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Next-Generation Sequencing (NGS) Analysis Kits

For Pooled CRISPR, RNAi and Barcode Libraries

Screens using complex pooled CRISPR and RNAi libraries rely on next-generation sequencing (NGS) read counts to determine the relative representation of each library element in the genomic DNA of each screened cell or tissue sample. Preparation of these short, specific library elements–shRNA, sgRNA or barcodes–for sequencing requires targeted PCR amplification with specially designed primer, followed by sequencing with custom primers.

More than 15 custom primers are used in the multiplex analysis of the samples. These are specific to each library/vector combination.

Simply select the NGS Analysis kit corresponding to your library and email us at orders@cellecta.com

 

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Catalog # Item Description Amount Price
LNGS-101 NGS Prep Kit for DECIPHER (shRNA barcoded libraries in pRSI12) 1 kit – 24 samples $875
LNGS-102 NGS Prep Kit for HGW shRNA (barcoded libraries in pRSI16/pRSI17) 1 kit – 24 samples $875
LNGS-120 NGS Prep Kit for KOHGW CRISPR (sgRNA libraries in pRSG16/pRSG17) 1 kit – 24 samples $875
LNGS-200 NGS Prep Kit for CellTracker Barcode Libraries (pRSI16/pRSI17) 1 kit – 24 samples $875
LNGS-900 NGS Prep Kit for LentiGuide sgRNA Libraries (e.g. GeCKO, Brunello, Brie) 1 kit – 24 samples $875
LNGS-905 NGS Prep Kit for LentiCRISPRv2 sgRNA Libraries (e.g. GeCKO single vector, Brunello Kinome) 1 kit – 24 samples $875

Resources

Product flyer

     

  • Next-Generation Sequencing (NGS) Analysis Kits for Pooled Libraries (PDF)