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Human Genome-Wide shRNA Library

Cellecta offers a Pooled Lentiviral Human Genome-Wide shRNA library targeting nearly all protein-encoding genes. This shRNA library is the most current pooled lentiviral shRNA library available on the market. It covers all genes in the human genome, and each gene is targeted by 8 hairpins. We have incorporated clonal barcodes to enable you to track growth, differentiation, or migration of specific cells containing a specific shRNA throughout your experiment.

For Genome-Wide Coverage in Human

  • 3 Modules, each covering approximately 6,500 genes
  • Each gene is targeted by 8 hairpins for a total of approximately 55,000 hairpins per module
  • Modules are made with non-overlapping barcodes so that they can be combined to form a complete genome-wide shRNA library
  • Targets all (19,276) protein encoding genes

Intelligent Barcode Design, Quality Data

  • 22-nt barcodes facilitate Next-Gen Sequencing data analysis and identification of functional shRNAs
  • Compatible with Illumina HiSeq, GAIIx, and NextSeq NGS systems
  • Barcodes are identified and converted to lists of genes/shRNA with enumerated barcode data
  • Amplification of shRNA hairpins is not required

Library Modules for Intelligent Loss-of-Function Screening

  • hGW Module 1 targets signal transduction genes
    • Members of major signal transduction pathways
    • Top-ranking genes from the Cancer Genome Atlas
    • Approved drug targets
    • Many disease-associated genes
  • hGW Module 2 targets disease-associated genes and known drug targets
  • hGW Module 3 targets cell surface markers, extracellular matrix genes, and DNA binding genes

The target gene lists for the Cellecta hGW shRNA Library Modules can be downloaded here:

 

shRNA Library VectorNGS CassetteSequence (.txt | .gbk | .dna*)Map
pRSI16cb-U6-sh-13kCB18-HTS6cb-UbiC-TagRFP-2A-PuroHTS6cbFASTA | GenBank | SnapGeneMap

* Download the SnapGene® Viewer to open .dna format files.

User Manuals

  • Human Genome-Wide Pooled Lentiviral shRNA Libraries, v4b (PDF)
  • Pooled Lentiviral shRNA Library Screening Reference Manual, v2a (PDF)

Product Analysis Certificates (PACs) are provided as PDF files and are available for download on the Product Manuals and Certificates page under the Resources section.

Next-Gen Sequencing and Analysis

Cellecta libraries are provided with a complete protocol and all sequencing information to enable researchers to perform high throughput genetics screens and analysis. However, we also do provide Next-Gen Sequencing and analysis services for researchers running their own screens with our libraries. Just provide harvested cells for each time point or treatment condition (one sample), and we extract DNA, amplify, sequence, and assemble the data with some basic analysis.

You provide Cellecta with frozen cell pellets or tissue after screening…

…and Cellecta does the rest:

  • Extracts genomic DNA
  • Amplifies barcodes
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerate shRNA counts from raw sequencing data
  • Additional bioinformatics and pathway analysis available

Please see the Next-Gen Sequencing and Analysis web page for additional information and how to order.

Available Formats

 

Plasmid Library

Catalog NumberItem DescriptionAmount Provided
HGW-M1-P2Human Genome-Wide shRNA Library, Module 1 (plasmid)200 μg
HGW-M2-P2Human Genome-Wide shRNA Library, Module 2 (plasmid)200 μg
HGW-M3-P2Human Genome-Wide shRNA Library, Module 3 (plasmid)200 μg

 

Packaged Viral Library

Catalog NumberItem DescriptionAmount Provided
HGW-M1-V8Human Genome-Wide shRNA Library, Module 12 x 108 TU
HGW-M2-V8Human Genome-Wide shRNA Library, Module 22 x 108 TU
HGW-M3-V8Human Genome-Wide shRNA Library, Module 32 x 108 TU
HGW-M1-V9Human Genome-Wide shRNA Library, Module 11 x 109 TU
HGW-M2-V9Human Genome-Wide shRNA Library, Module 21 x 109 TU
HGW-M3-V9Human Genome-Wide shRNA Library, Module 31 x 109 TU

What you will receive in addition to your order:

  • Plasmid orders include 10 μg empty library vector, for cloning individual constructs used to validate hits from your screen.
  • Virus orders include an additional small aliquot of virus to be used for titering in your target cells

 

Related Products:

Catalog NumberItem DescriptionPrice
CPCP-K2AReady-to-Use Lentiviral Packaging Plasmid Mix, 250 μg (for 25 10-cm plates)$325
LFVC1LentiFuge™ Viral Concentration Reagent, 1 ml (for 1 liter supernatant)$325

Please email sales@cellecta.com for questions or to obtain a quote.