Three-Module Human Genome-Wide CRISPR Library
Cellecta’s original Genome-Wide CRISPR Knockout Pooled sgRNA Library was designed as three modules, each targeting approximately a third of the genes with eight sgRNAs per gene, which is more than most other libraries use. In addition to filtering to ensure guides are unique for each gene target to minimize false positives, the sgRNAs in Cellecta’s Three-Module Human Genome-Wide CRISPR Library incorporate Cellecta’s HEAT design sgRNA structure which has been shown to generate more robust screening results than the standard sgRNA design.
Deep Genome-Wide Coverage of All Human Genes
- 3 Modules, each covering approximately 6,300 genes
- Each gene is targeted by up to 8 sgRNA for a total of approximately 50,000 sgRNA per module
- Targets all human protein-coding genes—over 19,000 gene targets
- Available in convenient quantities: 200 ug plasmid, 2 x 10^8 TU or 1 x 10^9 TU packaged
Three separate modules, each with approximately fifty thousand constructs provide a practical approach to screens with more guides per target. The modular format is similar to Cellecta’s Human Genome-Wide shRNA Library, and each of the CRISPR and RNAi Library modules target the same sets of genes so that investigators can more easily make use of both techniques for genome-wide loss of function screens.
Depending on the needs of the experiment, each of the three CRISPR Library modules can be used separately or combined for a single genome-wide knockout screen. For example, screens with a strong selection and few surviving cells may be easily run using a single large pool with all three modules combined. However, with “dropout viability” screens that require maintenance of hundreds of millions of cells for weeks at a time, individual screens with each module run separately, may be more practical.
The selection of genes targeted with each module also allows researchers primarily interested in therapeutic applications to focus on the most relevant and annotated set of disease related targets by screen Modules 1 and/or 2. The complete list of genes targeted by each modules is available below.
- CRISPR Human Genome Knockout Module 1 targets the most annotated genes, including members of major signal transduction pathways, genes from the Cancer Genome Atlas (TCGA), and approved drug targets.
- Module 2 targets additional disease-associated genes and drug targets
- Module 3 targets cell surface markers, extracellular matrix genes, and DNA binding genes
NOTE: Cellecta offers academic discounts on many of our plasmid and pre-packaged libraries to qualifying academic institutions. Please email us at email@example.com using your institutional email account, and ask for a quote with the discounted pricing.
We can also prepare custom libraries according to your specifications. Email firstname.lastname@example.org for details or to obtain a quote.
Quantity provided in each library: 200 ug plasmid
Packaged Viral Library
Quantity provided in each library: 2 x 10^8 TU or 1 x 10^9 TU packaged
- CRISPR Pooled Lentiviral sgRNA Libraries (Online Manual or PDF)
The target gene lists for each module of the Three-Module Human Genome-Wide CRISPR Library can be downloaded here:
- CRISPR Human Genome KO Library Module 1
- CRISPR Human Genome KO Library Module 2
- CRISPR Human Genome KO Library Module 3
|CRISPR Library Vector||NGS Cassette||Sequence|
|pRSG16-U6-sg-HTS6C-UbiC-TagRFP-2A-Puro||HTS6C||.txt | .gb | .dna||Map|
|Lot Number||Catalog Number||Description||Quantity||Product Certificate|
|180124006||KOHGW-M3-V9||CRISPR Human Genome Knockout Library, Module 3 (virus)||1 x 109 TU|
|180124005||KOHGW-M2-V9||CRISPR Human Genome Knockout Library, Module 2 (virus)||1 x 109 TU|
|180124004||KOHGW-M1-V9||CRISPR Human Genome Knockout Library, Module 1 (virus)||1 x 109 TU|
|15082703||KOHGW-M3-V8||CRISPR Human Genome Knockout Library, Module 3 (virus)||2 x 108 TU|
|15082702||KOHGW-M2-V8||CRISPR Human Genome Knockout Library, Module 2 (virus)||2 x 108 TU|
|15082701||KOHGW-M1-V8||CRISPR Human Genome Knockout Library, Module 1 (virus)||2 x 108 TU|
|15082406||KOHGW-M3-P||CRISPR Human Genome Knockout Library, Module 3 (plasmid)||200 µg|
|15082404||KOHGW-M2-P||CRISPR Human Genome Knockout Library, Module 2 (plasmid)||200 µg|
|15082402||KOHGW-M1-P||CRISPR Human Genome Knockout Library, Module 1 (plasmid)||200 µg|
Safety Data Sheets (SDS)
* Download the SnapGene® Viewer to open .dna format files.
Next-Gen Sequencing and Analysis
Cellecta CRISPR sgRNA Libraries are provided with a complete protocol and all sequencing information to enable researchers to perform experiments and extract the data. However, we also provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells or tissue for each time point or treatment condition (one sample), and we’ll extract DNA, amplify the sgRNA sequences, perform NGS, and assemble the data with some basic analysis.
You provide Cellecta with frozen cell pellets or tissue after screening…
…and Cellecta does the rest:
- Extracts genomic DNA
- Amplifies sgRNA
- Performs NGS on the Illumina NextSeq or HiSeq
- Enumerates sgRNA counts from raw sequencing data
Please see the Next-Gen Sequencing and Analysis web page for additional information and instructions on how to order.
Cas9 Expression Plasmids
We can also provide other configurations of Cas9 or dCas9 expression plasmids on a custom basis. Please inquire if you require something different than the items described above.