CRISPRa and CRISPRi Mouse Genome-Wide Libraries
The original CRISPR-Cas9 complex enables highly targeted knockout of essentially any gene but variations of the CRISPR system use engineered versions of the deactivated Cas9 (dCas9) protein to either interfere (i.e., repress) or activate gene expression without modifying genomic DNA. These gene-regulating variations of CRISPR use the same basic sgRNA design, but target gene promoter regions rather than the coding region. The promoter targeting sgRNA, then, recruits a modified dCas9 protein which either activates or represses gene expression—depending on the dCas9 modification. Cellecta’s CRISPRa and CRISPRi Mouse Genome-Wide sgRNA Libraries have been designed and optimized for pooled activator and repressor genetic screens.
Pooled screens can be run with both the CRISPR-activation (CRISPRa) and CRISPR-interference (CRISPRi) systems. Cellecta’s CRISPRa and CRISPRi mouse genome-wide sgRNA libraries have been designed with the following features to facilitate these screens:
- Single-Module libraries–each expressing ~109,000 sgRNA
- Each library targets almost 20,000 mouse protein-coding genes
- Each gene is targeted by 5 sgRNAs designed against the promoter region (designs based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554)
- 3,700 non-targeting sgRNA controls
- Convenient quantities available: 200 ug plasmid; 2 x 10^8 TU and 1 x 10^9 TU packaged
Mouse Genome-Wide CRISPRa sgRNA Library
Selective activation of targeted genes when used in cells expressing dCas9-VPH (or other dCas9-activator variations compatible with spCas9 sgRNA designs)
Mouse Genome-Wide CRISPRi sgRNA Library
selective expression inhibition of targeted genes when used in cells expressing dCas9-KRAB (or other deactivated Cas9 repressors compatible with spCas9 sgRNA designs)
Human genome-wide CRISPRa and CRISPRi libraries are also available.
NOTE: Cellecta offers academic discounts on many of our pre-packaged libraries to qualifying academic institutions. Please email firstname.lastname@example.org using your institutional email account, to ask for a quote with the discounted pricing.
Quantity provided in each library: 200 ug plasmid; 2 x 10^8 TU or 1 x 10^9 TU packaged
If you are interested in a custom library, Cellecta can help. Visit our Order page for specific instructions.
- CRISPR Pooled Lentiviral sgRNA Libraries (Online Manual or PDF)
- Sequences are provided upon shipment of library.
|Single sgRNA Library Vector||NGS Cassette||Sequence|
|pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-Puro||HTS6C||.txt | .gb | .dna||Map|
|Lot Number||Catalog Number||Description||Quantity||Product Certificate|
|180124003||KAMGW-109-V9||Mouse Genome-Wide CRISPRa sgRNA Library (virus)||1 x 109 TU|
|180124002||KIMGW-109-P||Mouse Genome-Wide CRISPRi sgRNA Library (plasmid)||200 µg|
|18010501||KAMGW-109-P||Mouse Genome-Wide CRISPRa sgRNA Library (plasmid)||200 µg|
Safety Data Sheets (SDS)
Next-Gen Sequencing and Analysis
Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.
You provide Cellecta with frozen cell pellets or tissue after screening…
…and Cellecta does the rest:
- Extracts genomic DNA
- Amplifies sgRNA
- Performs NGS on the Illumina NextSeq or HiSeq
- Enumerates sgRNA counts from raw sequencing data
Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.
dCas9 Expression Plasmids
We can also provide other configurations of dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.
|Catalog #||Item Description||Price|
|CPCP-K2A||Ready-to-Use Lentiviral Packaging Plasmid Mix, 250 μg|
(for (25) 10-cm plates)
|LFVC1||LentiFuge™ Viral Concentration Reagent, 1 ml|
(for 1 liter supernatant)
|CRATEST||CRISPRaTest™ Functional dCas9-Activator Assay Kit||$850|
|CRITEST||CRISPRiTest™ Functional dCas9-Repressor Assay Kit||$850|
|LNGS-120||NGS Prep Kit for KOHGW CRISPR sgRNA libraries||$875|