DriverMap™ Targeted Expression Profiling Service
Cellecta’s DriverMap™ service is a novel, comprehensive end-to-end, whole-genome targeted expression profiling solution intended for research applications. This quantitative, multiplex approach leverages the power of Next-Generation Sequencing (NGS) and PCR technologies to allow the researcher to obtain ultra-sensitive digital expression profiling data for all protein-coding genes in the human genome.
For more information on the DriverMap RNA Profiling Technology, please see our DriverMap RNA Profiling Technology page. We also offer a DriverMap assay kit for simple expression profiling of 24 or 96 samples in your lab.
Cellecta’s DriverMap RNA Expression Profiling service includes:
- RNA isolation
- Sample QC
- RT-PCR of samples using the DriverMap primers
- Next-generation sequencing (NGS) run
- Analysis to yield gene expression levels
The analysis includes deconvolution and normalization of the expression levels for each target gene. The genes are normalized against a set of some 70 housekeeping genes across all samples.
Since the DriverMap assay is a targeted RT-PCR approach that generates a single amplicon of approximately 200 bases for each target gene, the library only has about 19,000 sequences. This is very different from RNA-Seq, where you essentially sequence the entire transcriptome. As a result, with the DriverMap assay, you can achieve a very good depth of coverage and more sensitivity than RNA-Seq, with 5-10 fold fewer reads. We typically obtain about 5 million reads per sample.
If you are interested in sending samples for Driver Map expression profiling, please download and complete the order form and we will provide you a quote. We accept isolated RNA samples, as well as cells or tissue samples.