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Cloning and Expression Vector Information

Cellecta offers a comprehensive collection of shRNA, CRISPR, CRISPRi, CRISPRa, and cDNA cloning and expression vectors for individual construct to pooled library generation. Find all vector information here, including cloning/Next-Gen Sequencing (NGS) cassette and primer design maps, sequences, GenBank/Vector NTI® and SnapGene® files, and vector maps.

 

shRNA Knockdown Vectors

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Constitutive shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSI12-U6-(sh)-UbiC-TagRFP-2A-PuroDVSHC-LHTS4.txt|.gbk|.dnaMap
pRSI12N-U6-(sh)-UbiC-TagRFP-2A-NeoSVSHU612N-LHTS4.txt|.gbk|.dnaMap
pRSI16-U6-(sh)-UbiC-TagRFP-2A-PuroSVSHU616-LHTS6.txt|.gbk|.dnaMap
pRSI16Thy-U6-(sh)-UbiC-TagRFP-2A-Thy1.1SVSHU616Thy-LHTS6.txt|.gb|.dnaMap
pRSI16cb-U6-(sh)-13kCB18-UbiC-TagRFP-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI17-U6-(sh)-UbiC-TagGFP2-2A-PuroSVSHU617-LHTS6.txt|.gbk|.dnaMap
pRSI17H-U6-(sh)-UbiC-TagGFP2-2A-HygroSVSHU617H-LHTS6.txt|.gb|.dnaMap
pRSI17cb-U6-(sh)-13kCB18-UbiC-TagGFP2-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI20-U6-(sh)-CMV-TagRFP-2A-PuroSVSHU620-LHTS6.txt|.gbk|.dnaMap
pRSI21-U6-(sh)-CMV-TagGFP2-2A-PuroSVSHU621-LHTS6.txt|.gbk|.dnaMap
pRSIUP-U6-(sh)-UbiC-PuroSVSHU6UP-LHTS6.txt|.gbk|.dnaMap
pRSIUThy-U6-(sh)-UbiC-Thy1.1SVSHU6UThy-LHTS6.txt|.gb|.dnaMap
pRSI7-H1-(sh)-UbiC-TagRFP-2A-PuroSVSHH116-LPlease inquire.txt|.gbk|.dnaMap
pMSCVURP-U6-(sh)-UbiC-TagRFP-2A-Puro (retroviral)SVSHMVU6URP-LHTS6M (not for linear vector)Library: .txt|.gb|.dna
Linear Vector: .txt|.gb|.dna
Library: Map
Linear Vector: Map
Inducible shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSIT12-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T12-LHTS4.txt|.gb|.dnaMap
pRSIT16-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T16-LHTS6.txt|.gbk|.dnaMap
pRSIT17-U6Tet-(sh)-CMV-TetRep-2A-TagGFP2-2A-PuroSVSHU6T17-LHTS6.txt|.gb|.dnaMap
pRSITEP-U6Tet-(sh)-EF1-TetRep-2A-PuroSVSHU6TEP-Lno HTS.txt|.gbk|.dnaMap
pRSITUR-U6Tet-(sh)-UbiC-TetRep-2A-TagRFPSVSHU6TUR-LHTS3.txt|.gbk|.dnaMap

 

CRISPR, CRISPRi, and CRISPRa sgRNA Expression Vectors

Single Vector CRISPR sgRNA-Cas9 Knockout System

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGCCP-U6-(sg)-CMV-Cas9-2A-Puro (was pRSGC1)SVCRU6CCP-LHTS6C.txt|.gb|.dnaMap
pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hygro (was pRSGC3)SVCRU6CCH-LHTS6C.txt|.gbk|.dnaMap
pRSGCCB-U6-(sg)-CMV-Cas9-2A-Blast (was pRSGC5)SVCRU6CCB-LHTS6C.txt|.gb|.dnaMap
pRSGCCN-U6-(sg)-CMV-Cas9-2A-Neo (was pRSGC6)SVCRU6CCN-LHTS6C.txt|.gbk|.dnaMap
pRSGCCG-U6-(sg)-CMV-Cas9-2A-TagGFP2 (was pRSGC4)SVCRU6CCG-LHTS6C.txt|.gb|.dnaMap
pRSGCCR-U6-(sg)-CMV-Cas9-2A-TagRFP (was pRSGC7)SVCRU6CCR-LHTS6C.txt|.gb|.dnaMap
pRSGCUP-U6-(sg)-UbiC-Cas9-2A-Puro (was pRSGC2)SVCRU6UCP-LHTS6C.txt|.gbk|.dnaMap

