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Next-Generation Sequencing (NGS) Analysis Kits

For Pooled CRISPR, RNAi and Barcode Library Screening Samples

Screens using complex pooled CRISPR and RNAi libraries rely on NGS read counts to determine the relative representation of each library element in the genomic DNA of each screened cell or tissue sample. Preparation of these short, specific library elements–shRNA, sgRNA or barcodes–for sequencing requires targeted PCR amplification with specially designed primer, followed by sequencing with custom primers.

More than 15 custom primers are used in the multiplex NGS analysis of the samples. These are specific to each library/vector combination.

Each kit contains primers for 6-48 samples (48 preps of 50 ug each, 12 multiplex).

Simply select the NGS Analysis kit corresponding to your library and order below.

For any additional questions, contact sales@cellecta.com.

Catalog#NameCategoryPriceBuy
LNGS-200NGS Prep Kit for Barcode Libraries in pRSI16/17 (CellTracker 50M)$875.00
875
LNGS-905NGS Prep Kit for sgRNA Libraries in LentiCRISPRv2 (GeCKO, Brunello Kinome)$875.00
875
LNGS-900NGS Prep Kit for sgRNA Libraries in LentiGuide (GeCKO, Brunello)$875.00
875
LNGS-120NGS Prep Kit for sgRNA Libraries in pRSG16/17 (KOHGW)$875.00
875
LNGS-130NGS Prep Kit for sgRNA Libraries in pRSGT16/17$875.00
875
LNGS-101NGS Prep Kit for shRNA Libraries in pRSI12 (DECIPHER)$875.00
875
LNGS-102NGS Prep Kit for shRNA Libraries in pRSI16cb/17cb (HGW)$875.00
875

Resources

Product Flyer

  • Next-Generation Sequencing (NGS) Analysis Kits for Pooled Libraries (PDF)

User Manual