DriverMap™ Targeted Expression Profiling Service
Combining the sensitivity and dynamic range of qPCR with the throughput and scale of NGS.
Cellecta’s DriverMap™ service is a novel, comprehensive end-to-end, whole-genome targeted expression profiling solution intended for research applications. This quantitative, multiplex approach leverages the power of Next-Generation Sequencing (NGS) and PCR technologies to allow the researcher to obtain ultra-sensitive digital expression profiling data for all protein-coding genes in the human genome.
For more information on the DriverMap RNA Profiling Technology, please see our DriverMap RNA Profiling Technology page. We also offer a DriverMap assay kit for simple expression profiling of 24 or 96 samples in your lab.
Cellecta’s DriverMap RNA Expression Profiling service includes:
- RNA isolation
- Sample QC
- RT-PCR of samples using the DriverMap primers
- Next-generation sequencing (NGS) run
- Analysis to yield gene expression levels
The analysis includes deconvolution and normalization of the expression levels for each target gene. The genes are normalized against a set of some 70 housekeeping genes across all samples.
Since the DriverMap assay is a targeted RT-PCR approach that generates a single amplicon of approximately 200 bases for each target gene, the library only has about 19,000 sequences. This is very different from RNA-Seq, where you essentially sequence the entire transcriptome. As a result, with the DriverMap assay, you can achieve a very good depth of coverage and more sensitivity than RNA-Seq, with 5-10 fold fewer reads. We typically obtain about 5 million reads per sample.
For more details of what you receive, a full sample results report is available upon request.
If you are interested in sending samples for Driver Map expression profiling, please download and complete the order form and we will provide you with a quote. We accept isolated RNA samples, as well as cells or tissue samples.