Two Vector System (sgRNA-Only Expression Vectors)

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSG16-U6-(sg)-UbiC-TagRFP-2A-PuroSVCRU616-LHTS6C.txt|.gbk|.dnaMap
pRSG16Thy-U6-(sg)-UbiC-TagRFP-2A-Thy1.1SVCRU616Thy-LHTS6C.txt|.gb|.dnaMap
pRSG17-U6-(sg)-UbiC-TagGFP2-2A-PuroSVCRU617-LHTS6C.txt|.gbk|.dnaMap
pRSG20-U6-(sg)-CMV-TagRFP-2A-PuroSVCRU620-LHTS6C.txt|.gb|.dnaMap
pRSG21-U6-(sg)-CMV-TagGFP2-2A-PuroSVCRU621-LHTS6C.txt|.gbk|.dnaMap
pRSGEP-U6-(sg)-EF1-PuroSVCRU6EP-LHTS6C.txt|.gbk|.dnaMap
pRSGUP-U6-(sg)-UbiC-PuroSVCRU6UP-LHTS6C.txt|.gb|.dnaMap
pRSGUThy-U6-(sg)-UbiC-Thy1.1SVCRU6UThy-LHTS6C.txt|.gb|.dnaMap
Inducible sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-TagRFP-2A-PuroSVCRU6T16-LPlease Inquire.txt|.gb|.dnaMap
pRSGT17-U6Tet-(sg)-CMV-TetRep-2A-TagGFP2-2A-PuroSVCRU6T17-LPlease Inquire.txt|.gb|.dnaMap
pRSGTEP-U6Tet-(sg)-EF1-TetRep-2A-PuroSVCRU6TEP-Lno HTS.txt|.gb|.dnaMap

Dual sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRDSG16-U6M3-sg1-U6-sg2-UbiC-TagRFP-2A-PuroPlease InquirePlease Inquire.txt|.gbk|.dnaMap
Cas9-Only VectorsCatalog #Sequence**Map
pRCCH-CMV-Cas9-2A-HygroSVC9-PS.txt|.gbk|.dnaMap
pRCCB-CMV-Cas9-2A-BlastSVC9B-PS.txt|.gb|.dnaMap
pRDKCCB-CMV-dCas9-KRAB-2A-Blast (CRISPRi)SVKRABC9B-PS.txt|.gbk|.dnaMap
pRDVCCB-CMV-dCas9-VPH-2A-Blast (CRISPRa)SVVPHC9B-PS.txt|.gbk|.dnaMap

cDNA Overexpression Vectors

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cDNA Cloning VectorsCatalog #ORF PromoterMCSSequence**Map
pRCDEM-EF1-MCSPRCDEMEF1MCS.txt|.gbk|.dnaMap
pRCDEMP-EF1-MCS-2A-PuroPRCDEMPEF1TBA.txt|.gbk|.dnaMap
pRCDCMEN-CMV-MCS-EF1-NeoPRCDCMENCMVMCS.txt|.gbk|.dnaMap
pRCDCMEP-CMV-MCS-EF1-PuroPRCDCMEPCMVMCS.txt|.gbk|.dnaMap
pRCDCMER-CMV-MCS-EF1-TagRFPPRCDCMERCMVTBA.txt|.gbk|.dnaMap
pRCDCMERP-CMV-MCS-EF1-TagRFP-2A-PuroPRCDCMERPCMVTBA.txt|.gb|.dnaMap
pRCDCMEG-CMV-MCS-EF1-TagGFP2PRCDCMEGCMVTBA.txt|.gbk|.dnaMap

* Only vectors with NGS cassettes can be used for library construction.

** Sequences are available in FASTA (.txt), GenBank (.gb/.gbk), and SnapGene® (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us.

For information on the safety of our lentiviruses, please download our Lentivirus MSDS.