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Cloning and Expression Vector Information

For our cloning services, Cellecta has a wide variety of shRNA, CRISPR, CRISPRi, CRISPRa, and cDNA cloning and expression vectors to choose from. Find all vector information here, including cloning/Next-Gen Sequencing (NGS) cassette and primer design maps, sequences, GenBank/Vector NTI and SnapGene® files, and vector maps.

 

shRNA Knockdown Vectors

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Constitutive shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSI12-U6-(sh)-UbiC-TagRFP-2A-PuroDVSHC-LHTS4.txt|.gbk|.dnaMap
pRSI12N-U6-(sh)-UbiC-TagRFP-2A-NeoSVSHU612N-LHTS4.txt|.gbk|.dnaMap
pRSI16-U6-(sh)-UbiC-TagRFP-2A-PuroSVSHU616-LHTS6.txt|.gbk|.dnaMap
pRSI16cb-U6-(sh)-13kCB18-UbiC-TagRFP-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI17-U6-(sh)-UbiC-TagGFP2-2A-PuroSVSHU617-LHTS6.txt|.gbk|.dnaMap
pRSI17cb-U6-(sh)-13kCB18-UbiC-TagGFP2-2A-Puro (for clonal barcode libraries only)Not AvailableHTS6cb.txt|.gbk|.dnaMap
pRSI20-U6-(sh)-CMV-TagRFP-2A-PuroSVSHU620-LHTS6.txt|.gbk|.dnaMap
pRSI21-U6-(sh)-CMV-TagGFP2-2A-PuroSVSHU621-LHTS6.txt|.gbk|.dnaMap
pRSIUP-U6-(sh)-UbiC-PuroSVSHU6UP-LHTS6.txt|.gbk|.dnaMap
pRSI7-H1-(sh)-UbiC-TagRFP-2A-PuroSVSHH116-LPlease inquire.txt|.gbk|.dnaMap
pMSCV16URP-U6-(sh)-UbiC-TagRFP-2A-Puro (retroviral)SVSHMVU6URP-LHTS6M.txt|.gbk|.dnaMap
Inducible shRNA Knockdown VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSIT12-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T12-LHTS4.txt|.gb|.dnaMap
pRSIT16-U6Tet-(sh)-CMV-TetRep-2A-TagRFP-2A-PuroSVSHU6T16-LHTS6.txt|.gbk|.dnaMap
pRSIT17-U6Tet-(sh)-CMV-TetRep-2A-TagGFP2-2A-PuroSVSHU6T17-LHTS6.txt|.gbk|.dnaMap
pRSITEP-U6Tet-(sh)-EF1-TetRep-2A-PuroSVSHU6TEP-Lno HTS.txt|.gbk|.dnaMap
pRSITUR-U6Tet-(sh)-UbiC-TetRep-2A-TagRFPSVSHU6TUR-LHTS3.txt|.gbk|.dnaMap

 

CRISPR/Cas9 sgRNA Knockout Vectors

Single Vector sgRNA-Cas9 System

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGCCP-U6-(sg)-CMV-Cas9-2A-Puro (was pRSGC1)SVCRU6CCP-LHTS6C.txt|.gbk|.dnaMap
pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hygro (was pRSGC3)SVCRU6CCH-LHTS6C.txt|.gbk|.dnaMap
pRSGCCB-U6-(sg)-CMV-Cas9-2A-Blast (was pRSGC5)SVCRU6CCB-LHTS6C.txt|.gbk|.dnaMap
pRSGCCN-U6-(sg)-CMV-Cas9-2A-Neo (was pRSGC6)SVCRU6CCN-LHTS6C.txt|.gbk|.dnaMap
pRSGCCG-U6-(sg)-CMV-Cas9-2A-TagGFP2 (was pRSGC4)SVCRU6CCG-LHTS6C.txt|.gbk|.dnaMap
pRSGCCR-U6-(sg)-CMV-Cas9-2A-TagRFP (was pRSGC7)SVCRU6CCR-LHTS6C.txt|.gbk|.dnaMap
pRSGCUP-U6-(sg)-UbiC-Cas9-2A-Puro (was pRSGC2)SVCRU6UCP-LHTS6C.txt|.gbk|.dnaMap

Two Vector System (sgRNA and Cas9 on separate vectors)

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Constitutive sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSG16-U6-(sg)-UbiC-TagRFP-2A-PuroSVCRU616-LHTS6C.txt|.gbk|.dnaMap
pRSG17-U6-(sg)-UbiC-TagGFP2-2A-PuroSVCRU617-LHTS6C.txt|.gbk|.dnaMap
pRSG20-U6-(sg)-CMV-TagRFP-2A-PuroSVCRU620-LHTS6C.txt|.gb|.dnaMap
pRSG21-U6-(sg)-CMV-TagGFP2-2A-PuroSVCRU621-LHTS6C.txt|.gbk|.dnaMap
pRSGEP-U6-(sg)-EF1-PuroSVCRU6EP-LHTS6C.txt|.gbk|.dnaMap
Inducible sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-TagRFP-2A-PuroSVCRU6T16-LPlease Inquire.txt|.gb|.dnaMap
pRSGTEP-U6Tet-(sg)-EF1-TetRep-2A-PuroSVCRU6TEP-LPlease Inquire.txt|.gb|.dnaMap

Dual sgRNA Knockout VectorsCatalog # (Linearized)NGS Cassette*Sequence**Map
pRDSG16-U6M3-sg1-U6-sg2-TagRFP-2A-PuroPlease InquirePlease Inquire.txt|.gbk|.dnaMap
Cas9-Only VectorsCatalog #Sequence**Map
pRCCH-CMV-Cas9-2A-HygroSVC9-PS.txt|.gbk|.dnaMap
pRCCB-CMV-Cas9-2A-BlastSVC9B-PS.txt|.gb|.dnaMap
pRDKCCB-CMV-dCas9-KRAB-2A-Blast (CRISPRi)SVKRABC9B-PS.txt|.gbk|.dnaMap
pRDVCCB-CMV-dCas9-VPH-2A-Blast (CRISPRa)SVVPHC9B-PS.txt|.gbk|.dnaMap

cDNA Overexpression Vectors

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cDNA Cloning VectorsCatalog #ORF PromoterMCSSequence**Map
pRCDEM-EF1-MCSPRCDEMEF1MCS.txt|.gbk|.dnaMap
pRCDEMP-EF1-MCS-2A-PuroPRCDEMPEF1TBA.txt|.gbk|.dnaMap
pRCDCMEN-CMV-MCS-EF1-NeoPRCDCMENCMVMCS.txt|.gbk|.dnaMap
pRCDCMEP-CMV-MCS-EF1-PuroPRCDCMEPCMVMCS.txt|.gbk|.dnaMap
pRCDCMER-CMV-MCS-EF1-TagRFPPRCDCMERCMVTBA.txt|.gbk|.dnaMap
pRCDCMERP-CMV-MCS-EF1-TagRFP-2A-PuroPRCDCMERPCMVTBA.txt|.gb|.dnaMap
pRCDCMEG-CMV-MCS-EF1-TagGFP2PRCDCMEGCMVTBA.txt|.gbk|.dnaMap

* Only vectors with NGS cassettes can be used for library construction.

** Sequences are available in FASTA (.txt), GenBank (.gbk), and SnapGene® (.dna) formats. You may use the free SnapGene Viewer to open .dna files. Please contact us if you’d like us to provide another format.

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