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Cloning and Expression Vector Information

Cellecta offers a comprehensive collection of cloning and expression vectors for individual constructs to pooled library generation. Find all vector information here, including cloning/Next-Gen Sequencing (NGS) cassette and primer design maps, sequences, GenBank/Vector NTI® and SnapGene® files, and vector maps. Please note that this list is not comprehensive. For a complete listing of a specific vector type, please contact us.

Cellecta’s vector collection includes the following types of expression vectors and accessory plasmids. Linearized shRNA/sgRNA vectors or expression vectors with multiple cloning sites are also available, so you may clone your own inserts.

Cellecta can also customize vectors. Please inquire for more information. Choose from a variety of vector elements, and we’ll build your vector:
 

  • Constitutive or inducible versions of H1 or U6 shRNA/sgRNA promoters
  • TagRFP and TagGFP2 fluorescent markers, PuroR, BleoR, NeoR, BlastR, and HygroR antibiotic-resistance markers, and Thy1.1 cell surface marker, or others of your choice
  • CMV, EF1, UbiC, PGK, or others of your choice

shRNA Knockdown Vectors

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Constitutive shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSI12-U6-(sh)-UbiC-TagRFP-2A-PuroDVSHC-LHTS4.txt|.gbk|.dnaMap
pRSI12N-U6-(sh)-UbiC-TagRFP-2A-NeoSVSHU612N-LHTS4.txt|.gbk|.dnaMap
pRSI16-U6-(sh)-UbiC-TagRFP-2A-PuroSVSHU616-LHTS6.txt|.gbk|.dnaMap
pRSI16Thy-U6-(sh)-UbiC-TagRFP-2A-Thy1.1SVSHU616Thy-LHTS6.txt|.gb|.dnaMap
pRSI16cb-U6-(sh)-13kCB18-UbiC-TagRFP-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI17-U6-(sh)-UbiC-TagGFP2-2A-PuroSVSHU617-LHTS6.txt|.gbk|.dnaMap
pRSI17H-U6-(sh)-UbiC-TagGFP2-2A-HygroSVSHU617H-LHTS6.txt|.gb|.dnaMap
pRSI17cb-U6-(sh)-13kCB18-UbiC-TagGFP2-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI20-U6-(sh)-CMV-TagRFP-2A-PuroSVSHU620-LHTS6.txt|.gbk|.dnaMap
pRSI21-U6-(sh)-CMV-TagGFP2-2A-PuroSVSHU621-LHTS6.txt|.gb|.dnaMap
pRSIUP-U6-(sh)-UbiC-PuroSVSHU6UP-LHTS6.txt|.gbk|.dnaMap
pRSIUThy-U6-(sh)-UbiC-Thy1.1SVSHU6UThy-LHTS6.txt|.gb|.dnaMap
pRSI7-H1-(sh)-UbiC-TagRFP-2A-PuroSVSHH116-LPlease inquire.txt|.gbk|.dnaMap
pMSCVURP-U6-(sh)-UbiC-TagRFP-2A-Puro (retroviral)SVSHMVU6URP-LHTS6M (not for linear vector)Library: .txt|.gb|.dna
Linear Vector: .txt|.gb|.dna
Library: Map
Linear Vector: Map


Inducible shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSIT12-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T12-LHTS4.txt|.gb|.dnaMap
pRSIT16-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T16-LHTS6.txt|.gbk|.dnaMap
pRSIT16cb-U6Tet-(sh)-13kCB18-CMV-TetRep-2A-TagRFP-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gb|.dnaMap
pRSIT17-U6Tet-(sh)-CMV-TetRep-2A-TagGFP2-2A-PuroSVSHU6T17-LHTS6.txt|.gb|.dnaMap
pRSITEP-U6Tet-(sh)-EF1-TetRep-2A-PuroSVSHU6TEP-Lno HTS.txt|.gb|.dnaMap
pRSITER-U6Tet-(sh)-EF1-TetRep-2A-TagRFPSVSHU6TER-Lno HTS.txt|.gb|.dnaMap
pRSITUR-U6Tet-(sh)-UbiC-TetRep-2A-TagRFPSVSHU6TUR-LHTS3.txt|.gb|.dnaMap

CRISPR, CRISPRi, and CRISPRa sgRNA Expression Vectors

Single-Vector CRISPR sgRNA/Cas9 Knockout System

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGCCP-U6-(sg)-CMV-Cas9-2A-Puro (was pRSGC1)SVCRU6CCP-LHTS6C.txt|.gb|.dnaMap
pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hygro (was pRSGC3)SVCRU6CCH-LHTS6C.txt|.gb|.dnaMap
pRSGCCB-U6-(sg)-CMV-Cas9-2A-Blast(was pRSGC5)SVCRU6CCB-LHTS6C.txt|.gb|.dnaMap
pRSGCCN-U6-(sg)-CMV-Cas9-2A-Neo(was pRSGC6)SVCRU6CCN-LHTS6C.txt|.gbk|.dnaMap
pRSGCCG-U6-(sg)-CMV-Cas9-2A-TagGFP2 (was pRSGC4)SVCRU6CCG-LHTS6C.txt|.gb|.dnaMap
pRSGCCR-U6-(sg)-CMV-Cas9-2A-TagRFP(was pRSGC7)SVCRU6CCR-LHTS6C.txt|.gb|.dnaMap
pRSGCEH-U6-(sg)-EF1-Cas9-2A-HygroSVCRU6CEH-LHTS6C.txt|.gb|.dnaMap
pRSGCUP-U6-(sg)-UbiC-Cas9-2A-Puro (was pRSGC2)SVCRU6UCP-LHTS6C.txt|.gbk|.dnaMap
pRSGCUR-U6-(sg)-UbiC-Cas9-2A-TagRFPSVCRU6UCR-LHTS6C.txt|.gb|.dnaMap

