABC9P-VS, SVKRABC9E2B-VS, SVKRABC9RP-VS, SVKRABC9SFB-VS)$1,850.00
PROMTESTPromoterTest™ Assay Kit (8 promoter constructs)$895.00