Next-Generation Sequencing (NGS) Analysis Kits
For Pooled CRISPR, RNAi, and Barcode Library Screening Samples
Screens using complex pooled CRISPR, RNAi, and barcode libraries rely on NGS read counts to determine the relative representation of each library element in the genomic DNA of each screened cell or tissue sample. Preparation of these short, specific library elements–sgRNA, shRNA, or barcodes–for sequencing requires targeted PCR amplification with specially designed primers, followed by sequencing with custom NGS primers.
More than 15 custom primers are used in the multiplex NGS analysis of the samples. These are specific to each library/vector combination. Each kit contains primers for 6-48 samples (48 preps of 50 ug each, 12 multiplex). Supplementary indexing primers are also available, which increase multiplexing to capacity to 24 samples.
Simply select the NGS Analysis kit corresponding to your library and order below.
For any additional questions, contact firstname.lastname@example.org.
Supplementary indexing primers are also available for each of the LNGS kits. When used with the corresponding LNGS-xxx kits, these enable multiplexing of an additional 12 samples, for a total of 24 samples.