Two Vector System (sgRNA-Only Expression Vectors)

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSG16-U6-(sg)-UbiC-TagRFP-2A-PuroSVCRU616-LHTS6C.txt|.gbk|.dnaMap
pRSG16Thy-U6-(sg)-UbiC-TagRFP-2A-Thy1.1SVCRU616Thy-LHTS6C.txt|.gb|.dnaMap
pRSG17-U6-(sg)-UbiC-TagGFP2-2A-PuroSVCRU617-LHTS6C.txt|.gbk|.dnaMap
pRSG20-U6-(sg)-CMV-TagRFP-2A-PuroSVCRU620-LHTS6C.txt|.gb|.dnaMap
pRSG21-U6-(sg)-CMV-TagGFP2-2A-PuroSVCRU621-LHTS6C.txt|.gb|.dnaMap
pRSGEP-U6-(sg)-EF1-PuroSVCRU6EP-LHTS6C.txt|.gb|.dnaMap
pRSGUP-U6-(sg)-UbiC-PuroSVCRU6UP-LHTS6C.txt|.gb|.dnaMap
pRSGUThy-U6-(sg)-UbiC-Thy1.1SVCRU6UThy-LHTS6C.txt|.gb|.dnaMap

Inducible sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-TagRFP-2A-PuroSVCRU6T16-LPlease Inquire.txt|.gb|.dnaMap
pRSGT17-U6Tet-(sg)-CMV-TetRep-2A-TagGFP2-2A-PuroSVCRU6T17-LPlease Inquire.txt|.gb|.dnaMap
pRSGTEP-U6Tet-(sg)-EF1-TetRep-2A-PuroSVCRU6TEP-Lno HTS.txt|.gb|.dnaMap
pRSGTER-U6Tet-(sg)-EF1-TetRep-2A-TagRFPSVCRU6TER-Lno HTS.txt|.gb|.dnaMap
pRSGTEBleo-U6Tet-(sg)-EF1-TetRep-2A-BleoSVCRU6TEBleo-Lno HTS.txt|.gb|.dnaMap

Dual-sgRNA Expression Vectors (Two Vector System)

Please contact us for more details.

Dual sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRDSG16-U6M3-sg1-U6-sg2-UbiC-TagRFP-2A-PuroPlease InquirePlease Inquire.txt|.gbk|.dnaMap

Cas9 and dCas9-Expressing Plasmids

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Cas9-Only VectorsCatalog #Sequence**Map
pRCCH-CMV-Cas9-2A-HygroSVC9-PS.txt|.gb|.dnaMap
pRCCB-CMV-Cas9-2A-BlastSVC9B-PS.txt|.gb|.dnaMap
pRDKCCB-CMV-dCas9-KRAB-2A-Blast (CRISPRi)SVKRABC9B-PS.txt|.gbk|.dnaMap
pRDKCCH-CMV-dCas9-KRAB-2A-Hygro (CRISPRi)SVKRABC9H-PS.txt|.gb|.dnaMap
pRDVCCB-CMV-dCas9-VPH-2A-Blast (CRISPRa)SVVPHC9B-PS.txt|.gbk|.dnaMap

cDNA Overexpression Vectors

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cDNA Cloning VectorsCatalog #ORF PromoterMCSSequence**Map
pRCDEM-EF1-MCSPRCDEMEF1MCS.txt|.gb|.dnaMap
pRCDEMB-EF1-MCS-2A-BleoPRCDEMBEF1MCS.txt|.gb|.dnaMap
pRCDEMP-EF1-MCS-2A-PuroPRCDEMPEF1MCS.txt|.gb|.dnaMap
pRCDCBEM-CMV-Bleo-EF1-MCSPRCDCBEMEF1MCS.txt|.gb|.dnaMap
pRCDCM-CMV-MCSPRCDCMCMVMCS.txt|.gb|.dnaMap
pRCDCMEB-CMV-MCS-EF1-BleoPRCDCMEBCMVMCS.txt|.gb|.dnaMap
pRCDCMEG-CMV-MCS-EF1-TagGFP2PRCDCMEGCMVMCS.txt|.gb|.dnaMap
pRCDCMEN-CMV-MCS-EF1-NeoPRCDCMENCMVMCS.txt|.gb|.dnaMap
pRCDCMEP-CMV-MCS-EF1-PuroPRCDCMEPCMVMCS.txt|.gb|.dnaMap
pRCDCMER-CMV-MCS-EF1-TagRFPPRCDCMERCMVMCS.txt|.gb|.dnaMap
pRCDCMERP-CMV-MCS-EF1-TagRFP-2A-PuroPRCDCMERPCMVMCS.txt|.gb|.dnaMap
pRCDCMP-CMV-MCS-2A-PuroPRCDCMPCMVMCS.txt|.gb|.dnaMap
pRCDCMRB-CMV-MCS-2A-TagRFP-2A-PuroPRCDCMRBCMVMCS.txt|.gb|.dnaMap

* Only vectors with NGS cassettes can be used for library construction.

** Sequences are available in FASTA (.txt), GenBank (.gb/.gbk), and SnapGene® (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us.


For information on the safety of our lentiviruses, please download our Lentivirus SDS.