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Lentiviral Packaging Services

Cellecta can provide any of its lentiviral constructs or libraries as ready-to-transduce, pre-packaged as VSV-g pseudotyped lentiviral particles. These lentiviral preparations can be directly added to cells in culture. The lentiviral particles fuse with the cell membranes and the lentiviral RNA enters the cell, is reverse-transcribed, and integrated into the cell’s DNA.

Lentiviral expression constructs packaged into pseudoviral particles can be transduced into cells with very high efficiency, approaching 100% in some cell types. Packaged lentiviral constructs can be transduced into even the most difficult to transfect cells, such as primary, stem, and differentiated cells with high efficiency.

We can provide various amounts of virus depending on your needs.

Pricing  

Please note that pricing for our packaging service depends on the total number of viral particles you want regardless of the volume. For example, we can provide 100 million (10e8) transduction units (TU) in 10 ml of 10e7 each (i.e., 10^7/ml), or in a single ml of 10e8 (i.e., 10^8/ml). With both variations, we provide the same amount of total virus so the price does not change.  Typically, we provide ca. 2x10^7 TU to 5x10^7 TU/ml. However, if you would prefer higher or lower concentrations, we will do our best to accommodate you.

Standard Preparative Scales

The standard preparative scales are indicated below and are based on the total amount of lentiviral particles we provide. The volumes will vary slightly with the preparation. Again, depending on your needs, we can provide the viral preparation more concentrated or diluted if requested.

 

Individual and Library Lentiviral Packaging Service Quantity provided Lead time

Lentiviral Library Packaging of Custom shRNA/sgRNA Library (Small Scale)

> 5 x 10^7 TU

quote

Lentiviral Library Packaging of Custom shRNA/sgRNA Library

> 1 x 10^8 TU

3 weeks

Lentiviral Library Packaging of Custom shRNA/sgRNA Library

> 2 x 10^8 TU

3 weeks

Lentiviral Library Packaging of Custom shRNA/sgRNA Library

> 5 x 10^8 TU

3 weeks

Lentiviral Library Packaging of Custom shRNA/sgRNA Library (Large Scale)

> 1 x 10^9 TU

3 weeks

 

 

Lentiviral Library Packaging of Custom Single-Vector sgRNA-Cas9 Library

> 1 x 10^7 TU

4 weeks

Lentiviral Library Packaging of Custom Single-Vector sgRNA-Cas9 Library

> 2 x 10^7 TU

4 weeks

Lentiviral Library Packaging of Custom Single-Vector sgRNA-Cas9 Library

> 5 x 10^7 TU

4 weeks

Lentiviral Library Packaging of Custom Single-Vector sgRNA-Cas9 Library (Large Scale)

> 1 x 10^8 TU

4 weeks

 

Viral titers are based on actual transduction of viral preparation into 293K cells. We do not use estimates based on PCR amplification of viral DNA or p21 detection of viral proteins, except with custom constructs that do not contain a fluorescent marker or antibiotic selection.

 

Our service is set up for packaging of our extensive line of lentiviral vectors. However, we can also package third-party vectors provided they are compatible with our packaging system

 

If you need a construct or library packaged, order now, or contact us for a quote.

We also offer ancillary lentiviral packaging products