Next-Generation Sequencing (NGS) Analysis Kits
For Pooled CRISPR, RNAi and Barcode Libraries
Screens using complex pooled CRISPR and RNAi libraries rely on next-generation sequencing (NGS) read counts to determine the relative representation of each library element in the genomic DNA of each screened cell or tissue sample. Preparation of these short, specific library elements–shRNA, sgRNA or barcodes–for sequencing requires targeted PCR amplification with specially designed primer, followed by sequencing with custom primers.
More than 15 custom primers are used in the multiplex analysis of the samples. These are specific to each library/vector combination.
Simply select the NGS Analysis kit corresponding to your library and email us at email@example.com
|Catalog #||Item Description||Amount||Price|
|LNGS-101||NGS Prep Kit for DECIPHER (shRNA barcoded libraries in pRSI12)||1 kit – 24 samples||$875|
|LNGS-102||NGS Prep Kit for HGW shRNA (barcoded libraries in pRSI16/pRSI17)||1 kit – 24 samples||$875|
|LNGS-120||NGS Prep Kit for KOHGW CRISPR (sgRNA libraries in pRSG16/pRSG17)||1 kit – 24 samples||$875|
|LNGS-200||NGS Prep Kit for CellTracker Barcode Libraries (pRSI16/pRSI17)||1 kit – 24 samples||$875|
|LNGS-900||NGS Prep Kit for LentiGuide sgRNA Libraries (e.g. GeCKO, Brunello, Brie)||1 kit – 24 samples||$875|
|LNGS-905||NGS Prep Kit for LentiCRISPRv2 sgRNA Libraries (e.g. GeCKO single vector, Brunello Kinome)||1 kit – 24 samples||$875|
- Next-Generation Sequencing (NGS) Analysis Kits for Pooled Libraries (PDF)